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The Effects of a Low-Frequency Electric Field on Recombinant Luciferase Activity

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Abstract

Studies on the effects of an electromagnetic field on the activity of recombinant luciferase in the luciferase–luciferin–ATP-Mg2+ system were conducted. The enzymatic activity was evaluated by the measurements of biochemiluminescence intensity with a standard chemiluminometer. Frequencies that effectively caused irreversible changes in the enzyme activity were identified. The intensity of chemiluminescence (Icl), after it reached the stationary level, and the time constant of the intensity decay, after cessation of the stationary luminescence due to ATP depletion, were the criteria of the luciferase activity. I0 sharply increased from (250 ± 47) imp/s to (1250 ± 75) imp/s compared to the control under exposure to an electromagnetic field of 6 Hz. At the same time, the duration of the stationary luminescence decreased by approximately five times (from 30 to 5.5 min). The decay of luminescence, τ, accelerated significantly (by nine times relative to the control). An opposite effect of other experimentally chosen frequencies of the electromagnetic field was obtained. As an example, upon exposure to the electromagnetic field with frequencies of 12, 48, and 96 Hz, the luciferase activity decreased by 2 times (at 48 and 96 Hz) and by 4 times (at 12 Hz) compared to the control. Luciferase activity did not differ from the control after exposure to a field of 24 Hz.

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REFERENCES

  1. P. V. Dunlap and K. Kita-Tsukamoto, in The Prokaryotes, Vol. 2: Ecophysiology and Biochemistry, Ed. by M. Dworkin (Springer, New York, 2006), pp. 863–892.

  2. N. S. Rodionova, Extended Abstract of Candidate’s Dissertation in Biology (Krasnoyarsk, 2004).

  3. A. V. Sosnov, Razrabotka Registtr. Lekarstv. Sredstv, No. 4, 108 (2017).

    Google Scholar 

  4. I. V. Berezin, L. Yu. Brovko, and N. I. Ugarova, Bioorg. Khim. 3 (12), 1589 (1977).

    Google Scholar 

  5. V. K. Uteshev, T. N. Pashovkin, and E. N. Gakhova, Vestn. Nov. Med. Tekhnol., No. 4, 7 (2010).

  6. A. A. Oleshkevich, Biophysics 62 (4), 603 (2017).

    Article  Google Scholar 

  7. D. J. Panagopoulos, A. Karabarbounis, and L. H. Margaritisa, Biochem. Biophys. Res. Commun. 298, 95 (2002).

    Article  Google Scholar 

  8. M. S. Pashovkina, I. G. Akoev, and T. N. Pashovkin, in Biological Effects of Weak Electromagnetic Radiation (Pushchino, 2002), pp. 26–37 [in Russian].

    Google Scholar 

  9. M. S. Pashovkina and I. G. Akoev, Biophysics (Moscow) 45 (1), 121 (2000).

    Google Scholar 

  10. T. N. Pashovkin, in Abstr. Book of the 5th Int. Symp. on Therap. Ultrasound (Harvard Medical School, Boston, 2005), p. 77.

  11. A. A. Oleshkevich, A. M. Nosovskii, and E. V. Kaminskaya, Biomed. Radioelektron., No. 2, 53 (2014).

  12. A. A. Oleshkevich, Veterinarnaya Meditsina, No. 3–4, 35 (2012).

  13. N. N. Ugarova, M. I. Koksharov, and G. Yu. Lomakina, RF Patent No. RU 2 420 594 (2009).

  14. A. D. Usanov et al., Biomed. Radioelektron., No. 8, 32 (2015).

  15. A. D. Usanov et al., Khim.-Farm. Zh. 39 (8), 65 (2005).

    Google Scholar 

  16. V. V. Lednev, Biofizika 41 (1), 224 (1996).

    Google Scholar 

  17. M. A. Derkho and T. I. Sereda, Izv. Orenburg. Gos. Agr. Univ., No. 1 (57), 72 (2016).

  18. I. M. Golev et al., Biomed. Radioelektron., No. 8, 25 (2015).

  19. M. S. Pashovkina and T. N. Pashovkin, Radiats. Biol. Radioekol. 51 (3), 1 (2011).

    Google Scholar 

  20. L. D. Johns, J. Athletic Training 37 (3), 293 (2002).

    Google Scholar 

  21. V. E. Novikov, A. A. Oleshkevich, and M. A. Danilova, in Scientific Papers of the 6th Russian Congress of Biophysicists (Plekhanovets, Krasnodar, 2019), Vol. 2, p. 48 [in Russian].

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Authors and Affiliations

  1. Skryabin Moscow State Academy of Veterinary Medicine and Biotechnology, 109472, Moscow, Russia

    A. A. Oleshkevich, V. E. Novikov & M. A. Danilova

Authors
  1. A. A. Oleshkevich
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  2. V. E. Novikov
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  3. M. A. Danilova
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Correspondence to A. A. Oleshkevich.

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Translated by E. Puchkov

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Oleshkevich, A.A., Novikov, V.E. & Danilova, M.A. The Effects of a Low-Frequency Electric Field on Recombinant Luciferase Activity. BIOPHYSICS 65, 557–563 (2020). https://doi.org/10.1134/S0006350920040132

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  • DOI: https://doi.org/10.1134/S0006350920040132

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