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Glycogen as an Energy Storage Substrate in Fish Nucleated Erythrocytes

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Abstract

We studied the glycogen content in erythrocytes of two Black Sea cartilaginous fishes (Raja clavata, Dasyatis pastinaca) and four teleost species (Trachurus mediterraneus ponticus, Spicara flexuosa, Diplodus annularis, Scorpaena porcus). Glycogen accumulation in erythrocytes of teleost fish was about twice as high as in the ray fishes. Due to the unique ability of thornback ray R. clavata erythrocytes to well preserve their cell integrity in vitro in the cold (4°C), the dynamics of glycogen expenditure in erythrocytes was tracked over 11 days of storage until cell disintegration. Erythrocytes spent glycogen economically: during the entire storage period, only 52% of glycogen were spent. In the black scorpionfish S. porcus, erythrocytes were far less tolerant to being stored in the cold. The stability period of S. porcus erythrocytes suspended in physiological saline did not exceed two days. During this period, no significant changes in the glycogen concentration were observed.

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Funding

This work was implemented within the State assigniment no. АААА-А19-119012490045-0 “A study of fundamental physical, physiologo-biochemical, reproductive, population and behavioral characteristics of marine hydrobionts”.

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Authors and Affiliations

  1. T.I. Vyazemsky Karadag Scientific Station—Nature Reserve, Branch of A.O. Kovalevsky Institute of Biology of the Southern Seas, Russian Academy of Sciences, Feodosiya, Russia

    Yu. A. Silkin, E. N. Silkina & M. Yu. Silkin

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  1. Yu. A. Silkin
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  2. E. N. Silkina
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  3. M. Yu. Silkin
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Contributions

Yu.A. Silkin and E.N. Silkina were responsible for designing the experiments, data collection, writing and editing the manuscript. M.Yu. Silkin provided technical support during experiments, participated in data processing and discussing, as well as in graphic representation.

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Correspondence to Yu. A. Silkin.

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COMPLIANCE WITH ETHICAL STANDARDS

Experimental protocols met all applicable international, national and institutional principles of handling and using experimental animals for scientific purposes.

This study did not involve human subjects as research objects.

СONFLICT OF INTEREST

The authors declare that they have no conflict of interest.

Additional information

Translated by A. Polyanovsky

The original online version of this article was revised: the issue date is not January 2020, but January 2021

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Silkin, Y.A., Silkina, E.N. & Silkin, M.Y. Glycogen as an Energy Storage Substrate in Fish Nucleated Erythrocytes. J Evol Biochem Phys 57, 66–74 (2021). https://doi.org/10.1134/S0022093021010063

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  • DOI: https://doi.org/10.1134/S0022093021010063

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