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Development of an Immunoenzyme Assay to Control the Total Content of Antibiotics of the Fluoroquinolone Group in Milk

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Abstract

A method for preparing antibodies that specifically recognize antibiotics of the fluoroquinolone group with the same radical in the first position of the quinolone nucleus is proposed. The specificity of rabbit antisera prepared at different cycles of immunization by changing the structure of the hapten in the composition of immunogens was characterized. The selected antibodies provided a group-specific analysis of 16 representatives of fluoroquinolones, including a combination of the following compounds that are controlled in animal products: ciprofloxacin, norfloxacin, pefloxacin, ofloxacin, and enrofloxacin. Using these antibodies, an indirect competitive enzyme-linked immunosorbent assay was developed that was characterized by a detection limit of ciprofloxacin of 0.2 ng/mL and a duration of 2 h. The assay was approbated for the detection of fluoroquinolones in milk.

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REFERENCES

  1. Ezelarab, H.A.A., Abbas, S.H., Hassan, H.A., and Abuo-Rahma, G.E.A., Arch. Pharm. (Weinheim), 2018, vol. 351, no. 9. e1800141.

    Article  Google Scholar 

  2. Blondeau, J.M., Surv. Ophthalmol., 2004, vol. 49, no. 2, pp. 73–S78.

    Article  Google Scholar 

  3. Gouvea, R., Dos, S.F.F., Aquino, M.H.C.D., and Pereira, V.L.D., Bras. J. Poultry Sci., 2015, vol. 17, no. 1, pp. 1–10.

    Article  Google Scholar 

  4. Brown, S.A., J. Vet. Pharm. Therapeutics, 1996, vol. 19, no. 1, pp. 1–14.

    Article  CAS  Google Scholar 

  5. Stahlmann, R. and Lode, H.M., Expert Opin. Drug Saf., 2013, vol. 12, no. 4, pp. 497–505.

    Article  CAS  Google Scholar 

  6. Council Regulation (EEC) No 2377/90 of 26 June 1990 laying down a Community procedure for the establishment of maximum residue limits of veterinary medicinal products in foodstuffs of animal origin.

  7. Uniform sanitary-epidemiological and hygienic requirements for goods subject to sanitary-epidemiological supervision (control). Approved by the Decision of the Commission of the Customs Union dated May 28, 2010, no. 299.

  8. De, A.K., Bera, A.K., and Pal, B., Int. J. Pharm. Sci. Res., vol. 7, no. 2, pp. 531–542.

  9. Sousa, J., Alves, G., Fortuna, A., and Falcao, A., Anal. Bioanal. Chem., 2012, vol. 403, no. 1, pp. 93–129.

    Article  CAS  Google Scholar 

  10. Lehotay, S.J. and Chen, Y.B., Anal. Bioanal. Chem., 2018, vol. 410, no. 22, pp. 5331–5351.

    Article  CAS  Google Scholar 

  11. Xu, F., Ren, K., Yang, Y.Z., Guo, J.P., Ma, G.P., Liu, Y.M., Lu, Y.Q., and Li, X.B., J. Integr. Agricult., 2015, vol. 14, no. 11, pp. 2282–2295.

    Article  CAS  Google Scholar 

  12. Duffy, G.F. and Moore, E.J., Anal. Lett., 2017, vol. 50, no. 1, pp. 1–32.

    Article  CAS  Google Scholar 

  13. Gaudin, V., Biosens. Bioelectron., 2017, vol. 90, pp. 363–377.

    Article  CAS  Google Scholar 

  14. Dzantiev, B.B., Byzova, N.A., Urusov, A.E., and Zherdev, A.V., TrAC, Trends Anal. Chem., 2014, vol. 55, pp. 81–93.

  15. Tittlemier, S., Gelinas, J.-M., Dufresne, G., Haria, M., Querry, J., Cleroux, C., and Godefroy, S.B., Food Anal. Methods, 2008, vol. 1, no. 1, pp. 28–35.

