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Isolation and Characterization of Toxic Cyanobacteria from Different Natural Sources

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Abstract

We isolated seven algologically and five bacteriologically pure cultures of toxin-producing cyanobacteria from Turgen gorge (Kazakhstan), Karlovy Vary (Czech Republic), and Shar-Nuur Lake, Bayan Ulgiiregion (Mongolia) springs. According to the Daphnia magna test, Desertifilum sp. and Nostoc sp. strains were the most toxic in the test of isolated strains (complete death of all test organisms was detected after 48 h). These strains possessed the highest inhibitory effect on proliferation of the HeLa cancer cell line. The Anabaena sp. 35 and Nostoc sp. 4 strains were also high toxic. Model strains Synechocystis PCC 6803 and Synechococcus elongates PCC 7942, as well as the strain isolated in the present work, Synechococcus sp. 55, were less toxic. Mass spectrometry made it possible to assign cyanobacterial toxins to cyclic depsipeptides. Two cyclic depsipeptides, micropeptin T and oscillapeptin, were detected in Desertifilum sp. extracts. Cryptophycin and small amounts of cyclic depsipeptide micropeptin SD were detected in Nostoc sp. extract.

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Abbreviations

HPLC:

high-performance liquid chromatography

MSI:

ionized molecules

m/z :

mass-charge

DA:

ultraviolet polychromatic detector

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Authors and Affiliations

  1. Al-Farabi Kazakh National University, Almaty, 480078, Kazakhstan

    K. Bolatkhan, N. R. Akmukhanova, B. K. Zayadan & A. K. Sadvakasova

  2. Timiryazev Institute for Plant Physiology, Russian Academy of Sciences, Moscow, 127276, Russia

    M. A. Sinetova & D. A. Los

Authors
  1. K. Bolatkhan
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  2. N. R. Akmukhanova
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  3. B. K. Zayadan
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  4. A. K. Sadvakasova
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  5. M. A. Sinetova
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  6. D. A. Los
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Correspondence to K. Bolatkhan or B. K. Zayadan.

Additional information

Original Russian Text © K. Bolatkhan, N.R. Akmukhanova, B.K. Zayadan, A.K. Sadvakasova, M.A. Sinetova, D.A. Los, 2016, published in Biotekhnologiya, 2016, Vol. 32, No. 3, pp. 57–66.

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Bolatkhan, K., Akmukhanova, N.R., Zayadan, B.K. et al. Isolation and Characterization of Toxic Cyanobacteria from Different Natural Sources. Appl Biochem Microbiol 53, 754–760 (2017). https://doi.org/10.1134/S000368381707002X

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  • DOI: https://doi.org/10.1134/S000368381707002X

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