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Optimization of the Method for Determining the Motility Characteristics of Fish spermatozoa Using ImageJ Software and Excel Macros

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Abstract

A method for computer-based determination of the motility characteristics of fish spermatozoa using ImageJ software and a number of original Excel macros that help optimizing the processing of the data obtained using the wrMTrck_Batch plugin are described. The described method has been tested on sperm of the Black Sea turbot Scophthalmus maeoticus.

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Authors and Affiliations

  1. Kovalevsky Institute of Marine Biological Research, Russian Academy of Sciences (IMBR RAS), 299011, Sevastopol, Russia

    Iu. S. Baiandina & A. N. Khanaychenko

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  1. Iu. S. Baiandina
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  2. A. N. Khanaychenko
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Correspondence to Iu. S. Baiandina.

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Translated by D. Martynova

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Baiandina, I.S., Khanaychenko, A.N. Optimization of the Method for Determining the Motility Characteristics of Fish spermatozoa Using ImageJ Software and Excel Macros. J. Ichthyol. 59, 127–130 (2019). https://doi.org/10.1134/S0032945219010016

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  • DOI: https://doi.org/10.1134/S0032945219010016

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