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Characterization of Rhodotorula mucilaginosa RSRod01 Isolated from Trifolium sp. Root Nodules Growing at a Pb-Zn Mine Site

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Abstract

New advance in plant–microorganism interactions research revealed the ability of plants to affect the composition of root-associated microbial communities, selecting for the microorganisms required for their growth in different ecosystems under stress conditions. Yeasts can play important roles in promoting plant growth; however, little information is available in this regard. In this study, we evaluated the ability of Rhodotorula mucilaginosa RSRod01 strain isolated from the root nodules of Trifolium sp. collected from a Pb-Zn mine soil to promote plant growth in vitro by producing a siderophore and extracellular enzymes, and exhibiting antifungal activity, as well as its tolerance to heavy metals and salinity. The results showed that the isolate possessed important plant growth-promoting (PGP) traits, including the capacity to grow at 4% salt concentration and resistance to high levels of heavy metals. Resistance to heavy metals decreased in the following row: Pb2+ > Cu2+ > Co2+ > Zn2+ = Ni2+ > Cd2+> Cr6+.

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ACKNOWLEDGMENTS

This work was supported by the General Directorate for Scientific Research and Technological development (GDSRTD) (Algeria). We are grateful to Benjamin Gourion for hosting Sarah Rahal in Laboratory Des Interactions plants Micro-organismes (LIPM, Toulouse, France), for the help in the experiments and his extremely useful advice throughout this research. The authors would like to thank also Claire Benezech for her help and advice.

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  1. Laboratory of Applied Biochemistry and Microbiology (LABM), Faculty of Sciences, Department of Biochemistry, University Badji Mokhtar Annaba, 23000, Annaba, Algeria

    S. Rahal, B. Menaa & D. Chekireb

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Rahal, S., Menaa, B. & Chekireb, D. Characterization of Rhodotorula mucilaginosa RSRod01 Isolated from Trifolium sp. Root Nodules Growing at a Pb-Zn Mine Site. Microbiology 92, 860–867 (2023). https://doi.org/10.1134/S0026261723600891

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