Activity of Hydroxy Derivatives of Chalcones toward Superoxide Anion Radical

Abstract

Trapping activity of new hydroxy derivatives of chalcones toward electrochemically generated superoxide anion radical (${\text{O}}_{2}^{{\bullet \, - }}$) in a xanthine/xanthine oxidase enzymatic system upon reduction of nitro blue tetrazolium and during non-enzymatic quinoid oxidation of adrenaline in alkaline medium has been studied. Antiradical activity of new hydroxy derivatives of chalcones toward ${\text{O}}_{2}^{{\bullet \, - }}$ has been established for the first time. Reaction with electrochemically generated ${\text{O}}_{2}^{{\bullet \, - }}$ and higher antiradical activity under adrenaline autooxidation conditions in alkaline medium has been revealed for chalcone containing 2,6-di-tert-butylphenol fragment. Chalcone with two non-shielded OH groups showed the highest trapping activity in xanthine/xanthine oxidase enzymatic system and SOD-protective activity. The inhibiting activity of hydroxy derivatives of chalcones toward ${\text{O}}_{2}^{{\bullet \, - }}$ agrees well with in silico prediction and indicates their ability to decrease probability of oxidative stress development. Correlation has been found between the ability of the studied chalcones to consume superoxide anion radical and inhibiting activity in model systems of peroxide oxidation of oleic acid and tilapia liver lipids.