Abstract
To evaluate the effects of photobiomodulation, we used laser devices with wavelengths of 662 nm (ALOD-01; Alcom Medica, Russia) and 808 nm (ALPH-01 Diolan; NPP VOLO, Russia) and an energy density of 1–15 J/cm2. The experiments were carried out on the infusoria Paramecium caudatum (Alveolata: Ciliophora: Oligohymenophora: Peniculia). The results were evaluated before as well as 5, 10, 30, 60 min and 24 h after irradiation. 5 min after exposure to red light (662 nm), motor activity of P. caudatum increased vs. control and then decreased within 60 minutes. 24 h after irradiation at an energy density of 15 J/cm2, the speed of P. caudatum was 52% of the initial (73% in the control) (p < 0.05). Immediately since exposure to infrared radiation (808 nm) and during the first hour, motor activity tended to decrease. 24 h later, the speed decrement was significantly less than in the control. The data obtained indicate that changes in the functional state of P. caudatum arise immediately after irradiation and persist for a long time, leading either to an increased (808 nm) or a decreased (662 nm) resistance at the same values of a radiation energy density. The peculiarities of the P. caudatum response to red vs. infrared radiation appear to be due to the differences between primary photoacceptors and appropriate signaling pathways, which come into action immediately upon their excitation.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Chow, R.T., Dose dilemmas in low level laser therapy–the effects of different paradigms and historical perspectives, Laser Therapy, 2000, vol. 13, no. 1, pp. 102–109.
Conlan, M.J., Rapley, J.W., and Cobb, C.M., Biostimulation of wound healing by low-energy laser irradiation, a review, J. Clin. Periodontol., 1996, vol. 23, no. 5, pp. 492–496.
Oron, U.Y.T., Oron, A., Mordechovitz, D., Shofti, R., Hayam, G., Dror, U., Gepstein, L., Wolf, T., Haudenschild, C., and Haim, S.B., Lowenergy laser irradiation reduces formation of scar tissue after myocardial infarction in rats and dogs, Circulation, 2001, no. 103, pp. 296–301.
De Freitas, L.F. and Hamblin, M.R., Proposed mechanisms of photobiomodulation or low-level light therapy, IEEE J. Sel. Top. Quantum Electron., 2016, vol. 22, no. 3, pp. 1–37.
Lubart, R., Lavi, R., Friedmann, H., and Rochkind, S., Photochemistry and photobiology of light absorption by living cells, Photomed. Laser Surg., 2006, no. 24, pp. 179–185.
Bolton, P., Young, S., and Dyson, M., Macrophage responsiveness to light therapy with varying power and energy densities, Laser Ther., 1991, vol. 3, no. 3, pp. 105–111.
Karu, T.I. and Kolyakov, S.F., Exact action spectra for cellular responses relevant to phototherapy, Photomed. Laser Surg., 2005, vol. 23, no. 4, pp. 355–361.
Lanzafame, R.J., Stadler, I., Kurtz, A.F., Connelly, R., Peter, T.A., Timothy, P.A., Brondon, P., and Olson, D., Reciprocity of exposure time and irradiance on energy density during photoradiation on wound healing in a murine pressure ulcer model, Lasers Surg. Med., 2007, vol. 39, no. 6, pp. 534–542.
Passarella, S. and Karu, T., Absorption of monochromatic and narrow band radiation in the visible and near IR by both mitochondrial and non-mitochondrial photoacceptors results in photobiomodulation, J. Photochem. Photobiol. B, 2014, vol. 140, pp. 344–358.
Karu, T.I., Afanasyeva, N.I. Kolyakov, S.F., Pyatibrat, L.V., and Welser, L., Changes in absorbance of monolayer of living cells induced by laser radiation at 633, 670, and 820 nm, IEEE J. Sel. Top. Quantum Electron., 2001, vol. 7, pp. 982–988.
Lane, N., Cell biology: power games, Nature, 2006, vol. 443, pp. 901–903.
