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Characterization of extracellular yeast peptide factors and their stress-protective effect on probiotic lactic acid bacteria

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Abstract

Protective effect of the extracellular peptide fraction (reactivating factors, RF) produced by yeasts of various taxonomic groups (Saccharomyces cerevisiae, Kluyveromyces lactis, Candida utilis, and Yarrowia lipolytica) on probiotic lactic acid bacteria (LAB) Lactobacillus casei, L. acidophilus, and L. reuteri under bile salt (BS)-induced stress was shown. RF of all yeasts were shown to be of peptide nature; the active component of the S. cerevisiae RF was identified as a combination of low-molecular polypeptides with molecular masses of 0.6 to 1.5 kDa. The protective and reactivating effects of the yeast factors were not species-specific and were similar to those of the Luteococcus japonicus subsp. casei RF. In BS-treated cells of the tester bacteria, a protective effect was observed after 10-min preincubation of the LAB cell suspension with yeast RF: the number of surviving cells (CFU) was 2 to 4.5 times higher than in the control. The reactivating effect was observed when RF was added to LAB cell suspensions not later than 15 min after stress treatment. It was less pronounced than the protector effect, with the CFU number 1 to 3 times that of the control. Both the protector and the reactivating effects were most pronounced in the S. cerevisiae and decreased in the row C. utilis > K. lactis > Y. lipolytica. The efficiency of protective action of yeast RF was found to depend on the properties of recipient LAB cells, with the L. casei strain being most sensitive to BS treatment. In both variants, the highest protective effect of RF (increase in the CFU number) was observed for L. acidophilus, while the least pronounced one was observed for L. casei. The reasons for application of the LAB strains combining high stress resistance and high response to stress-protecting metabolites, including RF factors, as probiotics, is discussed.

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Authors and Affiliations

  1. Department of Biology, Lomonosov Moscow State University, Moscow, Russia

    L. I. Vorob’eva, E. Yu. Khodzhaev, T. A. Cherdyntseva & A. I. Netrusov

  2. Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia

    E. A. Rogozhin

Authors
  1. L. I. Vorob’eva
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  2. E. Yu. Khodzhaev
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  3. E. A. Rogozhin
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  4. T. A. Cherdyntseva
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  5. A. I. Netrusov
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Correspondence to L. I. Vorob’eva.

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Original Russian Text © L.I. Vorob’eva, E.Yu. Khodzhaev, E.A. Rogozhin, T.A. Cherdyntseva, A.I. Netrusov, 2016, published in Mikrobiologiya, 2016, Vol. 85, No. 4, pp. 393–402.

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Vorob’eva, L.I., Khodzhaev, E.Y., Rogozhin, E.A. et al. Characterization of extracellular yeast peptide factors and their stress-protective effect on probiotic lactic acid bacteria. Microbiology 85, 411–419 (2016). https://doi.org/10.1134/S0026261716040160

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  • DOI: https://doi.org/10.1134/S0026261716040160

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