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The Bactericidal and Antibiofilm Effects of New Liposomes Containing Vancomycin Formulation Against Clinical Biofilm Positive Staphylococcus aureus Isolates

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Abstract

The aim of present study was to prepare liposomes containing vancomycin (Lipo-VAN) formulation, and to evaluate the anti-biofilm effect of Lipo-VAN on the expression of icaA genes in clinical strains of Staphylococcus aureus. Lipo-VAN was synthesized using thin-layer hydration method. Formulation no. 3 (F3) with the lowest size of 178.1 nm and polydispersity index value of 0.163 and with the highest efficiency for entrapping drug (65.49%) was selected as an optimal formulation. The formulations were tested for bactericidal and antibiofilm effects against S. aureus strains. The MIC and MBC obtained for the Lipo-VAN against clinical strains were 7.81–62.5 and 15.62–125 µg/mL, respectively. In addition, the reduction in biofilm formation in strains treated with Lipo-VAN was more significant rather than in the VAN-treated and control groups, which was demonstrated with down-regulation of icaA gene expression. Lipo-VAN formulation exhibited elevated antimicrobial and anti-biofilm activity against clinical S. aureus strains.

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Authors and Affiliations

  1. Department of Microbiology, Tehran North Branch, Islamic Azad University, Tehran, Iran

    M. Shiri

  2. Department of Biology, Tehran North Branch, Islamic Azad University, Tehran, Iran

    F. Ashrafi

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  1. M. Shiri
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  2. F. Ashrafi
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Correspondence to F. Ashrafi.

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Shiri, M., Ashrafi, F. The Bactericidal and Antibiofilm Effects of New Liposomes Containing Vancomycin Formulation Against Clinical Biofilm Positive Staphylococcus aureus Isolates. Appl Biochem Microbiol 59, 824–832 (2023). https://doi.org/10.1134/S0003683823060157

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