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Metagenomics of bolidophyceae in plankton and ice of the White Sea

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Abstract

The molecular diversity of poorly studied algae of Bolidophyceae class was first estimated by Illumina sequencing of V4 region of 18S rRNA gene in ice, under-ice water and summer water of the subarctic White Sea. We used two clustering thresholds–93 and 97%–and revealed 31 phylotypes of Bolidophyceae. Triparma pacifica and Т. strigata were identified to species level. The association of individual phylotypes to certain biotopes (ice or plankton) and stages of seasonal succession (under ice or summer plankton) has been established. Some phylotypes are found in different biotopes and over a wide temperature range. Due to changing their genetic composition, Bolidophyceae are a constant component of the photoautotrophic plankton and ice communities.

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Abbreviations

BA:

Bayesian analysis

ML:

maximum likelihood (method)

OTU:

operational taxonomic unit

st.:

station

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Authors and Affiliations

  1. Lomonosov Moscow State University, Faculty of Biology, 119991, Moscow, Russia

    T. A. Belevich & L. V. Ilyash

  2. Lomonosov Moscow State University, Belozersky Institute of Physico-Chemical Biology, 119991, Moscow, Russia

    I. A. Milyutina, M. D. Logacheva & A. V. Troitsky

  3. Kazan Federal University, Institute of Fundamental Biology and Medicine, Laboratory of Extreme Biology, 420012, Kazan, Russia

    M. D. Logacheva

Authors
  1. T. A. Belevich
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  2. L. V. Ilyash
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  3. I. A. Milyutina
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  4. M. D. Logacheva
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  5. A. V. Troitsky
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Correspondence to A. V. Troitsky.

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Original Russian Text © T. A. Belevich, L. V. Ilyash, I. A. Milyutina, M. D. Logacheva, A. V. Troitsky, 2017, published in Biokhimiya, 2017, Vol. 82, No. 12, pp. 1917-1928.

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Belevich, T.A., Ilyash, L.V., Milyutina, I.A. et al. Metagenomics of bolidophyceae in plankton and ice of the White Sea. Biochemistry Moscow 82, 1538–1548 (2017). https://doi.org/10.1134/S0006297917120136

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  • DOI: https://doi.org/10.1134/S0006297917120136

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