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The physicochemical properties of histone H2A and modified histone H2A-TAT complexes with plasmid DNA

  • Molecular Biophysics
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Abstract

Histone H2A is capable of delivering transgenic DNA into mammalian cells in vitro. Its ability to deliver DNA in vivo is unknown as yet, but the factors that affect the efficiency of in vivo delivery can be estimated during in vitro experiments. The first step is estimating the size and ζ potential of H2A–DNA complexes. Recombinant histone H2A was obtained along with its modification containing a TAT peptide, which originates from the human immunodeficiency virus TAT protein and is used to improve the efficiency of delivering macromolecules into mammalian cells. The effective diameter and ζ potential were measured for particles that form in a mixture of histone H2A with DNA and the effect of the TAT peptide on the parameters was studied. Positive ζ potentials were observed for DNA complexes with histone H2A or modified histone H2A-TAT. The effective diameter of H2A–DNA complexes was approximately 200 nm, while histone modification with the TAT peptide caused aggregation of complexes to produce large particles of approximately 1 µm in diameter.

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Authors and Affiliations

  1. Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, ul. Miklukho-Maklaya 16/10, Moscow, 117997, Russia

    A. V. Vvedenskii, S. V. Sizova & A. I. Kuzmich

  2. Biology Department, Moscow State University, Moscow, 119991, Russia

    A. V. Vvedenskii

  3. Institute of Molecular Genetics, Russian Academy of Sciences, pl. Akademika Kurchatova 2, Moscow, 123182, Russia

    A. I. Kuzmich

Authors
  1. A. V. Vvedenskii
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  2. S. V. Sizova
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  3. A. I. Kuzmich
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Correspondence to A. V. Vvedenskii.

Additional information

Original Russian Text © A.V. Vvedenskii, S.V. Sizova, A.I. Kuzmich, 2015, published in Biofizika, 2015, Vol. 60, No. 5, pp. 883–888.

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Vvedenskii, A.V., Sizova, S.V. & Kuzmich, A.I. The physicochemical properties of histone H2A and modified histone H2A-TAT complexes with plasmid DNA. BIOPHYSICS 60, 727–731 (2015). https://doi.org/10.1134/S0006350915050231

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  • DOI: https://doi.org/10.1134/S0006350915050231

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