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Cytochrome c–cardiolipin complex in a nonpolar environment

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Abstract

Programmed cell death (apoptosis) plays an important role in the life of multicellular organisms and in the development of socially significant human diseases. Cytochrome c–cardiolipin complex (Cyt-CL) is formed at the very beginning of a cascade of apoptotic reactions. Nevertheless, the structure of the complex and the mechanism of its participation in lipid peroxidation in mitochondrial membranes are not yet understood. In previous work (Vladimirov, Y. A., et al. (2011) Crystallography, 56, 712-719), it was shown that the Cyt-CL complex precipitates in concentrated water solution, the sediment containing orderly nanospheres formed by cytochrome c molecules with changed conformation and surrounded by a cardiolipin monolayer, and they are essentially hydrophobic. In this work, we obtained chloroform and hexane solutions of Cyt-CL with lipid/protein ratio of 77 ± 11. The conditions are described under which the solutions were obtained. Study of the properties of Cyt-CL solutions in hydrophobic media will reveal their structure and the mechanism of their catalytic activity inside the lipid layer of biological membranes.

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Abbreviations

Cyt-CL:

cytochrome c-cardiolipin complex

TOCL:

1,1′,2,2′-tetraoleoylcardiolipin

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Authors and Affiliations

  1. Lomonosov Moscow State University, 119991, Moscow, Russia

    A. S. Vikulina, A. V. Alekseev, E. V. Proskurnina & Yu. A. Vladimirov

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  1. A. S. Vikulina
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  2. A. V. Alekseev
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  3. E. V. Proskurnina
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  4. Yu. A. Vladimirov
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Correspondence to Yu. A. Vladimirov.

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Original Russian Text © A. S. Vikulina, A. V. Alekseev, E. V. Proskurnina, Yu. A. Vladimirov, 2015, published in Biokhimiya, 2015, Vol. 80, No. 10, pp. 1572-1577.

Originally published in Biochemistry (Moscow) On-Line Papers in Press, as Manuscript BM15-157, August 23, 2015.

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Vikulina, A.S., Alekseev, A.V., Proskurnina, E.V. et al. Cytochrome c–cardiolipin complex in a nonpolar environment. Biochemistry Moscow 80, 1298–1302 (2015). https://doi.org/10.1134/S0006297915100107

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  • DOI: https://doi.org/10.1134/S0006297915100107

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