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Role of the microbial community in formation of speleothem (moonmilk) in the Snezhnaya carst cave (abkhazia)

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Abstract

The results of investigation of speleothem (moonmilk) from the Snezhnaya cave (West Caucasus, Abkhazia) are reported. The structure of microbial complexes from moonmilk was investigated by quantitative PCR; strains of culturable microorganisms were isolated and their preferred temperature and carbon sources were determined. Among eubacteria, ~34% belonged to the iron bacteria (Rhodoferax). Most bacterial strains were shown to be facultative psychrophiles with the maximum growth rate at 4°C. The microstructure and elemental composition of moonmilk were investigated using a scanning electron microscope (EVO-40HV, Carl Zeiss) and silicon drift X-ray detector X-MAX 80mm2. The visually plastic and homogeneous mass of moonmilk was shown to be highly heterogeneous, containing various microstructures. The elemental composition of some nanostructures depended on the structure of bacterial biofilms. Some loci of the biofilm were found to contain up to 46% (wt/wt) of iron oxides. High calcium content, up to 61.5% (wt/wt), was found only in cubic crystalline structures which were not involved in microbiological processes.

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References

  • Barton, H.A. and Northup, D.E., Geomicrobiology in cave environments: past, current and future perspectives, J. Cave Karst Stud., 2007, vol. 69, pp. 163–178.

    Google Scholar 

  • Bazarova, E.P., Mazina, S.E., and Khodyreva, E.V., Mineralogical researches at Snezhnaya-Mezhennogo-Illuzia cave system (Bzibskiy ridge, Western Caucasus): preliminary results and directions for future researches, Speleol. Karstol., 2013, no. 10, pp. 76–85.

    Google Scholar 

  • Borsato, A., Frisia, S., Jones, B., and Van der Borg, K., Calcite Moonmilk: crystal morphology and environment of formation in caves in the Italian Alps, J. Sediment. Res., 2000, vol. 70, pp. 1179–1190.

    Article  Google Scholar 

  • Braissant, O., Bindschedler, S., Daniels, A.U., Verrecchia, E.P., and Cailleau, G., Microbiological activities in moonmilk monitored using isothermal microcalorimetry (Cave of Vers chez le Brandt, Neuchatel, Switzerland), J. Cave Karst Stud., 2012, vol. 74, pp. 116–126.

    Article  CAS  Google Scholar 

  • Cacchio, P., Ferrini, G., Ercole, C., Del Gallo, M., and Lepidi, A., Biogenicity and characterization of moonmilk in the Grotta Nera (Majella National Park, Abruzzi, central Italy), J. Cave Karst Stud., 2014, vol. 76, pp. 88–103.

    Article  Google Scholar 

  • Chirienco, M., Calcite moonmilk in the Humpleu Cave System (Romania): The relationship between crystal morphology and cave top climate, Cave Karst Sci., 2002, vol. 29, pp. 101–104.

    Google Scholar 

  • Cosmidis, J., Benzerara, K., Gheerbrant, E., Estève, I., Bouya, B., and Amaghzaz, M., Nanometer-scale characterization of exceptionally preserved bacterial fossils in Paleocene phosphorites from Ouled Abdoun (Morocco), Geobiology, 2013, vol. 11, pp. 139–153.

    Article  CAS  PubMed  Google Scholar 

  • Dubinina, G.A. and Sorokina, A.Yu., Neutrophilic lithotrophic iron-oxidizing prokaryotes and their role in the biogeochemical processes of the iron cycle, Microbiology (Moscow). 2014, vol. 83, no. 1–2, pp. 1–15.

    Article  CAS  Google Scholar 

  • Fernandez-Cortes, A., Cuezva, S., Sanchez-Moral, S., Cañaveras, J.C., Porca, E., Jurado, V., Martin-Sanchez, P.M., and Saiz-Jimenez, C., Detection of human-induced environmental disturbances in a show cave, Environ. Sci. Pollut. Res., 2011, vol. 18, pp. 1037–1045.

    Article  CAS  Google Scholar 

  • Finnerant, K.T., Johnsen, C.V., and Lovley D.R., Rhodoferax ferrireducens sp. nov., a psychrotolerant, facultatively anaerobic bacterium that oxidizes acetate with the reduction of Fe(III), Int. J. Syst. Evol. Microbiol., 2003, vol. 53, pp. 669–673.

