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Evaluation of a method for the determination of antibacterial activity of chitosan

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Abstract

A method for the determination of the antimicrobial activity of chitosan with the use of organic salts for the production of pH in the range of 5.5–8.2 was studied. The double-dilution method demonstrated the effectiveness of the determination of the antimicrobial activity of chitosan samples with different molecular weights and solubilities. It was found that the antibacterial activity increased at low pH values with increasing molecular weight, but chitosans with a molecular weight of 5–6 kDa showed higher activity at neutral and slightly alkaline pH levels. Determination of the antimicrobial activity of various chitosan samples at different pH values allowed a more reliable assessment of the potential biological activity of chitosan.

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Authors and Affiliations

  1. Kazan Scientific Research Institute of Epidemiology and Microbiology at Rospotrebnadzor, Kazan, 420015, Russia

    S. N. Kulikov, L. T. Bayazitova & O. F. Tyupkina

  2. Kazan Federal University, Kazan, 420008, Russia

    S. N. Kulikov, P. V. Zelenikhin & M. M. Salnikova

  3. A.N. Nesmeyanov Institute of Organoelement Compounds, Russian Academy of Sciences (IOS RAS), Moscow, 117312, Russia

    E. A. Bezrodnykh & V. E. Tikhonov

Authors
  1. S. N. Kulikov
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  2. L. T. Bayazitova
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  3. O. F. Tyupkina
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  4. P. V. Zelenikhin
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  5. M. M. Salnikova
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  6. E. A. Bezrodnykh
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  7. V. E. Tikhonov
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Corresponding author

Correspondence to S. N. Kulikov.

Additional information

Original Russian Text © S.N. Kulikov, L.T. Bayazitova, O.F. Tyupkina, P.V. Zelenikhin, M.M. Salnikova, E.A. Bezrodnykh, V.E. Tikhonov, 2016, published in Prikladnaya Biokhimiya i Mikrobiologiya, 2016, Vol. 52, No. 5, pp. 489–494.

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Kulikov, S.N., Bayazitova, L.T., Tyupkina, O.F. et al. Evaluation of a method for the determination of antibacterial activity of chitosan. Appl Biochem Microbiol 52, 502–507 (2016). https://doi.org/10.1134/S0003683816050100

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  • DOI: https://doi.org/10.1134/S0003683816050100

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