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Correction of Cold Damage to Mammalian Erythrocytes by Chlorpromazine to Influence the Dynamic Structure of a Membrane

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Abstract—The resistance of mammalian erythrocytes (human, dog, horse, bull, and rabbit) to cold shock in the presence of chlorpromazine was studied; its effect on the state of erythrocyte membranes was investigated. The bond strength between membrane phospholipids of mammalian erythrocytes and the characteristics of the cold shock of these cells was determined with the correlation analysis. It has been established that erythrocytes of mammals whose membranes contain a high content of sphingomyelin and phosphatidylethanolamine and a low content of phosphatidylcholines are more resistant to cold shock. Chlorpromazine showed high efficiency in protecting mammalian erythrocytes against cold shock. The dynamic structure of membranes of human erythrocytes treated with the chlorpromazine was studied by EPR of spin-probes. The use of a set of spin-labeled probes: palmitic acid amide, 5-DOXYL stearic acid, and 16-DOXYL stearic acid, which allow studying the micro-viscosity parameters of the lipid bilayer of erythrocyte membranes in the transmembrane direction, made it possible to establish the locations of chlorpromazine action, namely, in the region of polar heads and hydrophobic tails of membrane phospholipids.

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Authors and Affiliations

  1. Institute for Problems of Cryobiology and Cryomedicine, National Academy of Sciences of Ukraine, 61016, Kharkiv, Ukraine

    N. M. Shpakova, N. V. Orlova & S. S. Yershov

Authors
  1. N. M. Shpakova
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  2. N. V. Orlova
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  3. S. S. Yershov
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Correspondence to N. M. Shpakova.

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Conflict of interest. The authors declare that they have no conflict of interest.

Statement of compliance with standards of research involving humans as subjects. All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki Declaration and its later amendments or comparable ethical standards. Informed consent was obtained from all individual participants involved in the study.

Statement on the welfare of animals. Animal blood preparation and all manipulations were carried out in accordance with domestic and international bioethical norms, materials of the IV European Convention for the Protection of Vertebrate Animals used for Experimental and other Scientific Purposes (ETS 123) (Strasbourg, 1986).

Additional information

Translated by I. Shipounova

Abbreviations: CPZ, chlorpromazine; PAA, palmitic acid amide; 5-DS, 5-DOXYL stearic acid; 16-DS, 16-DOXYL stearic acid.

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Shpakova, N.M., Orlova, N.V. & Yershov, S.S. Correction of Cold Damage to Mammalian Erythrocytes by Chlorpromazine to Influence the Dynamic Structure of a Membrane. BIOPHYSICS 64, 367–373 (2019). https://doi.org/10.1134/S0006350919030205

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  • DOI: https://doi.org/10.1134/S0006350919030205

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