    Article  Google Scholar 

  • Hill, C.A. and Forti, P., Cave mineralogy and the NSS: past, present, future, J. Cave Karst Stud., 2007, vol. 69, pp. 35–45.

    Google Scholar 

  • Jurado, V., Laiz, L., Rodriguez-Nava, V., Boiron, P., Hermosin, H., Sanchez-Moral, S., and Saiz-Jimenez, C., Pathogenic and opportunistic microorganisms in caves, Int. J. Speleol., 2010, vol. 39, pp. 15–24.

    Article  Google Scholar 

  • Karavaiko G.I., Dubinina G.A., Kondrat’eva T.F., Lithotrophic microorganisms of the oxidative cycles of sulfur and iron, Microbiology (Moscow). 2006, vol. 75, no. 5, pp. 512–546.

    Article  CAS  Google Scholar 

  • Khizhnyak, S.V., Tausheva, I.V., Berezikova, A.A., Nesterenko, E.V., and Rogozin, D.Yu., Psychrophilic and psychrotolerant heterotrophic microorganisms of Middle Siberian karst cavities, Russ. J. Ecol., 2003, vol. 34, no. 4, pp. 231–236.

    Article  Google Scholar 

  • Kondrat’eva, L.M. and Litvinenko, Z.N., In vitro biofilm formation by groundwater microbial complexes, Biotekhnologiya, 2014, no. 3, pp. 73–82.

    Google Scholar 

  • Kubista, M., Andrade, J.M., Bengtsson, M., Forootan, A., Jonake, J., Lind, K., Sindelka, R., Sjoback, R., Sjogreen, B., Strombom, L., Stahlberg, A., and Zoric, N., The real-time polymerase chain reaction, Mol. Aspects Medicine, 2006, vol. 27, pp. 95–125.

    Article  CAS  Google Scholar 

  • Kuzmina, L.Yu., Galimzianova, N.F., Abdullin, Sh.R., and Ryabova, A.S., Microbiota of the Kinderlinskaya Cave (South Urals, Russia)}, Microbiology (Moscow). 2012, vol. 81, no. 2, pp. 251–259.

    Article  CAS  Google Scholar 

  • Kuznetsiv, S.I. and Karavaiko, G.I., Role of microorganisms in the geochemical processes of element transfer in Earth crust, Trudy biogeokhimicheskoi laboratorii (Proceedings of the Biogeochemical Laboratory), Moscow: Nauka, 1979, pp. 91–100.

    Google Scholar 

  • Lyudkovskii, G.V., Mavlyudov, B.R., Morozov, A.I., Nemchenko, T.A., and Usikov, D.A., Investigation of the Snezhnaya cave, the deepest karst cave in the USSR, DAN SSSR, 1981, vol. 259, no. 2, pp. 437–442.

    Google Scholar 

  • Maksimovich, N.G. and Khrumchik, V.T., Effect of microorganisms on the mineral composition and properties of soils, Vestn. Perm Univ. Ser. Geol., 2012, no. 3, pp. 47–54.

    Google Scholar 

  • Mazina, S.E. and Semikolennykh, A.A., Different forms of moonmilk in Russian caves in the light of the genesis problem, Peshchery, 2010, no. 33, pp. 34–44.

    Google Scholar 

  • Mazina, S.E., Bazarova, E.P., and Kotsevova, A.A., Sanitary important microbiota of the Snezhnaya-Mezhennogo-Illuzia cave system, Fundament. Issled., 2015, no. 2, pp. 5808–5814.

    Google Scholar 

  • Mitchell, A.C. and Ferris F.G., The influence of Bacillus pasteurii on the nucleation and growth of calcium carbonate, Geomicrobiol. J., 2006, vol. 23. P. 213–226.

    Article  CAS  Google Scholar 

  • Northup, D.E. and Lavoie, K.H., Geomicrobiology of Caves: A Review, Geomicrobiol. J., 2001, vol. 18, pp. 199–222.

