Abstract
In order to determine the cytotoxic or cytoprotective effect of the synthetic isoflavonoid genistein, we studied its effect on HeLa tumor cells, which contain estrogen alpha receptors and do not contain estrogen beta receptors. It was shown that the genistein concentration (IC 50 = 0.2 mM) at which the half maximal inhibition of the HeLa cell viability is achieved is ten times higher than the concentrations of tamoxifen and cisplatin, which are reference agents with a cytotoxic effect. At micromolar concentrations (0.1–10 µM) genistein decreased the cytotoxic effects of cisplatin and tamoxifen. We found the reduced Bax mRNA expression and increased Bcl-2 mRNA expression during incubation of the cells with genistein, which also indicates its cytoprotective anti-apoptotic effect. Genistein, even in high concentrations, had no effect on the membrane potential and calcium capacity of isolated mitochondria and did not activate the opening of the Ca2+-induced mitochondrial pore. Thus, the data show a protective effect of the isoflavonoid genistein on tumor cells.
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Abbreviations
- ERalpha:
-
alpha estrogen receptors
- ERbeta:
-
beta estrogen receptors
References
D. G. Pons, M. Nadal-Serrano, M. Torrens-Mas, et al., J. Cell Biochem. 22 (2015). doi 10.1002/jcb.25268
P. Xiong, R. Wang, X. Zhang, et al., Anticancer Agents Med. Chem. 20 (2015).
G. Ouyang, L. Yao, K. Ruan, et al., Cell Biol. Int. 33, 1237 (2009).
A. Hussain, G. Harish, and S. A. Prabhu, Cancer Epidemiol. 36, e387 (2012).
A. V. Semeikin, T. A. Fedotcheva, I. S. Levina, et al., Khim.-Farm. Zh. 48 (6) 9 (2014).
E. E. Nifantyev, A. M. Koroteev, and A. M. Pozdeev, Khim.-Farm. Zh. 49 (2), 8 (2015).
H. Q. Li, Y. Luo, and C. H. Qiao, Mini Rev. Med. Chem. 12, 350 (2012).
S. Aufderklamm, F. Miller, A. Galasso, et al., Cancer Res. 202, 101 (2014). doi: 10.1007/978-3-642-451959_12.
D. K. Gong, B. H. Liu, and X. Tan, Human Drug Res. (Stuttgart) 65 (2) 65 (2015). doi 10.1055/s-00341372595
K. Sahin, M. Tuzcu, N. Basak, et al., J. Oncol. 2012: 461562 (2012). doi 10.1155/2012/461562
E. K. Kim, K. B. Kwon, M. Y. Song, et al., Mol. Cell. Endocrinol. 278 (1–2) 18 (2007).
T. A. Fedotcheva, N. I. Fedotcheva, V. V. Teplova, et al., Vopr. Biol. Med. Farm. Khim. 11 (11), 158 (2013).
T. A. Fedotcheva, A. G. Akopdjanov, N. L. Shimanovskii, et al., Biophysics (Moscow) 59 (5), 732 (2014).
T. A. Fedotcheva, A. G. Kruglov, V. V. Teplova, et al., Biophysics (Moscow) 57 (6) 792 (2012).
J. C. Ashton, Cancer Res. 75 (11), 2400 (2015). doi 10.1158/0008-5472.CAN-14-3763
K. Morito, T. Hirose, J. Kinjo, et al., Biol. Pharm. Bull. 24, 351 (2001).
H. Zhang, G. Liu, X. Zeng, et al., Int. J. Nanomed. 10, 2461 (2015). doi: 10.2147/IJN.S78988.
L. Varinska, P. Gal, G. Mojzisova, and L. Mirossay, Int. J. Mol. Sci. 16 (5) 11728 (2015). doi 10.3390/ijms160511728
T. A. Fedotcheva, N. L. Shimanovskii, A. I. Senderovich, et al., Khim.-Farm. Zh. 41 (7), 3 (2007).
M. W. Lee, J. H. Bach, H. J. Lee, et al., Cancer Invest. 23 (7) 586 (2005).
S. H. Kim, S. H. Kim, S. C. Lee, and Y. S. Song, Ann. N.Y. Acad. Sci. 1171, 196 (2009). doi 10.1111/j.17496632.2009.04902.x
P. Krejbich-Trotot, M. Denizot, J. J. Hoarau, et al., FASEB J. 25 (1) 314 (2011). doi 10.1096/fj.10-164178
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Original Russian Text © T.A. Fedotcheva, K.E. Shirokikh, A.I. Matyushin, V.M. Rzheznikov, V.Yu. Kovtun, N.L. Shimanovskii, 2015, published in Biofizika, 2015, Vol. 60, No. 6, pp. 1151–1156.
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Fedotcheva, T.A., Shirokikh, K.E., Matyushin, A.I. et al. The cytoprotective effect of the phytoestrogen genistein on tumor cells. BIOPHYSICS 60, 957–962 (2015). https://doi.org/10.1134/S000635091506007X
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DOI: https://doi.org/10.1134/S000635091506007X