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Bacterial Cellulose as a Matrix for Microorganisms in Bioelectrocatalytic Systems

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Abstract

Bacterial cellulose (BC) produced by the Komagateibacter sucrofermentas VKPM B-11267 bacteria was used as a carrier for immobilization of acetic acid bacteria Gluconobacter oxydans in amperometric biosensors. The bioreceptor was formed on the surface of a screen-printed graphite electrode modified with thermally expanded graphite (TEG) or on the surface of a porous three-dimensional material, nickel foam (NF). Structural features of these materials contributed to the creation of a firm contact between the electrode material and the surface of the BC on which the bacterial cells were immobilized. Scanning electron microscopy showed that bacteria not only sorb on the surface of BC but are also able to penetrate the inner volume of the film. Conductivity of both types of biosensors was studied using impedance spectroscopy and the resistance of the graphite electrode was shown to decrease by three orders of magnitude after its surface is modified with TEG. Bioelectrodes containing BC were used in the construction of an amperometric biosensor for glucose determination. The sensitivity of the biosensor was 3 μA/mM × cm2. Thus, BC in combination with TEG and NF can be used to create three-dimensional electrodes of bioelectrocatalytical devices.

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Funding

The study was carried out within a government task of the Ministry of Education and Science of the Russian Federation no. FEWG-2020-008 and government task of Kotelnikov Institute of Radioengineering and Electronics of RAS no. FFWZ-2022-0002.

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Authors and Affiliations

  1. Institute of Biochemistry and Physiology of Microorganisms, Federal Research Center, Pushchino Biological Research Center, Russian Academy of Sciences, 142290, Pushchino, Moscow oblast, Russia

    S. E. Tarasov, Yu. V. Plekhanova, A. E. Kitova, A. G. Bykov, A. V. Machulin & A. N. Reshetilov

  2. Institute of Radio Engineering and Electronics, Russian Academy of Sciences, 125009, Moscow, Russia

    V. V. Kolesov

  3. Samara National Research University, 443086, Samara, Russia

    N. A. Klenova

  4. National Research Mordovia State University, 430005, Saransk, Russia

    V. V. Revin

  5. Tula State University, 300012, Tula, Russia

    O. N. Ponamoreva & A. N. Reshetilov

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  1. S. E. Tarasov
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  7. N. A. Klenova
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Correspondence to A. N. Reshetilov.

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Translated by A. Bulaev

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Tarasov, S.E., Plekhanova, Y.V., Kitova, A.E. et al. Bacterial Cellulose as a Matrix for Microorganisms in Bioelectrocatalytic Systems. Appl Biochem Microbiol 58, 468–477 (2022). https://doi.org/10.1134/S0003683822040159

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