Skip to main content
SpringerLink
Log in

Physiological (Integral) Approach to the Use of Chlorophyll Fluorescence Parameters of Plant Leaves

  • RESEARCH PAPERS
  • Published:
Russian Journal of Plant Physiology Aims and scope Submit manuscript

Abstract

Possible use of integral characteristics of slow stage of chlorophyll fluorescence induction (CFI) for monitoring of physiological state of plants in phytocenoses was studied in the leaves of wheat Triticum aestivum L. and radish Raphanus sativus var. radicula Pers., cv. Virovskii belyi, grown in artificial-light culture. Investigation concerned the behavior of Т0.5 (half-time of fluorescence intensity decrease from its peak to a steady-state level), Rfd (fluorescence decrease ratio also known as vitality index), parameter \({{{{\overline V }}_{{{\text{max}}}}}}\) (maximum possible average rate of induction transient), and \(\bar {\alpha }\) (average efficiency of PSA in the induction period) during leaf ontogenesis. Plant material was the discs cut from uniformly illuminated wheat leaves of different age from the top (sixth) storey of plants and the disks from the second (in the order of emergence) true leaves of radish at the age from 3 to 24 days. The plants were grown in controlled-climate chambers in hydroponic culture on claydite at irradiance of 100 W/m2 of photosynthetically active radiation (PAR). CFI curves were recorded using a single-beam unit. Values of parameter Т0.5 slightly changed during leaves’ ontogenesis up to the time of senescence (the age of 26 days). Relative changes in the values of CFI parameters (Δ rel., %) at the age from 2 to 24 days were 28% for \(\bar {\alpha }\) and 30% for Т0.5. For \({{{{\overline V }}_{{{\text{max}}}}}}\) and Rfd, the values were 67 and 70%, respectively. Upon more advanced ageing and comparison of parameters for 24- and 26-day-old leaves, more pronounced changes were observed for Т0.5 (by 37.6%) and \({{{{\overline V }}_{{{\text{max}}}}}}\) (by 36.0%) and less pronounced for \(\bar {\alpha }\) (by 23.0%) and Rfd (by 13.0%). Thus, the pattern of parameter Т0.5 behavior observed in the course of wheat leaves’ ontogenesis distinguished it from other fluorescence parameters of CFI slow stage (Rfd, \(\bar {\alpha }\), and \({{{{\overline V }}_{{{\text{max}}}}}}\)); this gives grounds for a potential use of Т0.5 as one of the simplest integral indicators of physiological state and pathophysiological (irreversible) changes occurring in plant leaves within a wide age interval and upon senescence. Unambiguous interpretation of the data obtained by means of parameter Т0.5 under stress conditions for estimation of irreversibility of the observed changes in PSA requires more detailed investigations.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1.

Similar content being viewed by others

REFERENCES

  1. Goltsev, V.N., Kalaji, H.M., Paunov, M., Bąba, W., Horaczek, T., Mojski, J., Kociel, H., and Allakhverdiev, S.I., Variable chlorophyll fluorescence and its use for assessing physiological condition of plant photosynthetic apparatus, Russ. J. Plant Physiol., 2016, vol. 63, p. 869. https://doi.org/10.1134/S1021443716050058

    Article  CAS  Google Scholar 

  2. Roháček, K., Chlorophyll fluorescence parameters: the definitions, photosynthetic meaning, and mutual relationships, Photosynthetica, 2002, vol. 40, p. 13. https://doi.org/10.1023/A:1020125719386

    Article  Google Scholar 

  3. Ptushenko, V.V., Karavaev, V.A., Solntsev, M.K., and Tikhonov, A.N., Biophysical methods of ecological monitoring. Photosynthetic characteristics of tree plants growing in Moscow city, Biophysics (Moscow), 2013, vol. 58, p. 228.

    Article  CAS  Google Scholar 

  4. Nesterenko, T.V., Tikhomirov, A.A., and Shikhov, V.N., Induction of chlorophyll fluorescence and evaluation of plant resistance to stress factors, Zh. Obshch. Biol., 2007, vol. 68, p. 455.

    Google Scholar 

  5. Saakov, V.S., Energetics of green cell stress resistance: a concept, Dokl. Biol. Sci., 2000, vol. 375, p. 613.

    Article  CAS  Google Scholar 

  6. Semikhatova, O.A., Energy aspects of the integration of physiological processes in a plant, Fiziol. Rast., 1980, vol. 27, p. 1105.

    Google Scholar 

  7. Mokronosov, A.T., Ontogeneticheskii aspekt fotosinteza (Ontogenetic Aspect of Photosynthesis), Moscow: Nauka, 1981.

  8. Wu, A., Hammer, G.L., Doherty, A., von Cammerer, S., and Farquhar, G.D., Quantifying impacts of enhancing photosynthesis on crop yield, Nat. Plants, 2019, vol. 5, p. 380. https://doi.org/10.1038/s41477-019-0398-8

    Article  PubMed  Google Scholar 

  9. Ivanchenko, V.M., Marshakova, M.I., Urbanovich, T.A., Mikul’skaya, S.A., and Obukhovskaya, L.V., Reciprocal influence of chloroplasts and mitochondria on energy-transforming functions, Dokl. Akad. Nauk SSSR, 1986, vol. 291, p. 505.

