Abstract
We investigated structural changes in the Wistar rat hippocampal CA1 field and fascia dentate during the pubertal period (on P60) after perinatal hypoxic exposure as well as the distribution of GAD67-expressing neurons in these structures. It was established that in the granular layer of the fascia dentata and in the CA1 field acute perinatal hypoxia leads to irreversible homotypic abnormalities as expressed in the reduced number of neurons and their rows as well as injury of a considerable portion of cells, which exhibit the signs of chromatolysis and vacuolization of the cytoplasm. Both in experimental and control animals, GAD67-expressing neurons in the fascia dentata are scattered diffusely and share approximately the same size of their populations. In the CA1 field, immunoreactive neurons lie in the lower rows of the pyramidal layer, while neurons in the upper layers exhibit no immunolabeling and have less synaptic structures in experimental animals than in control. We suggest that neurons in the hippocampal structures are involved in the regulation of functions and formation of prenatal pathology.
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Gaudet, C., Lim, Y.-P., Stonestreet, B., and Threlkeld, S., Effects of age, experience and interalpha inhibitor proteins on working memory and neuronal plasticity after neonatal hypoxia–ischemia, Behav. Brain Res., 2016, vol. 302, pp. 88–99.
Hayward, C. and Sanborn, K., Puberty and the emergence of gender diferences in psychopathology, J. Adolescent Health, 2002, vol. 30, pp. 49–58.
Vinogradova, O.S., Gippokamp i pamyat’ (Hippocampus and Memory), Moscow, 1975.
Buchanan, C., Eccles, J., and Becker, J., Are adolescents the victims of raging hormones: evidence for activational effects of hormones on moods and behavior at adolescence, Psychol. Bull., 1992, vol. 111, pp. 62–107.
Huerta, R. and Brizuela-Gamino, O.L., Interaction of pubertal status, mood and self-esteem in adolescent girls, J. Reprod. Med., 2002, vol. 47, pp. 217–225.
Modesti, P., Pela, I., Cecioni, I., Gensini, G., Serneri, G., and Bartolozzi, G., Changes in blood pressure reactivity and 24-hour blood pressure profile occurring at puberty, Angiology, 2006, vol. 45, pp. 443–450.
Paxinos, G. and Watson, C., The Rat Brain in Stereotaxic Coordinates, 5nd Ed., Academic Press, 2004.
Guthmann, A., Fritschy, J., Ottersen, O., Torp, R., and Herbert, H., GABA, GABA transporters, GABA (A) receptor subunits and GAD mRNAs in the rat parabrachial and Kölliker–Fuse nuclei, J. Comp. Neurol., 1998, vol. 400, no. 2, pp. 229–243.
Olenev, S.N. and Olenev, A.S., Neirobiologiya-95 (Neurobiology-95), St. Petersburg, 1995.
Mtchedlishvili, Z., Lepsveridze, E., Xu, H., Kharlamov, E., Lu, B., and Kelly, K., Increase of GABAA receptor-mediated tonic inhibition in dentate granule cells after traumatic brain injury, Neurobiol. Disease, 2010, vol. 38, pp. 464–475.
Pirker, S., Schwarzer, C., Wieselthaler, A., Sieghart, W., and Sperk, G., GABAA receptors: immunocytochemical distribution of 13 subunits in the adult rat brain, Neurosci., 2000, vol. 101, pp. 815–850.
Sperk, G., Schwarzer, C., Tsunashima, K., Fuchs, K., and Sieghart, W., GABAA receptor subunits in the rat hippocampus I: immunocytochemical distribution of 13 subunits, Neurosci., 1997, vol. 80, pp. 987–1000.
Sur, C., Farrar, S., Kerby, J., Whiting, P., Atack, J., and McKernan, R., Preferential coassembly of a4 and d subunits of the GABAA receptor in rat thalamus, Mol. Pharmacol., 1999, vol. 56, pp. 110–115.
Wisden, W., Laurie, D., Monyer, H., and Seeburg, P., The distribution of 13 GABAA receptor subunit mRNAs in the rat brain. I. Telencephalon, diencephalon, mesencephalon, Neurosci., 1992, vol. 12, pp. 1040–1062.
Shen, H., Sabaliauskas, N., Sherpa, A., Fenton, A., Stelzer, A., Aoki, C., and Smith, S., A critical role for a4βd GABAA receptors in shaping learning deficits at puberty in mice, Science, 2010, vol. 327, pp. 1515–1518.
Sabaliauskas, N., Shen, H., Homanics, G., Smith, S., and Aoki, C., Knockout of the δ-aminobutyric acid receptor subunit a4 reduces functional d-containing extrasynaptic receptors in hippocampal pyramidal cells at the onset of puberty, Brain Res., 2012, vol. 1450, pp. 11–23.
Smith, S., Aoki, C., and Shen, H., Puberty, steroids and GABAA receptor plasticity, Psychoneuroendocrinol., 2009, vol. 34, no. 1, pp. 91–103.
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Original Russian Text © L.I. Khozhai, V.A. Otellin, 2017, published in Zhurnal Evolyutsionnoi Biokhimii i Fiziologii, 2017, Vol. 53, No. 6, pp. 448—452.
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Khozhai, L.I., Otellin, V.A. Distribution of GAD67-expressing neurons and morphological changes in hippocampal structures during pubertal period after acute perinatal hypoxia in rats. J Evol Biochem Phys 53, 505–510 (2017). https://doi.org/10.1134/S0022093017060084
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DOI: https://doi.org/10.1134/S0022093017060084