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Comparative analysis of DNA homology in pericentric regions of chromosomes of wood mice from genera Apodemus and Sylvaemus

  • Animal Genetics
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Abstract

In the present study, an analysis of the DNA homology of the pericentric chromosomal regions and pericentric heterochromatin in distantly related species of wood mice (species from the Apodemus genus, as well as from the Apodemus and Sylvaemus genera) was conducted by fluorescent in situ hybridization (FISH) of microdissected DNA probes obtained from the corresponding chromosomal regions of these species. Cross-hybridization of microdissected DNA probes obtained from pericentric C-positive blocks of chromosomes of Sylvaemus species with chromosomes of Apodemus species, as well as DNA probes from pericentric C-positive blocks of chromosomes of Apodemus species with chromosomes of Apodemus and Sylvaemus species, showed that DNA repeats homologous to the pericentric regions in other species represented dispersed repeats in C-negative chromosomal regions, as well as in several regions bordering pericentric Cpositive and C-negative regions in sex chromosomes and autosomes and in distal regions of the long arms of several autosomes. The results indicate that the level of DNA homology in pericentric chromosomal regions decreases with an increase in the differentiation level and a decrease in the relationship between the compared forms and species of wood mice. Most likely, degeneration of the DNA repeats is accompanied by a gradual destruction of repeat clusters and their replacement by new, nonhomologous repeats in almost all pericentric regions (some old repetitive sequences might be “extruded” into interstitial or telomeric regions of chromosomes). These processes, which are observed in some species from Sylvaemus genus, in distantly related species of Sylvaemus and Apodemus genera have almost achieved the final stages.

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Authors and Affiliations

  1. Institute of Cytology and Genetics, Siberian Branch, Russian Academy of Sciences, Novosibirsk, 630090, Russia

    N. B. Rubtsov, T. V. Karamysheva & M. N. Bochkarev

  2. Department of Cytology and Genetics, Novosibirsk State University, Novosibirsk, 630090, Russia

    N. B. Rubtsov

  3. Koltzov Institute of Developmental Biology, Russian Academy of Sciences, Moscow, 119334, Russia

    A. S. Bogdanov

  4. Institute of Biology and Soil Science, Far Eastern Branch, Russian Academy of Sciences, Vladivostok, 690022, Russia

    I. V. Kartavtseva

  5. Nihon University, Kameino 1866, Fujisawa, Kanagawa, 252-8510, Japan

    M. A. Iwasa

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  1. N. B. Rubtsov
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Original Russian Text © N.B. Rubtsov, T.V. Karamysheva, A.S. Bogdanov, I.V. Kartavtseva, M.N. Bochkarev, M.A. Iwasa, 2015, published in Genetika, 2015, Vol. 51, No. 12, pp. 1423–1432.

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Rubtsov, N.B., Karamysheva, T.V., Bogdanov, A.S. et al. Comparative analysis of DNA homology in pericentric regions of chromosomes of wood mice from genera Apodemus and Sylvaemus . Russ J Genet 51, 1233–1242 (2015). https://doi.org/10.1134/S1022795415120091

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  • DOI: https://doi.org/10.1134/S1022795415120091

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