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RNA silencing of the anionic peroxidase gene impairs potato plant resistance to Phytophthora infestans (Mont.) de Bary

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Abstract

Potato Solanum tuberosum L. plants expressing an antisense M21334 fragment were obtained by agrobacterial transformation. A manifold decrease in activity of anionic isoperoxidase with pI ∼ 3.5 in the transformed plants demonstrated that the enzyme is encoded by M21334. The transformed plants showed a decrease in lignin accumulation and a dramatically lower resistance to the late blight agent Phytophthora infestans, implicating the enzyme in the response to P. infestans infection.

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Abbreviations

MS:

Murashige and Skoog medium

LB:

Luria-Bertani medium

PB:

phosphate buffer

pI:

isoelectric point

DAB:

3,3-diaminobenzidine

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Authors and Affiliations

  1. Institute of Biochemistry and Genetics, Ufa Research Center, Russian Academy of Sciences, Ufa, 450054, Russia

    A. V. Sorokan, B. R. Kuluev, G. F. Burkhanova & I. V. Maksimov

Authors
  1. A. V. Sorokan
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  2. B. R. Kuluev
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  3. G. F. Burkhanova
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  4. I. V. Maksimov
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Correspondence to I. V. Maksimov.

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Original Russian Text © A.V. Sorokan, B.R. Kuluev, G.F. Burkhanova, I.V. Maksimov, 2014, published in Molekulyarnaya Biologiya, 2014, Vol. 48, No. 5, pp. 814–823.

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Sorokan, A.V., Kuluev, B.R., Burkhanova, G.F. et al. RNA silencing of the anionic peroxidase gene impairs potato plant resistance to Phytophthora infestans (Mont.) de Bary. Mol Biol 48, 709–717 (2014). https://doi.org/10.1134/S0026893314050124

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  • DOI: https://doi.org/10.1134/S0026893314050124

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