    Article  CAS  Google Scholar 

  • Ovsyankina (Vorob’eva), S.V., Ilients, I.R., and Khizhnyak, S.V., Psychrophilic and psychrotolerant microorganisms of the Sarma cave, Vestn. KrasGAU, 2011, no. 7, pp. 112–118.

  • Perry, T.D., Duckworth, O.W., McNamara, C.J., Martin, S.T. and Mitchell, R., Effects of the biologically produced polymer alginic acid on macroscopic and microscopic calcite dissolution rates, Environ. Sci. Technol., 2004, vol. 38, pp. 3040–3046.

    Article  CAS  PubMed  Google Scholar 

  • Portillo, M.C. and Gonzales, J.M., Moonmilk deposits originate from specific bacterial communities in Altamira Cave (Spain), Microb. Ecol., 2011, vol. 61, pp. 182–189.

    Article  PubMed  Google Scholar 

  • Semikolennykh, A.A., Ivanova, A.E., Gorlenko, M.V., and Dobrovol’skaya, T.G., Ecology of microbial communities of carstogenic landscapes of the White Sea-Kuloi plateau (Arkhangelsk oblast), Peshchery (Caves), Sidorov, L.P., Ed., Perm, 2008, vol. 31, pp. 120–143.

    Google Scholar 

  • Shvartsev, S.L., Ryzhenko, B.N., and Alekseev, V.A., Geologicheskaya evolyutsiya i samoorganizatsiya sistemy bodaporoda (Geological Evolution and self-organization of the water–rock system), Novosibirsk: SO RAS, 2007. 389 p.

    Google Scholar 

  • Smith, K.S. and Ferry, J.G., Prokaryotic carbonic anhydrases, FEMS Microbiol. Rev., 2000, vol. 24, pp. 335–366.

    Article  CAS  PubMed  Google Scholar 

  • Summers, En.A., Paoletti, M.G., Beggio, M., Dorigo, L., Pamio, A., Gomiero, T., Furlan, C., Brilli, M., Dreon, A.L., Bertoni, R., and Squartini, A., Comparative microbial community composition from secondary carbonate (moonmilk) deposits: implications for the Cansiliella servadeii cave hygropetric food web, Int. J. Speleol., 2013, vol. 42, no. 3, pp. 181–192.

    Article  Google Scholar 

  • Vakhrushev, B.A., Dublyanskii, B.N., and Amelichev, G.N., Bzipi Range Karst. Western Caucasus, Moscow: RUDN, 2001.

    Google Scholar 

  • Zavarzin, G.A. and Kolotilova, N.N., Vvedenie v prirodovedcheskoi mikrobiologii (Introduction to Natural Science Microbiology), Moscow: Universitet, 2001.

    Google Scholar 

  • Zavarzin, G.A., Evolution of prokaryotic biosphere: Microbes in turnover of life: 120 years later, Chtenie im. S.N. Vinogradskogo (Winogradsky Readings), Kolotilova, N.N., Ed., Moscow: MAKS Press, 2011, p. 144.

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Authors and Affiliations

  1. Institute of Water and Ecological Problems, Far Eastern Branch, Russian Academy of Sciences, Khabarovsk, Russia

    L. M. Kondratyeva, O. S. Polevskaya & Z. N. Litvinenko

  2. Yu. A. Kosygin Institute of Tectonics and Geophysics, Far Eastern Branch, Russian Academy of Sciences, Khabarovsk, Russia

    E. M. Golubeva & N. S. Konovalova

Authors
  1. L. M. Kondratyeva
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  2. O. S. Polevskaya
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  3. Z. N. Litvinenko
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  4. E. M. Golubeva
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  5. N. S. Konovalova
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Correspondence to Z. N. Litvinenko.

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Original Russian Text © L.M. Kondratyeva, O.S. Polevskaya, Z.N. Litvinenko, E.M. Golubeva, N.S. Konovalova, 2016, published in Mikrobiologiya, 2016, Vol. 85, No. 5, pp. 598–608.

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Kondratyeva, L.M., Polevskaya, O.S., Litvinenko, Z.N. et al. Role of the microbial community in formation of speleothem (moonmilk) in the Snezhnaya carst cave (abkhazia). Microbiology 85, 629–637 (2016). https://doi.org/10.1134/S002626171605009X

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