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Impact of Transcriptional Regulation by Crp, FruR, FlhD, and TyrR on L-tryptophan Biosynthesis in Escherichia coli

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Abstract

Transcriptional regulation plays a pivotal role in metabolic flow, which can, in turn, affect L-Trp biosynthesis in Escherichia coli. The application of global transcriptional regulation by Crp, FruR, FlhD, and TyrR for L-Trp biosynthesis during shake-flask fermentation of E. coli FB-04/pSV03, an L-Trp-producing strain, was elucidated. Inactivation of TyrR was phenotypically silent, whereas, absence of the Crp caused a noticeable reduction in L-Trp yield and a pronounced slow-growth phenotype. Lacking FlhD led to a decrease in the acetate level; however, the L-Trp yield also decreased. When FruR was inactivated, the L-Trp biosynthesis and the biotransformation rate were improved by 59.5 and 52.4%, respectively. However, using the same strain with deleted fruR-gene, for fed-batch cultivation in a 3-L reactor leads to a significant decrease in L-Trp biosynthesis and the conversion rate.

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Funding

This work was supported by financial support from the Natural Science Foundation of Jiangsu Province (BK20170459), China; the National Natural Science Foundation of China (21808075).

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Authors and Affiliations

  1. School of Life Science and Food Engineering, Huaiyin Institute of Technology, 223003, Huaian, China

    L. Liu, M. Bilal & H. Luo

  2. Tecnologico de Monterrey, School of Engineering and Sciences, 64849, Monterrey, Mexico

    H. M. N. Iqbal

Authors
  1. L. Liu
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  2. M. Bilal
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  3. H. Luo
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  4. H. M. N. Iqbal
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Correspondence to L. Liu or M. Bilal.

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The authors report no conflict of interest in any capacity, i.e., competing or financial. This article does not contain any studies involving animals or human participants performed by any of the authors.

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Liu, L., Bilal, M., Luo, H. et al. Impact of Transcriptional Regulation by Crp, FruR, FlhD, and TyrR on L-tryptophan Biosynthesis in Escherichia coli . Appl Biochem Microbiol 57, 319–326 (2021). https://doi.org/10.1134/S0003683821030091

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  • DOI: https://doi.org/10.1134/S0003683821030091

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