Skip to main content
SpringerLink
Log in

Molecular genetic analysis of five extant reserves of black honeybee Apis melifera melifera in the Urals and the Volga region

  • Animal Genetics
  • Published:
Russian Journal of Genetics Aims and scope Submit manuscript

Abstract

Local populations of the black honeybee Apis mellifera mellifera from the Urals and the Volga region were examined in comparison with local populations of southern honeybee subspecies A. m. caucasica and A. m. carpatica from the Caucasus and the Carpathians. Genetic analysis was performed on the basis of the polymorphism of nine microsatellite loci of nuclear DNA and the mtDNA COI–COII locus. On the territory of the Urals and the Volga region, five extant populations (reserves) of the black honeybee A. m. mellifera were identified, including the Burzyanskaya, Tatyshlinskaya, Yuzhno-Prikamskaya, Visherskaya, and Kambarskaya populations. These five populations are the basis of the modern gene pool of the black honeybee A. m. mellifera from the Urals and the Volga region. The greatest proportion of the remaining indigenous gene pool of A. m. mellifera (the core of the gene pool of the population of A. m. mellifera) is distributed over the entire territory of Perm krai and the north of the Republic of Bashkortostan. For the population of A. m. mellifera from the Urals and the Volga region, the genetic standards were calculated, which will be useful for future population studies of honeybees.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Sheppard, W.S. and Meixner, M.D., Apis mellifera pomonella, a new honey bee subspecies from Central Asia, Apidologie, 2003, vol. 34, pp. 367–375.

    Article  Google Scholar 

  2. Meixner, M.D., Leta, M.A., Koeniger, N., and Fuchs, S., The honey bees of Ethiopia represent a new subspecies of Apis mellifera—Apis mellifera simensis n. ssp., Apidologie, 2011, vol. 42, pp. 425–437.

    Article  Google Scholar 

  3. Nikolenko, A.G. and Poskryakov, A.V., Polymorphism of locus COI–COII of mitochondrial DNA in the honeybee Apis mellifera L. from the Southern Ural region, Russ. J. Genet., 2002, vol. 38, no. 4, pp. 364–368.

    Article  CAS  Google Scholar 

  4. Büchler, R., Costa, C. Hatjina, F., et al., The influence of genetic origin and its interaction with environmental effects on the survival of Apis mellifera L. colonies in Europe, J. Apic. Res., 2014, vol. 53, pp. 205–214.

    Article  Google Scholar 

  5. Jensen, A.B., Palmer, K.A., Boomsma, J.J., and Pedersen, B.V., Varying degrees of Apis mellifera ligustica introgression in protected populations of the black honeybee, Apis mellifera mellifera, in northwest Europe, Mol. Ecol., 2005, vol. 14, pp. 93–106.

    Article  PubMed  Google Scholar 

  6. Soland-Reckeweg, G., Heckel, G., Neumann, P., et al., Gene flow in admixed populations and implications for the conservation of the western honeybee, Apis mellifera, J. Insect Conserv., 2009, vol. 13, pp. 317–328.

    Article  Google Scholar 

  7. Muñoz, I., Dall’Olio, R., Lodesani, M., and De La Rúa, P., Population genetic structure of coastal Croatian honeybees (Apis mellifera carnica), Apidologie, 2009, vol. 40, pp. 617–626.

    Article  Google Scholar 

  8. Nedic, N., Francis, R.M., Stanisavljevic, L., et al., Detecting population admixture in the honey bees of Serbia, J. Apic. Res., 2014, vol. 53, pp. 303–313.

    Article  Google Scholar 

  9. Uzunov, A., Meixner, M.D., Kiprijanovska, H., et al., Genetic structure of Apis mellifera macedonica population based on microsatellite DNA polymorphism, J. Apic. Res., 2014, vol. 53, no. 2, pp. 288–295.

    Article  CAS  Google Scholar 

  10. Jensen, A.B. and Pedersen, B.V., Honeybee conservation: a case story from Læsø island, Denmark, Beekeeping and Conserving Biodiversity of Honeybee: Sustainable Bee Breeding, Lodesani, M. and Costa, C., Hebden Bridge: Northern Bee Books, 2005, pp. 142–164.

