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The Effects of Phenanthrene on the Electrical Activity in the Heart of Shorthorn Sculpin (Myoxocephalus scorpio)

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Abstract

Phenanthrene (Phen) is one of the widespread species of polyaromatic hydrocarbons, a tricyclic compound, component of crude oil and diesel fuel. Earlier studies have shown its negative effects on fish heart function that may explain the toxicity of this pollutant. The present study aimed to elucidate the cardiac electrophysiological effects of Phen in shorthorn sculpin, the widespread and common fish of the Arctic region using the patch-clamp technique. We have found pronounced effects of Phen on the duration and upstroke velocity of action potentials (APs) in sculpin ventricular myocytes. 1 µM Phen produced almost two-fold prolongation of AP duration measured at 90% repolarization level and significant reduction of maximal upstroke velocity of AP. These effects are attributed to suppression of K+ rapid delayed rectifier current IKr (IC50 = 144 nM) and fast Na+ current INa (IC50 = 5.85 µM). Resting membrane potential and K+ inward rectifier current IK1 were not affected by Phen even in the maximal concentration of 30 µM. Thus, low concentrations of Phen induce changes in cellular electrical activity of sculpin ventricular myocytes which might lead to proarrhythmic effects such as slowing of repolarization and decrease in excitation conduction velocity at the tissue level.

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Funding

This work was supported by Russian Science Foundation (grant no. 22-14-00075) to DVA.

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Authors and Affiliations

  1. Department of Human and Animal Physiology, Lomonosov Moscow State University, Moscow, Russia

    T. S. Filatova, V. O. Guskova & D. V. Abramochkin

  2. Laboratory of Cardiac Electrophysiology, Chazov National Medical Research Center for Cardiology, Moscow, Russia

    T. S. Filatova

  3. Department of Physiology, Pirogov Russian National Research Medical University, Moscow, Russia

    T. S. Filatova

  4. Almazov National Medical Research Center, St. Petersburg, Russia

    V. B. Mikhailova

Authors
  1. T. S. Filatova
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  2. V. B. Mikhailova
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  3. V. O. Guskova
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  4. D. V. Abramochkin
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Correspondence to D. V. Abramochkin.

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The authors declare that they have no conflicts of interest.

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Translated by A. Polyanovsky

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Filatova, T.S., Mikhailova, V.B., Guskova, V.O. et al. The Effects of Phenanthrene on the Electrical Activity in the Heart of Shorthorn Sculpin (Myoxocephalus scorpio). J Evol Biochem Phys 58 (Suppl 1), S44–S51 (2022). https://doi.org/10.1134/S0022093022070055

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  • DOI: https://doi.org/10.1134/S0022093022070055

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