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Relationship of Bovine Lymphocyte Antigen Genes with Clinical Mastitis Disease Using SSCP Technique

  • ANIMAL GENETICS
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Abstract

BoLA-A and BoLA-DRB3 are in class I and II genes of bovine lymphocyte antigen of immune system respectively which cattle express per haplotype. The aim of this study was to evaluate relationship of BoLA-A and BoLA-DRB3 polymorphisms with clinical mastitis disease using SSCP technique. In the current study, blood sampling was carried out from Holstein cattle (n = 50 susceptible and n = 50 resistant to clinical mastitis) and a random sampling was conducted from Iranian native cattle (Sarabi; n = 50). Amplification of exon 2 of BoLA-DRB3 and BoLA-A genes by specific primers was used for SSCP technique. SSCP technique successfully able to detect some pattern is related to clinical mastitis disease in BoLA-DRB3.2 region as well as genotype pattern of C was observed significantly (P < 0.05) in susceptible Holstein cattle in compared to others pattern. Also, B and Q genotypes patterns of SSCP were detected only in susceptible cattle. Also, SSCP results were showed 17 genotypes pattern in Holstein and 17 different genotypes pattern in Sarabi cattle in BoLA-DRB3.2 gene. Thus, our finding indicated that Sarabi breed comprises completely different allelic in the BoLA-DRB3.2 region compared to Holstein breed. Moreover, results of this study revealed that exon 2 of BoLA-A showed lack of polymorphism in both studied breed. This is the first study on characterization of exon 2 of BoLA-A‎gene using SSCP technique in bovine.‎

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ACKNOWLEDGMENTS

This work was funded by University of Tabriz, Iran.

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Authors and Affiliations

  1. Department of Pathobiology, Faculty of Veterinary Medicine, University of Tabriz, 5166616471, Tabriz, Iran

    M. Firouzamandi

  2. Veterinary Reproductive and Obstetrics, University of Shiraz, Shiraz, Iran

    D. Eshghi

  3. Department of Clinical Science, University of Tabriz, 5166616471, Tabriz, Iran

    M. Toloui & R. Asadpour

Authors
  1. M. Firouzamandi
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  2. D. Eshghi
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  3. M. Toloui
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  4. R. Asadpour
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Correspondence to M. Firouzamandi.

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Firouzamandi, M., Eshghi, D., Toloui, M. et al. Relationship of Bovine Lymphocyte Antigen Genes with Clinical Mastitis Disease Using SSCP Technique. Russ J Genet 55, 473–479 (2019). https://doi.org/10.1134/S1022795419040033

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