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Oxidative Modification of Proteins in the Tissues of Rats with Growing Tumors under the Ozone-Photodynamic Treatment

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Abstract—Ozone-photodynamic effects on tumor growth rate and oxidative modification of proteins in rat tissues were studied using a model of PA kidney carcinoma. It was shown that the developed experimental therapeutic method based on the joint parenteral intraperitoneal administration of an ozonated saline solution with a concentration of ozone in the ozone–oxygen mixture of 400 μg/L and a 0.3% solution of the Photosens photosensitizer intratumorally with the local action of light with a wavelength of 660 ± 10 nm for 10 min reduced the intensity of tumor growth. Normalization of the prooxidant/antioxidant balance in experimental animals was observed upon combined use of ozonated saline solution and photodynamic therapy.

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REFERENCES

  1. V. V. Kuznetsov, Issled. Prakt. Med. 2 (4), 98 (2015).

    Article  Google Scholar 

  2. A. B. Gapeyev, T. G. Shcherbatyuk, Biol. Membrany 37 (3), 163 (2020).

  3. T. G. Shcherbatyuk, Nizhegorod. Med. Zh. 1, 52 (2003).

    Google Scholar 

  4. T. G. Shcherbatyuk, Fiziol. Zh. 54, 41 (2008).

    Article  Google Scholar 

  5. T. G. Shcherbatyuk, V. D. Selemir, and E. S. Klintsova, RF Patent No. 2361590 (2007).

  6. E. S. Knyazeva, T. G. Shcherbatyuk, and A. B. Gapeev, Vestn. Fizioter. Kurortol. 25, 37 (2019).

    Google Scholar 

  7. Ya. Buresh, O. Bureshova, and D. P. Houston, Methods and Basic Experiments in Studies on the Brain and Behavior (Vysshaya Shkola, Moscow, 1991) [in Russian].

    Google Scholar 

  8. N. V. Vdovina, E. S. Klintsova, and T. G. Shcherbatyuk, Sovrem. Tekhnol. Med. 2, 12 (2010).

    Google Scholar 

  9. T. G. Shcherbatyuk, I. A. Chernigina, E. S. Plekhanova, and A. B. Gapeev, Aktual. Vopr. Biol. Fiz. Khim. 4, 564 (2019).

    Google Scholar 

  10. S. N. Buranov, V. V. Gorokhov, V. I. Karelin, and V. D. Selemir, RF Patent No. RUS 2249445 (2003).

  11. E. V. Yaroslavtseva-Isaeva, M. A. Kaplan, Yu. S. Romanko, et al., Ross. Bioterapevt. Zh. 2, 19 (2003).

    Google Scholar 

  12. K. E. Koptyaeva, A. A. Muzhikyan, Ya. A. Gushchin, et al., Lab. Zhivotn. Nauch. Issled. 2, 71 (2018).

    Google Scholar 

  13. Yu. I. Gubskii, I. F. Belenichev, E. L. Levitskii, et al., Sovrem. Probl. Toksikol. 8, 20 (2005).

    Google Scholar 

  14. A. V. Arutyunyan, E. E. Dubinina, and N. N. Zybina, Methods for Assessing Free Radical Oxidation and the Antioxidant System of the Body (IKF Foliant, St. Petersburg, 2000) [in Russian].

    Google Scholar 

  15. E. E. Dubinina, S. O. Burmistrov, D. A. Khodov, and I. S. Porotov, Vopr. Med. Khim. 41, 24 (1995).

    Google Scholar 

  16. E. I. Kuz’mina, A. S. Nelyubin, and M. K. Shchennikova, in Biochemistry and Biophysics of Microorganiosms (Gorky State Univ., Gorky, 1983), pp. 41–48 [in Russian].

    Google Scholar 

  17. E. V. Markova, Behavior and Immunity (NGPU, Novosibirsk, 2013) [in Russian].

    Google Scholar 

  18. B. L. Zharkoi, in Free Radicals, Antioxidants, and Animal Health (Voronezh State Univ.,Voronezh, 2004), pp. 36–40 [in Russian].

    Google Scholar 

  19. Yu. V. Nikitina, Extended Abstract of Candidate’s Dissertation in Biology (Nizhny Novgorod, 2009).

  20. W. Girotti, J. Lipid Res. 39, 1529 (1998).

    Google Scholar 

  21. Yu. P. Kozlov, Free Radicals and Their Role in Normal and Patholgical Processes (Moscow State Univ., Moscow, 1973) [in Russian].

    Google Scholar 

  22. L. B. Dudnik, L. M. Viksna, A. Ya. Maiore, Vopr. Med. Khim. 46, 597 (2000).

    Google Scholar 

  23. M. M. Sozarukova, E. V. Proskurina, Yu. A. Vladimirov, Vestn. RGMU 1, 61 (2016).

    Google Scholar 

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Funding

The research was carried out with the financial support of the Russian Foundation for Basic Research, project no. 19-02-00667.

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Authors and Affiliations

  1. Privolzhsky Research Medical University, Ministry of Health of the Russian Federation, 603005, Nizhny Novgorod, Russia

    T. G. Shcherbatyuk, E. S. Zhukova (Plekhanova) & Ju. V. Nikitina

  2. Pushchino State Institute of Natural Sciences, 142290, Pushchino, Moscow oblast, Russia

    T. G. Shcherbatyuk

  3. Institute of Cell Biophysics, Russian Academy of Sciences, 142290, Pushchino, Moscow oblast, Russia

    A. B. Gapeyev

Authors
  1. T. G. Shcherbatyuk
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  2. E. S. Zhukova (Plekhanova)
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  3. Ju. V. Nikitina
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  4. A. B. Gapeyev
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Corresponding author

Correspondence to T. G. Shcherbatyuk.

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CONFLICT OF INTEREST

The authors declare no conflict of interest.

COMPLIANCE WITH ETHICAL STANDARDS

All manipulations with animals were carried out in accordance with the recommendations established by the European Convention for the protection of vertebrates used for experimental and other scientific purposes (Strasbourg, 2006), and in accordance with the Helsinki Declaration on the humane treatment of animals (Edinburgh, 2000). The work was approved by the Local Ethics Committee of PRMU.

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Translated by E. Puchkov

Abbreviations: PDT, photodynamic therapy; OPS, ozonated physiological saline; OMP, oxidative modification of proteins; DNPH, dinitrophenylhydrazones; TBA, thiobarbituric acid; EB, exploratory behavior.

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Shcherbatyuk, T.G., Zhukova (Plekhanova), E.S., Nikitina, J.V. et al. Oxidative Modification of Proteins in the Tissues of Rats with Growing Tumors under the Ozone-Photodynamic Treatment. BIOPHYSICS 65, 319–326 (2020). https://doi.org/10.1134/S0006350920020219

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  • DOI: https://doi.org/10.1134/S0006350920020219

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