  11. Maul, V. and Hähnle, A., Morphometric studies with pure bred stock of Apis mellifera carnica Pollmann from Hessen, Apidologie, 1994, vol. 25, pp. 119–132.

    Article  Google Scholar 

  12. Il’yasov, R.A., Petukhov, A.V., Poskryakov, A.V., and Nikolenko, A.G., Local honeybee (Apis mellifera mellifera L.) populations in the Urals, Russ. J. Genet., 2007, vol. 43, no. 6, pp. 709–711.

    Article  Google Scholar 

  13. Il’yasov, R.A., Kosarev, M.N., and Yumaguzhin, F.G., Burzyan wild-hive honeybee and the wild-hive apiculture in the South Urals, Pchelovodstvo, 2015, no. 7, pp. 12–15.

    Google Scholar 

  14. Petukhov, A.V., Shurakov, A.I., Es’kov, E.K., et al., Morphological characteristics of the Central Russian bees from Upper Kama population, Pchelovodstvo, 1996, no. 5, pp. 8–10.

    Google Scholar 

  15. Nikonorov, Yu.M., Ben’kovskaya, G.V., Poskryakov, A.V., et al., The use of the PCR technique for control of pure-breeding of honeybee (Apis mellifera mellifera L.) colonies from the Southern Urals, Russ. J. Genet., 1998, vol. 34, no. 11, pp. 1344–1348.

    CAS  Google Scholar 

  16. Il’yasov, R.A. and Poskryakov, A.V., Phylogenetics of Apis mellifera subspecies, Pchelovodstvo, 2006, no. 7, pp. 18–19.

    Google Scholar 

  17. Il’yasov, R.A., Poskryakov, A.V., Kolbina, L.M., and Nikolenko, A.G., Conservation of Apis mellifera mellifera L. in the Udmurt Republic, Pchelovodstvo, 2007, no. 6, pp. 13–14.

    Google Scholar 

  18. Kolbina, L.M., Nepeivoda, S.N., Vorob’eva, S.L., et al., Genetic differentiation of Apis mellifera L. populations in Udmurt Republic, Agrar. Nauka Evro- Severo-Vostoka, 2011, vol. 6, no. 25, pp. 46–50.

    Google Scholar 

  19. Brandorf, A.Z., Ivoilova, M.M., Il’yasov, R.A., et al., Population genetic differentiation of honey bees in Kirov oblast, Pchelovodstvo, 2012, no. 7, pp. 14–16.

    Google Scholar 

  20. Il’yasov, R.A., Poskryakov, A.V., Petukhov, A.V., and Nikolenko, A.G., Genetic differentiation of local populations of the dark European bee Apis mellifera mellifera L. in the Urals, Russ. J. Genet., 2015, vol. 51, no. 7, pp. 677–682.

    Article  Google Scholar 

  21. Il’yasov, R.A., Poskryakov, A.V., and Nikolenko, A.G., New SNP markers of the honeybee vitellogenin gene (Vg) used for identification of subspecies Apis mellifera mellifera L., Russ. J. Genet., 2015, vol. 51, no. 2, pp. 163–168.

    Article  Google Scholar 

  22. Estoup, A., Garnery, L., Solignac, M., and Cornuet, J.-M., Microsatellite variation in honey bee (Apis mellifera L.) populations: hierarchical genetic structure and test of the infinite allele and stepwise mutation models, Genetics, 1995, vol. 140, pp. 679–695.

    CAS  PubMed  PubMed Central  Google Scholar 

  23. Haberl, M. and Tautz, D., Tri- and tetranucleotide microsatellite loci in honey bees (Apis mellifera)—a step towards quantitative genotyping, Mol. Ecol., 1999, vol. 8, pp. 1351–1362.

    Article  Google Scholar 

  24. Solignac, M., Vautrin, D., Loiseau, A., et al., Five hundred and fifty microsatellite markers for the study of the honeybee (Apis mellifera L.) genome, Mol. Ecol. Notes, 2003, vol. 3, pp. 307–311.