    CAS  Google Scholar 

  10. Rakhmankulova, Z.F., The relationship between photosynthesis and respiration of the whole plant in normal conditions and under environmental stress, Zh. Obshch. Biol., 2002, vol. 63, p. 44.

    Google Scholar 

  11. Rakhmankulova, Z.F., Physiological aspects of photosynthesis–respiration interrelations, Russ. J. Plant Physiol., 2019, vol. 66, p. 365. https://doi.org/10.1134/S1021443719030117

    Article  CAS  Google Scholar 

  12. Bulgakov, N.G., Indication of the state of natural ecosystems and regulation of environmental factors: a review of existing approaches, Usp. Sovrem. Biol., 2002, vol. 122, p. 115.

    CAS  Google Scholar 

  13. Nesterenko, T.V., Shikhov, V.N., and Tikhomirov, A.A., Estimation of changes in the activity of photosynthetic apparatus of plant leaves based on half-time of fluorescence intensity decrease, Photosynthetica, 2019, vol. 57, p. 132. https://doi.org/10.32615/ps.2019.005

    Article  CAS  Google Scholar 

  14. Nesterenko, T.V., Shikhov, V.N., and Tikhomirov, A.A., The fluorescence method for determining of photosynthetic apparatus reactivity in plant leaves, Zh. Obshch. Biol., 2019, vol. 80, p. 187. https://doi.org/10.1134/S0044459619030060

    Article  Google Scholar 

  15. Thomas, H. and Stoddart, J.L., Leaf senescence, Annu. Rev. Plant Physiol., 1980, vol. 31, p. 83.

    Article  CAS  Google Scholar 

  16. Romanova, A.K., Semenova, G.A., Novichkova, N.S., Ignat’ev, A.R., Mudrik, V.A., and Ivanov, B.N., Physiological, biochemical, and fluorescence parameters of senescing sugar beet leaves in the vegetative phase of growth, Russ. J. Plant Physiol., 2011, vol. 58, p. 271. https://doi.org/10.1134/S1021443711020178

    Article  CAS  Google Scholar 

  17. Nesterenko, T.V., Shikhov, V.N., and Tikhomirov, A.A., Chlorophyll fluorescence as an indicator of age-dependent changes in photosynthetic apparatus of wheat leaves, Russ. J. Plant Physiol., 2015, vol. 62, p. 307. https://doi.org/10.1134/S1021443715020144

    Article  CAS  Google Scholar 

  18. Nesterenko, T.V. and Sid’ko, F.Ya., Quantitative description of slow induction of chlorophyll fluorescence in ontogenesis of higher plant leaves, Fiziol. Rast., 1993, vol. 40, p. 10.

    CAS  Google Scholar 

  19. Tikhomirov, A.A. and Sid’ko, F.Ya., Photosynthesis and structure of radish and wheat canopies as affected by radiation of different energy and spectral composition, Photosynthetica, 1982, vol. 16, p. 191.

    CAS  Google Scholar 

  20. Tikhomirov, A.A., Intracanopy lighting in phytocenoses and photobiological efficiency of radiation in photoculture conditions, Light Eng., 2021, vol. 29, no. 2, p. 2.

    Google Scholar 

  21. Polyakova, I.B., Karavaev, V.A., Solntsev, M.K., and Chechulina, A.A., Luminescence indices of different parts of the wheat leaf during ontogeny, Biophysics (Moscow), 2003, vol. 48, p. 1025.

    Google Scholar 

  22. Lichtenthaler, H., Buschmann, C., and Knapp, M., Measurement of chlorophyll fluorescence kinetics (Kautsky effect) and the chlorophyll fluorescence decrease ratio (RFd-values) with the PAM-fluorometer, in Analytical Methods in Plant Stress Biology, Filek, M., Biesaga-Kocielniak, J., and Marciska, I., Eds., Krakow: Franciszek Gorski Inst. Plant Physiol., Pol. Acad. Sci., 2004, p. 93.

    Google Scholar 

  23. Ushakov, I.B., Shtemberg, A.S., and Shafirkin, A.V., Reaktivnost’ i rezistentnost’ organizma mlekopitayushchikh (Reactivity and Resistance of the Organism of Mammals), Moscow: Nauka, 2007.