    Article  Google Scholar 

  16. Huet, A.-C., Charelier, C., Tittlemier, S.A., Singh, G., Benrejeb, S., and Delahaut, P., J. Agric. Food Chem., 2006, vol. 54, no. 8, pp. 2822–2827.

    Article  CAS  Google Scholar 

  17. Liu, Y.Z., Zhao, G.X., Wang, P., Liu, J., Zhang, H.C., and Wang, J.P., J. Environ. Sci. Health, 2013, vol. 48, no. 2, pp. 139–146.

    Article  CAS  Google Scholar 

  18. Li, Y., Ji, B., Chen, W., Liu, L., Xu, C., Peng, C., and Wang, L., Food Agric. Immunol., 2008, vol. 19, no. 4, pp. 251–264.

    Article  Google Scholar 

  19. Wang, Z., Zhu, Y., Ding, S., He, F., Beier, R.C., Li, J., and Shen, J., Anal. Chem., 2007, vol. 79, no. 12, pp. 4471–4483.

    Article  CAS  Google Scholar 

  20. Bucknall, S., Silverlight, J., Coldham, N., Thorne, L., and Jackman, R., Food Additives Contam., 2003, vol. 3, no. 3, pp. 221–228.

    Article  Google Scholar 

  21. Burkin, M.A., Food Agric. Immunol., 2008, vol. 19, no. 2, pp. 131–140.

    Article  CAS  Google Scholar 

  22. Wen, K., Nolke, G., Schillberg, S., Wang, Z., Zhang, S., Wu, C., and Shen, J., Anal. Bioanal. Chem., 2012, vol. 403, no. 9, pp. 2771–2783.

    Article  CAS  Google Scholar 

  23. Lu, S., Zhang, Y., Liu, J., Zhao, C., Liu, W., and Xi, R., J. Agric. Food Chem., 2006, vol. 54, no. 19, pp. 6995–7000.

    Article  CAS  Google Scholar 

  24. Chu, F.S., Lau, H.P., Fan, T.S., and Zhang, G.S., J. Immunol. Methods, 1982, vol. 55, no. 3, pp. 73–78.

    Article  CAS  Google Scholar 

  25. Liu, Z.Q., Lu, S.X., Zhao, C.H., Ding, K., Cao, Z.Z., Zhan, J.H., Ma, C., Liu, J.T., and Xi, R.M., J. Sci. Food Agric., 2009, vol. 89, no. 7, pp. 1115–1121.

    Article  CAS  Google Scholar 

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Funding

The work was performed with partial financial support from the Russian Foundation for Basic Research (Grant no. 18-58-00038) and the Belarusian Republican Foundation for Fundamental Research (Grant no. H18P-060).

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Authors and Affiliations

  1. Lomonosov Moscow State University, 119991, Moscow, Russia

    I. A. Shanin & S. A. Eremin

  2. “Xema”, 125319, Moscow, Russia

    I. A. Shanin

  3. Bach Institute of Biochemistry, Research Center of Biotechnology of the Russian Academy of Sciences, 119071, Moscow, Russia

    E. A. Zvereva, A. V. Zherdev & B. B. Dzantiev

  4. Institute of Bioorganic Chemistry of the National Academy of Sciences of Belarus, 220141, Minsk, Belarus

    O. V. Sviridov

Authors
  1. I. A. Shanin
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  2. E. A. Zvereva
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  3. S. A. Eremin
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  4. O. V. Sviridov
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  5. A. V. Zherdev
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  6. B. B. Dzantiev
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Corresponding author

Correspondence to B. B. Dzantiev.

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The authors declare that they have no conflict of interest. This article does not contain any studies involving animals or human participants performed by any of the authors.

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Shanin, I.A., Zvereva, E.A., Eremin, S.A. et al. Development of an Immunoenzyme Assay to Control the Total Content of Antibiotics of the Fluoroquinolone Group in Milk. Appl Biochem Microbiol 55, 563–569 (2019). https://doi.org/10.1134/S0003683819050132

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  • DOI: https://doi.org/10.1134/S0003683819050132

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