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Estimation of biophysical characteristics for Chlamydomonas reinhardtii pigment mutants with an M-PEA-2 fluorometer

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Abstract

Light green pigment mutants with a reduced chlorophyll b content were constructed in the microalga Chlamydomonas reinhardtii Dangeard. A simultaneous recording of the induction curves for prompt and delayed fluorescence and the redox state of P700 in the microsecond range with a M-PEA-2 fluorometer revealed decreases in the quantum yield of electron transport in PS2 (φE 0) and the performance index (PI ABS) and increases in the quantum efficiency of energy dissipation (φD 0) and ΔpH-dependent nonphotochemical quenching (qE and NPQ). The light-dependence curves of the fluorescence parameters confirmed a decrease in the coefficient of maximum utilization of light energy (α) for the mutants. However, the mutants showed an adequate rate of electron transport at a medium light intensity under steady-state conditions. The mutations did not directly affect the oxidation reactions of the PS1 pigment (P700) and the decrease in delayed fluorescence. Experience in using the mutants to test polluted waters of Kazakhstan confirmed that the mutants are promising for use in biomonitoring for mutagens.

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Abbreviations

PS1:

photosystem I

PS2:

photosystem 2

DF:

delayed fluorescence

ETR:

relative noncyclic electron transport rate

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Authors and Affiliations

  1. Department of Biology, Moscow State University, Moscow, 119991, Russia

    D. N. Matorin, F. F. Protopopov & L. B. Bratkovskaja

  2. Institute of Physics and Technology, Ammosov North-Eastern Federal University, ul. Kulakovskogo 48, Yakutsk, 677000, Russia

    F. F. Protopopov & A. A. Alekseev

  3. Al-Farabi Kazakh National University, pr. Al-Farabi 71, Almaty, 050040, Republic of Kazakhstan

    A. K. Sadvakasova & B. K. Zayadan

Authors
  1. D. N. Matorin
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  2. F. F. Protopopov
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  3. A. K. Sadvakasova
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  4. A. A. Alekseev
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  5. L. B. Bratkovskaja
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  6. B. K. Zayadan
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Corresponding authors

Correspondence to D. N. Matorin, F. F. Protopopov or A. K. Sadvakasova.

Additional information

Original Russian Text © D.N. Matorin, F.F. Protopopov, A.K. Sadvakasova, A.A. Alekseev, L.B. Bratkovskaja, B.K. Zayadan, 2016, published in Biofizika, 2016, Vol. 61, No. 4, pp. 717–725.

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Matorin, D.N., Protopopov, F.F., Sadvakasova, A.K. et al. Estimation of biophysical characteristics for Chlamydomonas reinhardtii pigment mutants with an M-PEA-2 fluorometer. BIOPHYSICS 61, 606–613 (2016). https://doi.org/10.1134/S0006350916040151

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  • DOI: https://doi.org/10.1134/S0006350916040151

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