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Phage Antibodies as Bioreceptors for Ampicillin Detection

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Abstract

Ampicillin-specific antibodies were obtained using a sheep display library of scFv fragments (Griffin.1, UK). Ampicillin was determined by dot-immunoassay with biospecific interaction of selected recombinant antibodies in phage format (phage antibodies). It has been established that the phage antibodies are specific for ampicillin and do not interact with other antibiotics as tetracycline and kanamycin, as well as with substances similar in chemical formula, that is, L-phenylalanine, L-tryptophan, and L-cysteine. It has been shown that the method of solid-phase immunoassay with visual consideration of the results makes detection of ampicillin with the minimum concentration of 1 μg/mL possible. Phage antibodies are promising for use as a sensitive (recognizing) element of sensory systems at the ampicillin detection.

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Funding

The study was supported by the Russian Science Foundation grant No. 22-24-00417, https://rscf.ru/project/22-24-00417/.

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Authors and Affiliations

  1. Institute of Biochemistry and Physiology of Plants and Microorganisms, Subdivision of the Federal State Budgetary Research Institution Saratov Federal Scientific Center of the Russian Academy of Sciences (IBPPM RAS), 410049, Saratov, Russia

    O. I. Guliy, A. S. Fomin, K. P. Gabalov, S. A. Staroverov & O. A. Karavaeva

  2. Chernyshevsky National Research State University, 410012, Saratov, Russia

    A. K. M. Alsowaidi

  3. Saratov State Vavilov Agrarian University, 410012, Saratov, Russia

    S. A. Staroverov

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  1. O. I. Guliy
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  2. A. K. M. Alsowaidi
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  3. A. S. Fomin
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  4. K. P. Gabalov
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  5. S. A. Staroverov
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  6. O. A. Karavaeva
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Correspondence to O. I. Guliy.

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The authors declare they have no conflicts of interest. This work does not contain a description of any studies using humans and animals as subjects.

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Guliy, O.I., Alsowaidi, A.K., Fomin, A.S. et al. Phage Antibodies as Bioreceptors for Ampicillin Detection. Appl Biochem Microbiol 58, 646–651 (2022). https://doi.org/10.1134/S0003683822050088

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  • DOI: https://doi.org/10.1134/S0003683822050088

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