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Effect of Lactone- and Ketone-Containing Brassinosteroids on Photosynthetic Activity of Barley Leaves during Aging

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Abstract

A comparison was made of the physiological activity of brassinosteroids (BS) of different chemical structures—lactone-containing 24-epibrassinolide (EBL) and ketone-containing brassinosteroid 24-epicastasterone (ECS)—during dark aging of cut barley (Hordeum vulgare L.) leaves. The functional state of the photosynthetic apparatus, the amount of photosynthetic pigments, the content of lipid peroxidation products, and gene expression of HvS40 (senescence marker) were assessed. The separated barley leaves were incubated on solutions of EBL and ECS in the concentration range of 0.01–1 µM in Petri dishes on filter paper. Synthetic cytokinin 6-benzylaminopurine (BAP) at a 10 μM concentration was used as a positive control, and distilled water was used as a negative control. Petri dishes with leaves were kept in the dark for 5 days. Measurements of the analyzed parameters were carried out on the first, third, and fifth days from the beginning of incubation, mainly in the middle part of the leaf plate. ECS at a concentration of 1 μM reduced oxidative stress by the fifth day of incubation to a greater extent than EBL at a similar concentration. Regarding maintenance of chlorophyll a (Chl a) levels, 24-epicastasterone (1 μM) was more effective than 24-epibrassinolide. It was shown that exogenous brassinosteroids showed a protective effect on the main photochemical processes of the second photosystem during aging of barley leaves. Moreover, when assessing the coefficient of nonphotochemical quenching (qN), indicators of regulated (Y(NPQ)) and nonregulated (Y(NO)) energy dissipation, the ECS showed a more pronounced protective effect for the second photosystem compared to EBL. It was found that treatment with brassinosteroids reduced the level of gene expression of HvS40, a senescence marker on the fifth day of incubation, which was especially characteristic for the ketone-containing brassinosteroid. Thus, it can be concluded that the ketone-containing brassinosteroid not only exhibits high biological activity but, in terms of a number of parameters, is also more effective than the lactone-containing brassinosteroid in reducing the damage caused by aging.

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Funding

This work was financially supported by the Russian Foundation for Basic Research, project no. 19-34-50076 mol_nr.

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Authors and Affiliations

  1. National Research Tomsk State University, Tomsk, Russia

    I. S. Kovtun, N. E. Kukharenko & M. V. Efimova

  2. Timiryazev Institute of Plant Physiology, Russian Academy of Sciences, Moscow, Russia

    V. V. Kusnetsov

  3. Institute of Bioorganic Chemistry, National Academy of Sciences of Belarus, Minsk, Republic of Belarus

    V. A. Khripach

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  1. I. S. Kovtun
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  2. N. E. Kukharenko
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Correspondence to I. S. Kovtun.

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Abbreviations: BL—brassinolide; BS—brassinosteroids; EBL—24-epibrassinolide; ECS—24-epicastasterone; FLC—chlorophyll fluorescence; TBARS—thiobarbituric acid reactive substances.

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Kovtun, I.S., Kukharenko, N.E., Kusnetsov, V.V. et al. Effect of Lactone- and Ketone-Containing Brassinosteroids on Photosynthetic Activity of Barley Leaves during Aging. Russ J Plant Physiol 68, 440–450 (2021). https://doi.org/10.1134/S1021443721030080

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