    Article  CAS  Google Scholar 

  25. Garnery, L., Solignac, M., Celebrano, G., and Cornuet, J.-M., A simple test using restricted PCR-amplified mitochondrial DNA to study the genetic structure of Apis mellifera L., Experientia, 1993, vol. 49, pp. 1016–1021.

    Article  CAS  Google Scholar 

  26. Pritchard, J.K., Stephens, M., and Donnelly, P., Inference of population structure using multilocus genotype data, Genetics, 2000, vol. 155, pp. 945–959.

    CAS  PubMed  PubMed Central  Google Scholar 

  27. Franck, P., Garnery, L., Solignac, M., and Cornuet, J.M., The origin of West European subspecies of honeybees (Apis mellifera): new insights from mitochondrial DNA and microsatellite data, Evolution, 1998, vol. 52, no. 4, pp. 1119–1134.

    Article  CAS  Google Scholar 

  28. Garnery, L., Franck, P., Baudry, E., et al., Genetic biodiversity of the West European honeybee (Apis mellifera mellifera and A. m. iberica): 1. Mitochondrial DNA, Genet. Sel. Evol., 1998, vol. 30, pp. 31–47.

    Article  Google Scholar 

  29. Franck, P., Garnery, L., Loiseau, A., et al., Genetic diversity of the honeybee in Africa: microsatellite and mitochondrial data, Heredity, 2001, vol. 86, pp. 420–430.

    Article  CAS  PubMed  Google Scholar 

  30. Franck, P., Garnery, L., Celebrano, G., et al., Hybrid origins of the Italian honeybees, Apis mellifera ligustica and A. m. sicula, Mol. Ecol., 2000, vol. 9, pp. 907–923.

    CAS  Google Scholar 

  31. Pihler, I., Kiprijanovska, H., Plavsa, N., et al., Population- genetical characteristics of the bee population of Vojvodina, Genetika, 2014, vol. 64, no. 1, pp. 219–226.

    Article  Google Scholar 

  32. Yin, L., Ji, T., Chen, G., and Peng, W., Genetic characterization of three breeds of high royal jelly producing honeybee (Apis mellifera ligustica) in China, Afr. J. Agric. Res., 2011, vol. 6, no. 2, pp. 331–337.

    Google Scholar 

  33. Bodur, Ç., Genetic Structure Analysis of Honeybee Populations Based on Microsatellites: A Thesis for the Degree of Doctor of Philosophy in Biology, The Graduate School of Natural and Applied Sciences of Middle East Technical University, 2005.

    Google Scholar 

  34. Galindo-Cardona, A., Acevedo-Gonzales, J.P., Rivera-Marchand, B., and Giray, T., Genetic structure of the gentle africanized honey bee population (gAHB) in Puerto Ricco, BMC Genet., 2013, vol. 14, pp. 1–12.

    Article  Google Scholar 

  35. Estoup, A., Solignac, M., and Cornuet, J.-M., Precise assessment of the number of patrilines and of genetic relatedness in honeybee colonies, Proc. R. Soc. London, Ser. B, 1994, vol. 258, pp 1–7.

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Institute of Biochemistry and Genetics, Ufa Scientific Center, Russian Academy of Sciences, Ufa, Republic of Bashkortostan, 450054, Russia

    R. A. Ilyasov, A. V. Poskryakov & A. G. Nikolenko

  2. Department of Zoology, Perm State Humanitarian Pedagogical University, Perm, 614990, Russia

    A. V. Petukhov

Authors
  1. R. A. Ilyasov
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. A. V. Poskryakov
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. A. V. Petukhov
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. A. G. Nikolenko
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to R. A. Ilyasov.

Additional information

Original Russian Text © R.A. Ilyasov, A.V. Poskryakov, A.V. Petukhov, A.G. Nikolenko, 2016, published in Genetika, 2016, Vol. 52, No. 8, pp. 931–942.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Ilyasov, R.A., Poskryakov, A.V., Petukhov, A.V. et al. Molecular genetic analysis of five extant reserves of black honeybee Apis melifera melifera in the Urals and the Volga region. Russ J Genet 52, 828–839 (2016). https://doi.org/10.1134/S1022795416060053

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1134/S1022795416060053

Keywords

Search

Navigation