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Gene Expression of Metal Transporting P-Type ATPases HMA2 and HMA4 in Brassica juncea L. Grown at Different Zn Levels

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Abstract

The Brassicaceae plant family includes some of the most economically important crops with wide-ranging adaptability for cultivation under various agro-climatic conditions. Among 25% of discovered heavy metal hyperaccumulators belong to this family. Brassica juncea (L.) Czern. hyperaccumulate metals in leaves and thus has a great potential for phytoremediation of metals from contaminated soils. In this study, B. juncea plants were grown in the soil at different zinc concentrations (0–500 µM). Plants showed no toxicity symptoms and accumulated a significant amount of Zn in the leaves. Gene expression analysis showed that increased Zn levels in the leaves trigger the expression of the HMA2 and HMA4 genes, suggesting that they play an essential role in Zn detoxification and sequestration at the subcellular level. Furthermore, the bioinformatics analysis of these transporters in B. juncea showed a significant similarity at gene and protein levels to that of HMA2 and HMA4 transporters in other members of the Brassicaceae family. The data indicates the importance of these transporters in Zn sequestration and detoxification in accumulator plants in Brassicaceae.

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ACKNOWLEDGMENTS

We appreciate the help given by Amar Cemanovic, Istanbul Technical University, Istanbul in some experimental procedures.

Funding

This work was supported by the Usak University Scientific Research Project (BAP) grant given to Prof. Dr. Abdulrezzak Memon (project no. BAP-2017/TP042).

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Authors and Affiliations

  1. Department of Molecular Biology and Genetics, Faculty of Arts and Science, Usak University, Usak, Turkey

    N. Meraklı & A. Memon

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  1. N. Meraklı
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Contributions

Concept: ARM, Desing: ARM, NM, Execution: ARM, NM, Material supplying: ARM, Data acquisition: ARM, NM, Writing: ARM, NM, Critical review: ARM.

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Correspondence to A. Memon.

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This article does not contain any studies involving animals or human participants as objects of research.

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The authors declare that they have no competing interests.

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Meraklı, N., Memon, A. Gene Expression of Metal Transporting P-Type ATPases HMA2 and HMA4 in Brassica juncea L. Grown at Different Zn Levels. Russ J Plant Physiol 70, 34 (2023). https://doi.org/10.1134/S102144372260283X

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