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Jasmonic Acid Induces Endoplasmic Reticulum Stress with Different Outcome in Cultured Normal and Tumor Epidermal Cells

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Abstract

Plant hormones produce cytotoxic effect on human cells and can trigger the processes unrelated to cell death, e.g., biosynthetic system stress. The goal of this study was to investigate activation of the endoplasmic reticulum (ER) stress by jasmonic acid (JA) and to distinguish between the responses of cultured immortalized non-tumorigenic HaCaT cells and epidermoid carcinoma A431 cells to this plant hormone. JA was used in the concentration of 2 mM, as it suppressed cell proliferation in both cell lines. We analyzed expression of genes associated with the activation of ER stress (GRP78, ATF4, CHOP), the structure of the ER and Golgi complex, and synthetic processes in the HaCaT and A431 cell lines. JA induced expression of genes responsible for the activation of ER stress and caused hypertrophic changes in the Golgi complex in both cell lines. However, the patterns of gene expression in the HaCaT and A431 cells were different, and higher levels of involucrin synthesis were observed in A431 but not in HaCaT cells, suggesting that JA activated differentiation of the tumor A431 cells only. Therefore, JA induced ER stress in both cell lines, but the consequences of ER stress were different for the epidermal immortalized non-tumorigenic and tumor cells.

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Abbreviations

DAPI:

4,6-diamidino-2-phenylindole hydrochlorid

DMSO:

dimethyl sulfoxide

DTT:

dithiothreitol

ER:

endoplasmic reticulum

JA:

jasmonic acid

qPCR:

real-time polymerase chain reaction

PI:

propidium iodide

TEM:

transmission electron microscopy

TGN:

trans-Golgi network

UPR:

unfolded protein response

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Funding

Funding. The work was supported by the Russian Foundation for Basic Research (project 19-015-00233) and the Development Program of the Moscow State University (complex FACSAria SORP).

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Authors and Affiliations

  1. Lomonosov Moscow State University, Faculty of Biology, 119991, Moscow, Russia

    M. S. Vildanova, A. A. Saidova, D. M. Potashnikova, G. E. Onishchenko & E. A. Smirnova

  2. Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, 119992, Moscow, Russia

    A. I. Fokin

Authors
  1. M. S. Vildanova
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  2. A. A. Saidova
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  3. A. I. Fokin
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  4. D. M. Potashnikova
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  5. G. E. Onishchenko
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  6. E. A. Smirnova
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Corresponding author

Correspondence to M. S. Vildanova.

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Compliance with ethical standards. This article does not contain any studies involving animals or human participants performed by any of the authors.

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Conflict of interest. The authors declare no conflict of interest.

Russian Text © The Author(s), 2019, published in Biokhimiya, 2019, Vol. 84, No. 9, pp. 1289–1300.

Originally published in Biochemistry (Moscow) On-Line Papers in Press, as Manuscript BM19-088, August 5, 2019.

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Vildanova, M.S., Saidova, A.A., Fokin, A.I. et al. Jasmonic Acid Induces Endoplasmic Reticulum Stress with Different Outcome in Cultured Normal and Tumor Epidermal Cells. Biochemistry Moscow 84, 1047–1056 (2019). https://doi.org/10.1134/S0006297919090074

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