Shiva, S. and Gladwin, M.T., Shining a light on tissue NO stores: near infrared release of NO from nitrite and nitrosylated hemes, J. Mol. Cell. Cardiol., 2009, vol. 46, no. 1, pp. 1–3.
Hardie, R.C., Photosensitive TRPs, Mammalian Transient Receptor Potential (TRP) Cation Channels, Nilius, B. and Flockerzi, V., Eds., Switzerland, 2014, pp. 795–826.
Mylonakis, E., Ausubel, F.M., Gilmore, M., and Casadeval, A., Recent Advances on Model Hosts, New York, 2012.
Zhou, M., Xia, H., Xu, Y., Xin, N., Liu, J., and Zhang, S., Anesthetic action of volatile anesthetics by using Paramecium as a model, J. Huazhong Univ. Sci. Technol. Med. Sci., 2012, vol. 32, no. 3, pp. 410–414.
Saib, A., Berrebbah, H., Berredjem, M., and Djebara, M.R., Cytotoxic study of three derivatives amidophosphonates on alternative cellular model: Paramecium tetraurelia, Toxicol. Res., 2014, vol. 3, no. 5, pp. 395–399.
Petrishchev, N.N., Yantareva, L.I., and Fokin, S.I., Dependence of the effect of infrared laser radiation on power flux density and functional state of a biological object (infusoria Spirostomum ambiguum), Lazer Med., 2005, vol. 9, no. 3, pp. 43–48.
Protisty: rukovodstvo po zoologii (Protists: Handbook of Zoology), Alimov, A.F., Ed., St. Petersburg, 2000.
Amaroli, A., Ravera, S., Parker, S., Panfoli, I., Benedicenti, A., and Benedicenti, S., Effect of 808 nm diode laser on swimming behavior, food vacuole formation and endogenous ATP production of Paramecium primaurelia (Protozoa), Photochem. Photobiol., 2015, vol. 91, no. 5, pp. 1150–1155.
Svidersky, V.L., Lobzin, Yu.V., Gorelkin, V.S., and Plotnikova, S.I., Motor activity of infusoria: theoretical and practical aspects, Zh. Evol. Biokhim. Fiziol., 2007, vol. 43, no. 5, pp. 379–390.
Amaroli, A., Benedicenti, A., Ferrando, S., Parker, S., Selting, W., Gallus, L., and Benedicenti, S., Photobiomodulation by infrared diode laser: effects on intracellular calcium concentration and nitric oxide production of Paramecium, Photochem. Photobiol., 2016, vol. 91, no. 5, pp. 854–862.
Tawada, K. and Oosawa, F., Responses of Paramecium to temperature change, J. Eukaryot. Microbiol., 1972, vol. 19, pp. 53–57.
Tuchin, V., Tissue optics and photonics: light–tissue interaction, J. Biomed. Photonics Eng., 2015, vol. 1, no. 2, pp. 98–135.
Malvin, G.M., Cecava, N., and Nelin, L.D., Nitric oxide production and thermoregulation in Paramecium caudatum, Acta Protozool., 2003, vol. 42, pp. 259–267.
Roszer, T., The Biology of Subcellular Nitric Oxide, The Netherlands, 2012.
Author information
Authors and Affiliations
Corresponding author
Additional information
Original Russian Text © N.N. Petrishchev, G.V. Papayan, L.V. Chistyakova, A.V. Struy, D.R. Faizullina, 2018, published in Zhurnal Evolyutsionnoi Biokhimii i Fiziologii, 2018, Vol. 54, No. 6, pp. 406–412.
Rights and permissions
About this article
Cite this article
Petrishchev, N.N., Papayan, G.V., Chistyakova, L.V. et al. Effect of Photobiomodulation by Red and Infrared Laser Radiation on Motility of Paramecium caudatum. J Evol Biochem Phys 54, 457–464 (2018). https://doi.org/10.1134/S0022093018060054
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1134/S0022093018060054