  24. Nesterenko, T.V., Tikhomirov, A.A., and Shikhov, V.N., Influence of excitation light intensity and leaf age on the slow chlorophyll fluorescence transient in radish, Biophysics (Moscow), 2012, vol. 57, p. 464. https://doi.org/10.1134/S0006350912040136

    Article  CAS  Google Scholar 

  25. Nesterenko, T.V., Shikhov, V.N., and Tikhomirov, A.A., Light dependence of slow chlorophyll fluorescence induction in the course of wheat leaf ontogeny, Dokl. Biochem. Biophys., 2014, vol. 454, p. 38. https://doi.org/10.1134/S1607672914010116

    Article  CAS  PubMed  Google Scholar 

  26. Bukhov, N.G., Leaf senescence: an evaluation of limiting steps in photosynthesis by means of chlorophyll fluorescence–quenching coefficients and P700 redox changes in leaves, Fiziol. Rast., 1997, vol. 44, p. 352.

    Google Scholar 

  27. Nesterenko, T.V. and Sid’ko, F.Ya., Induction of chlorophyll a fluorescence in ontogenesis of a wheat flag leaf, in Biofizicheskie metody issledovaniya ekosistem (Biophysical Study Methods of Ecosystems), Terskov, I.A., Ed., Novosibirsk: Nauka, 1984, p. 83.

  28. Tooming, H., Mathematical model of plant photosynthesis considering adaptation, Photosynthetica, 1967, vol. 1, p. 233.

    Google Scholar 

  29. Tooming, Kh.G., Ekologicheskie printsipy maksimal’noi produktivnosti posevov (Ecological Principles for Maximum Crop Yield), Leningrad: Gidrometeoizdat, 1984.

  30. Nesterenko, T.V., Tikhomirov, A.A., and Shikhov, V.N., Ontogenetic approach to the assessment of plant resistance to prolonged stress using chlorophyll fluorescence induction method, Photosynthetica, 2006, vol. 44, p. 321. https://doi.org/10.1007/s11099-006-0031-8

    Article  CAS  Google Scholar 

  31. Drozdova, I.S., Bondar, V.V., and Bukhov, N.G., Effect of leaf aging on induction transitions of variable of chlorophyll fluorescence. The content of ATP and metabolites of the Calvin cycle, Fiziol. Rast., 1992, vol. 39, p. 635.

    CAS  Google Scholar 

  32. Klimov, S.V., Correlation between CO2 assimilation and delayed fluorescence during their simultaneous registration from the leaf surface, Fiziol. Rast., 1988, vol. 35, p. 31.

    CAS  Google Scholar 

  33. Li, G.L., Wu, H.X., Sun, Y.Q., and Zhang, S.Y., Response of chlorophyll fluorescence parameters to drought stress in sugar beet seedlings, Russ. J. Plant Physiol., 2013, vol. 60, p. 337. https://doi.org/10.1134/S1021443713020155

    Article  CAS  Google Scholar 

  34. Shikhov, V.N., Nesterenko, T.V., and Tikhomirov, A.A., Effect of light intensity on chlorophyll fluorescence in wheat leaves: application of PAM-fluorometry, Russ. J. Plant Physiol., 2016, vol. 63, p. 417. https://doi.org/10.1134/S1021443716030134

    Article  CAS  Google Scholar 

  35. Murei, I.A. and Rakhmankulova, Z.F., Relation between photosynthesis and respiration components in sugar beet during the vegetative growth phase, Fiziol. Rast., 1990, vol. 37, p. 462.

    Google Scholar 

  36. Gamalei, Yu.V., Life span of chloroplasts in mesophyll cells, Tsitologiya, 1975, vol. 17, p. 1243.

    Google Scholar 

  37. Shabala, S.N. and Voinov, O.A., Dynamics of physiological characteristics of plants as an element of ecological monitoring system, Usp. Sovrem. Biol., 1994, vol. 114, p. 144.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Institute of Biophysics, Federal Research Center, Siberian Branch, Russian Academy of Sciences, Krasnoyarsk, Russia

    T. V. Nesterenko, A. A. Tikhomirov & V. N. Shikhov

  2. Siberian State University, Krasnoyarsk, Russia

    A. A. Tikhomirov

Authors
  1. T. V. Nesterenko
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. A. A. Tikhomirov
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. V. N. Shikhov
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to V. N. Shikhov.

Ethics declarations

Conflict of interests. The authors declare that they have no conflicts of interest.

Statement on the welfare of humans or animals. This article does not contain any studies involving humans or animals performed by any of the authors.

Additional information

Translated by N. Balakshina

Abbreviations: IDA—irradiation density of adaptation; CFI—chlorophyll fluorescence induction; PAR—photosynthetically active radiation; PSA—photosynthetic apparatus.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Nesterenko, T.V., Tikhomirov, A.A. & Shikhov, V.N. Physiological (Integral) Approach to the Use of Chlorophyll Fluorescence Parameters of Plant Leaves. Russ J Plant Physiol 69, 53 (2022). https://doi.org/10.1134/S1021443722030116

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • DOI: https://doi.org/10.1134/S1021443722030116

Keywords:

Search

Navigation