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Complete Genome Sequence of an Uncultured Bacterium of the Candidate Phylum Bipolaricaulota

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Abstract

Bacteria of the candidate phylum Bipolaricaulota, previously known as OP1 and Acetothermia, have been identified in various terrestrial and marine ecosystems as a result of molecular analysis of microbial communities. To date, none of the members of Bipolaricaulota has been isolated in a pure culture. We assembled the complete closed genome sequence of the bacterium of this phylum from the metagenome of a subsurface thermal aquifer and used the genomic data to analyze the phylogenetic position and metabolic potential of this bacterium. Analysis of the genome of this bacterium, designated Ch78, revealed various transporters of sugars and peptides and limited capacities for the extracellular hydrolysis of polysaccharides. Reconstruction of the central metabolic pathways suggested that the bacterium Ch78 is an anaerobic organotroph capable of fermenting sugars and proteinaceous substrates. Known pathways of aerobic and anaerobic respiration, as well as of autotrophic carbon fixation, were not found in the Ch78 genome. In the subsurface aquifer Bipolaricaulota probably consume low-molecular weight organic compounds and produce hydrogen and acetate. Based on the results of phylogenetic and genomic analysis, classification of the novel bacterium as “Candidatus Bipolaricaulis sibiricus” is proposed.

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ACKNOWLEDGMENTS

The work was supported by the Russian Foundation for Basic Research, project no. 16-34-60124 and by the Ministry of Science and Higher Education of the Russian Federation.

The work was carried out using the scientific equipment of the Core Research Facility “Bioengineering” (Research Center of Biotechnology Russian Academy of Sciences).

Funding

The work was supported by the Russian Foundation for Basic Research, project no. 16-34-60124 and by the Ministry of Science and Higher Education of the Russian Federation.

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Authors and Affiliations

  1. Institute of Bioengineering, Research Center of Biotechnology, Russian Academy of Sciences, 119071, Moscow, Russia

    V. V. Kadnikov, A. V. Mardanov, A. V. Beletsky & N. V. Ravin

  2. Faculty of Biology, Moscow State University, 119192, Moscow, Russia

    V. V. Kadnikov & N. V. Ravin

  3. Tomsk State University, 634050, Tomsk, Russia

    Y. A. Frank & O. V. Karnachuk

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  1. V. V. Kadnikov
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Correspondence to V. V. Kadnikov.

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The authors declare that they have no conflict of interest. This article does not contain any studies involving animals or human participants performed by any of the authors.

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Translated by P. Sigalevich

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Kadnikov, V.V., Mardanov, A.V., Beletsky, A.V. et al. Complete Genome Sequence of an Uncultured Bacterium of the Candidate Phylum Bipolaricaulota. Microbiology 88, 461–468 (2019). https://doi.org/10.1134/S0026261719040064

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