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Changes in the Сontribution of IKCa Сhannels to Tone Maintenanсe and Dilation of Pial Arteries in Aging Rats after Ischemia-Reperfusion

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Abstract

Changes in the contribution of intermediate conductance Ca2+-activated potassium channels (IKCa) to basal tone maintenance and acetylcholine (ACh)-mediated dilation of pial arteries after global cerebral ischemia (“2VO + hypo” model) were studied in 18-month-old Sprague–Dawley rats. Sham-operated rats served as a control. Vascular examination was performed 7, 14 and 21 days after ischemia/reperfusion (I/R) using intravital microphotography. Changes in the contribution of IKCa channels to basal tone were assessed by the number of pial arteries constricted in response to the IKCa channel blocker clotrimazole (10–7 M, 5 min). Changes in the contribution of IKCa channels to vascular dilation was evaluated by comparing the number and degree of pial artery dilatations in response to ACh (10–7 M, 8 min) before and after clotrimazole application. It was found that I/R leads to a decrease in the number of vascular dilatations in response to ACh, which persists over the first 14 days after ischemic exposure. During this post-ischemic period, the contribution of IKCa channels to pial artery tone and ACh-mediated dilatory response is also reduced. This phenomenon is already observed 7 days following I/R, while after 14 days, IKCa channels are practically no longer involved in the dilatory response of the pial arteries to ACh. By day 21 after I/R, all the above processes recover.

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Funding

The study was supported by the State Program 47 GP “Scientific and Technological Development of the Russian Federation” (2019–2030), theme reg. no. 0134-2019-0001.

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Authors and Affiliations

  1. Pavlov Institute of Physiology, Russian Academy of Sciences, St. Petersburg, Russia

    O. P. Gorshkova & I. B. Sokolova

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  1. O. P. Gorshkova
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  2. I. B. Sokolova
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Contributions

Conceptualization and experimental design (O.P.G.); data collection (I.B.S.); data processing (O.P.G., I.B.S.); writing and editing a manuscript (O.P.G., I.B.S.).

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Correspondence to O. P. Gorshkova.

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The authors declare that they have neither evident nor potential conflict of interest related to the publication of this article.

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Translated by A. Polyanovsky

Russian Text © The Author(s), 2022, published in Rossiiskii Fiziologicheskii Zhurnal imeni I.M. Sechenova, 2022, Vol. 108, No. 5, pp. 636–648https://doi.org/10.31857/S0869813922050065.

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Gorshkova, O.P., Sokolova, I.B. Changes in the Сontribution of IKCa Сhannels to Tone Maintenanсe and Dilation of Pial Arteries in Aging Rats after Ischemia-Reperfusion. J Evol Biochem Phys 58, 815–824 (2022). https://doi.org/10.1134/S0022093022030152

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