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Analysis of four circulating complexes of insulin-like growth factor binding proteins in human blood during aging

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Abstract

The primary role of insulin-like growth factor binding proteins (IGFBPs) is to regulate availability of IGFs for interacting with receptors, but IGFBPs perform IGF-independent actions as well. The availability and activity of IGFBPs in the circulation is influenced primarily by their concentration and structural modifications, but possibly also by interaction with major plasma proteins such as transferrin, alpha-2-macroglobulin (α2M), and fibrinogen. Four types of circulating IGFBP complexes were examined in this study by immuno- and ligand-binding assays in adults of different age. The amounts of IGFBP-3/transferrin and IGFBP-1/fibrinogen complexes were similar in middle- and old-aged persons, whereas the amounts of IGFBP-1 (or -2)/α2M monomer complexes were lower in the old-aged group and negatively correlated with total IGFBP-1 (or -2) amounts in blood. In contrast to IGFBP-1, IGFBP-2 was present in significantly greater quantities in complexes with α2M dimer than α2M monomer in older individuals. IGFBP complexes did not bind 125I-labeled IGF-I in amounts detectable by ligand blotting. According to the results of this study, the quantities of IGFBP-1 and IGFBP-2, which interact with α2M, are age-dependent and, in the case of complexes with α2M monomer, they are negatively correlated with the total circulating levels of these two IGFBPs.

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Abbreviations

α2M:

alpha-2-macroglobulin

BMI:

body mass index

DU:

densitometric unit

IGF:

insulin-like growth factor

IGFBP:

IGF-binding protein

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Authors and Affiliations

  1. INEP-Institute for the Application of Nuclear Energy, University of Belgrade, Belgrade, Serbia

    O. Nedić, M. Šunderić, N. Gligorijević & G. Miljuš

  2. Clinical-Medical Centre “Bežanijska Kosa”, Belgrade, Serbia

    V. Malenković

Authors
  1. O. Nedić
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  2. M. Šunderić
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  3. N. Gligorijević
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  4. V. Malenković
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  5. G. Miljuš
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Correspondence to O. Nedić.

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Nedić, O., Šunderić, M., Gligorijević, N. et al. Analysis of four circulating complexes of insulin-like growth factor binding proteins in human blood during aging. Biochemistry Moscow 82, 1200–1206 (2017). https://doi.org/10.1134/S0006297917100133

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  • DOI: https://doi.org/10.1134/S0006297917100133

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