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Orexin-Immunopositive Structures in the Thalamic Reticular Nucleus

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Abstract

Orexin-A and orexin-B were immunolocalized in laboratory rodents (adult Wistar and Sprague–Dawley rats, C57Bl/6J mice) in neurons of different areas of the thalamic reticular nucleus, where the level of their immunoreactivity was significantly lower than in neurons of the hypothalamic perifornical area. In Wistar rats, orexin-immunopositive neurons in these brain structures exhibited opposite reactions after 6-h sleep deprivation and 3-h supine immobilization. During the ontogeny of Wistar rats, the thalamic orexin-immunopositive structures emerged already on E18, were still poorly developed on P14, and became definitive on P30, which coincides with the period of ultimate formation of the sleep-wake cycle in rats. These data suggest that orexins of the thalamic reticular nucleus are involved in the regulation of the sleep-wake cycle.

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Funding

This work was state budget funded (theme reg. no. ААА-А18-118012290427-7).

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  1. Sechenov Institute of Evolutionary Physiology and Biochemistry, Russian Academy of Sciences, St. Petersburg, Russia

    I. Yu. Morina & I. V. Romanova

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  1. I. Yu. Morina
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Conceptualization and experimental design (I.V.R.); conducting experiments and data collection (I.Yu.M., I.V.R.); data processing (I.Yu.M.); writing and editing the manuscript (I.V.R.).

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Correspondence to I. V. Romanova.

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Translated by A. Polyanovsky

Russian Text © The Author(s), 2022, published in Rossiiskii Fiziologicheskii Zhurnal imeni I.M. Sechenova, 2022, Vol. 108, No. 7, pp. 836–849https://doi.org/10.31857/S0869813922070056.

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Morina, I.Y., Romanova, I.V. Orexin-Immunopositive Structures in the Thalamic Reticular Nucleus. J Evol Biochem Phys 58, 1099–1109 (2022). https://doi.org/10.1134/S0022093022040135

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