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The Effect of Protein Overload on Reabsorption of Different Proteins in Frog Renal Tubules

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Abstract

Effects of short-term lysozyme loading and long-term administration of bovine serum albumin (BSA) on renal handling of green and yellow fluorescent proteins (GFP and YFP), as well as Alexa fluor-488-conjugated BSA (BSA-Alexa 488), were studied in grass frogs (Rana temporaria L.) and lake frogs (Rana ridibunda L.). Morphological analysis showed a more substantial decrease in the absorption capacity of proximal tubular cells after loading with lysozyme when compared with BSA loading. After BSA loading, the uptake of BSA-Alexa 488 was lower than GFP uptake, and functional changes were noted in some glomeruli. The decrease in reabsorption of GFP and YFP was accompanied by their appearance in the lumen of the distal nephron segments, as well as the formation of granular casts in the proximal tubules. The results, including the temporary nature of the observed effects, are important for comparing the reabsorption of various proteins and studying adaptive changes in nephrons at the initial stages of proteinuria.

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ACKNOWLEDGMENTS

We thank the Center for Collective Use at IEPhB RAS for providing the equipment for microscopic studies.

Funding

This study was supported by the State assignment to Sechenov Institute of Evolutionary Physiology and Biochemistry of the Russian Academy of Sciences (IEPhB RAS) (no. 075-00-776-19-02).

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  1. Sechenov Institute of Evolutionary Physiology and Biochemistry, Russian Academy of Sciences, St. Petersburg, Russia

    N. P. Prutskova & E. V. Seliverstova

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The authors contributed equally to this work.

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Correspondence to N. P. Prutskova.

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COMPLIANCE WITH ETHICAL STANDARTS

The conditions for keeping amphibians, methods of their collecting, and experimental protocols met all of the regulatory requirements for using laboratory animals approved by the Bioethics Committee at IEPhB RAS (Protocol no. 3 of 19.03.2019).

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The authors declare that they have no conflict of interest.

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The original online version of this article was revised: the issue date is not January 2020, but January 2021

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Prutskova, N.P., Seliverstova, E.V. The Effect of Protein Overload on Reabsorption of Different Proteins in Frog Renal Tubules. J Evol Biochem Phys 57, 101–114 (2021). https://doi.org/10.1134/S0022093021010105

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