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DNA import into mitochondria

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Abstract

In recent decades, it has become evident that the condition for normal functioning of mitochondria in higher eukaryotes is the presence of membrane transport systems of macromolecules (proteins and nucleic acids). Natural competence of the mitochondria in plants, animals, and yeasts to actively uptake DNA may be directly related to horizontal gene transfer into these organelles occurring at much higher rate compared to the nuclear and chloroplast genomes. However, in contrast with import of proteins and tRNAs, little is known about the biological role and molecular mechanism underlying import of DNA into eukaryotic mitochondria. In this review, we discuss current state of investigations in this area, particularly specificity of DNA import into mitochondria and its features in plants, animals, and yeasts; a tentative mechanism of DNA import across the mitochondrial outer and inner membranes; experimental data evidencing several existing, but not yet fully understood mechanisms of DNA transfer into mitochondria. Currently available data regarding transport of informational macromolecules (DNA, RNA, and proteins) into the mitochondria do not rule out that the mechanism of protein and tRNA import as well as tRNA and DNA import into the mitochondria may partially overlap.

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Abbreviations

ANT:

adenine nucleotide translocator

HGT:

horizontal gene transfer

MPT:

mitochondrial permeability transition

MPTP:

mitochondrial permeability transition pore

mtDNA:

mitochondrial DNA

OXPHOS:

oxidative phosphorylation

PBR/TSPO:

peripheral benzodiazepine receptor/ translocator protein

plDNA:

plasmid-like DNA

VDAC:

voltage-dependent anion channel or mitochondrial porin

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Authors and Affiliations

  1. Siberian Institute of Plant Physiology and Biochemistry, Siberian Branch of the Russian Academy of Sciences, 664033, Irkutsk, Russia

    Yu. M. Konstantinov, E. S. Klimenko, V. I. Tarasenko, T. A. Bolotova & M. V. Koulintchenko

  2. Irkutsk State University, 664003, Irkutsk, Russia

    Yu. M. Konstantinov

  3. Institut de Biologie Moléculaire des Plantes du CNRS, Université Louis Pasteur, 67084, Strasbourg, France

    A. Dietrich, F. Weber-Lotfi, N. Ibrahim & M. V. Koulintchenko

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  1. Yu. M. Konstantinov
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  2. A. Dietrich
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  3. F. Weber-Lotfi
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  4. N. Ibrahim
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  5. E. S. Klimenko
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  7. T. A. Bolotova
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  8. M. V. Koulintchenko
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Correspondence to Yu. M. Konstantinov.

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Original Russian Text © Yu. M. Konstantinov, A. Dietrich, F. Weber-Lotfi, N. Ibrahim, E. S. Klimenko, V. I. Tarasenko, T. A. Bolotova, M. V. Koulintchenko, 2016, published in Biokhimiya, 2016, Vol. 81, No. 10, pp. 1307–1321.

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Konstantinov, Y.M., Dietrich, A., Weber-Lotfi, F. et al. DNA import into mitochondria. Biochemistry Moscow 81, 1044–1056 (2016). https://doi.org/10.1134/S0006297916100035

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