Abstract
New parameters are proposed that allow reliable calculation of fixed hydrophilicity values for different classes of lipids over the widest possible range, based on the elution power of solvents and using two compounds at the boundaries of the range as standards. The values of relative hydrophilicity are calculated from the values of relative chromatographic mobility of these types of compounds. It is established that the levels of hydrophilicity of different classes of lipids relative to the selected hexadecane–glycerol pair do not depend on the composition of the different mobile phases used in either planar or column types of liquid chromatography for the separation of complex lipid mixtures.
Similar content being viewed by others
References
V. D. Tsydendambaev, W. W. Christie, E. Y. Brechany, and A. G. Vereshchagin, Phytochemistry 65, 2695 (2004).
A. G. Vereshchagin, Izv. Akad. Nauk SSSR, Ser. Biol., No. 1, 54 (1981).
K. Kaczmarski, W. Prus, and T. Kowalska, J. Chromatogr., A 869, 57 (2000).
M. Czok and G. Guiochon, Anal. Chem. 62, 189 (1990).
B. G. Belen’kii and L. Z. Vilenchik, Polymer Chromatography (Khimiya, Moscow, 1979) [in Russian].
S. D. Kolev, Anal. Chim. Acta 229, 183 (1990).
K. I. Sakodynskii, V. V. Brazhnikov, S. A. Volkov, et al., Analytical Chromatography (Khimiya, Moscow, 1993), pp. 294, 298 [in Russian].
O. B. Rudakov, Kondens. Sredy Mezhfazn. Granitsy 4, 140 (2002).
O. B. Rudakov and L. V. Rudakova, Sorbtsion. Khromatogr. Protsessy 12, 231 (2012).
J. H. Dhont, C. Vinkenborg, H. Compaan, et al., J. Chromatogr. 47, 376 (1970).
R. A. Zeeuw, H. Compaan, F. J. Ritter, et al., J. Chromatogr. 47, 382 (1970).
M. D. van Wendel de Joode, H. Hindriks, and J. Lakeman, J. Chromatogr. 170, 412 (1979).
M. Kates, Techniques of Lipidology (Elsevier, Amsterdam, 1972).
P. C. Sadek, The HPLC Solvents Guide (Wiley, New York, 2012; Binom. Laboratoriya Znanii, Moscow, 2014).
L. R. Snyder, P. W. Carr, and S. C. Rutan, J. Chromatogr., A 656, 537 (1993).
M. J. Kamlet, J.-L. M. Abboud, M. H. Abraham, and R. W. Taft, Org. Chem. 48, 2877 (1983).
M. Chastrette, M. Rajzmann, M. Chanon, and K. F. Purcell, J. Am. Chem. Soc. 107, 1 (1985).
Author information
Authors and Affiliations
Corresponding author
Additional information
Original Russian Text © V.P. Pchelkin, 2016, published in Zhurnal Fizicheskoi Khimii, 2016, Vol. 90, No. 9, pp. 1409–1413.
Rights and permissions
About this article
Cite this article
Pchelkin, V.P. Elution power of a solvent as a criterion of relative lipid polarity. Russ. J. Phys. Chem. 90, 1885–1888 (2016). https://doi.org/10.1134/S0036024416090235
Received:
Published:
Issue Date:
DOI: https://doi.org/10.1134/S0036024416090235