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A Theory of Charge Selectivity Reversal in Cation- or Anion-Selective Tight Junctions between Epithelial Cells: A Nonlocal Electrostatic Approach

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Abstract

A theory was developed to explain how charge selectivity is reversed in tight junctions (TJs) between epithelial cells when the sign of the TJ fixed charge changes. An original method that allows for the ion cavity effect was used to calculate the changes in ion solvation energies that occur upon ion transfer in TJs. An equation was derived to describe the Na+ to Cl– permeability ratio of TJs as a function of the concentration and sign of TJ fixed charges and the correlation length of water. The theory agrees with published experimental data that Na+/Cl– selectivity is reversed in Madin–Darby canine kidney (MDCK) cells when claudin-15 protein molecules with a changed sign of amino acid residues are expressed in TJs.

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Funding

This work was supported by state contract no. AAAA-A17-117032350032-3 (A.A. Rubashkin) and the Ministry of Science and Education of the Russian Federation (state contract no. AAAA-A20-120101090002-4, M.A. Vorotyn-tsev).

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Authors and Affiliations

  1. Institute of Cytology, Russian Academy of Sciences, 194064, St. Petersburg, Russia

    A. A. Rubashkin

  2. Downstate Medical Center, State University of New York, 11203, Brooklyn, NY, United States

    P. Iserovich

  3. Frumkin Institute of Physical Chemistry and Electrochemistry, Russian Academy of Sciences, 119071, Moscow, Russia

    M. A. Vorotyntsev

  4. Institute for Problems of Chemical Physics, Russian Academy of Sciences, 142432, Chernogolovka, Moscow oblast, Russia

    M. A. Vorotyntsev

Authors
  1. A. A. Rubashkin
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  2. P. Iserovich
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  3. M. A. Vorotyntsev
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Corresponding author

Correspondence to A. A. Rubashkin.

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Conflict of interests. The authors declare that they have no conflict of interest.

This work does not contain any studies involving animals or human subjects performed by any of the authors.

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Translated by T. Tkacheva

Abbreviations: TJ, tight junction; MDCK, Madin–Darby canine kidney (cells).

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Rubashkin, A.A., Iserovich, P. & Vorotyntsev, M.A. A Theory of Charge Selectivity Reversal in Cation- or Anion-Selective Tight Junctions between Epithelial Cells: A Nonlocal Electrostatic Approach. BIOPHYSICS 66, 84–90 (2021). https://doi.org/10.1134/S0006350921010127

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  • DOI: https://doi.org/10.1134/S0006350921010127

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