Abstract
The dependence of changes in the electrooptical properties of Azospirillum brasilense cell suspension Sp7 during interaction with bacteriophage ΦAb-Sp7 on the number and time of interactions was studied. Incubation of cells with bacteriophage significantly changed the electrooptical signal within one minute. The selective effect of bacteriophage ΦAb on 18 strains of bacteria of the genus Azospirillum was studied: A. amazonense Am1i4, A. brasilense Sp7, Cd, Spl07, Sp245, Jm6B2, Brl4, KR77, S17, S27, SR55, SR75, A. halopraeferans Au4, A. irakense KBC1, KA3, A. lipoferum Sp59b, SR65 and RG20a. We determined the limit of reliable determination of microbial cells infected with bacteriophage: ∼104 cells/mL. The presence of foreign cell cultures of E. coli B-878 and E. coli XL-1 did not complicate the detection of A brasilense Sp7 cells with the use of bacteriophage ΦAb-Sp7. The results demonstrated that bacteriophage ΦAb-Sp7 can be used for the detection of Azospirillum microbial cells by the electrooptical analysis of cell suspensions.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Steenhoudt, O. and Vanderleyden, J., FEMS Microbiol. Lett., 2000, vol. 24, no. 4, pp. 487–506.
Bashan, Y., Can. J. Microbiol., 1998, vol. 44, no. 2, pp. 168–174.
Molekulyarnye osnovy vzaimodeistviya assotsiativnykh mikroorganizmov s rasteniyami (Molecular Basis of Interaction of Associative Microorganisms with Plants), Ignatov, V.V., Ed., Moscow: Nauka, 2005.
Baldani, J.I. and Baldani, V.L., Acad. Bras. Cienc., 2005, vol. 77, no. 3, pp. 549–579.
Mulyukin, A.L., Suzina, N.E., Pogorelova, A.Yu., Antonyuk, L.P., Duda, V.I., and El’-Registan, G.I., Microbiology (Moscow), 2009, vol. 78, no. 1, pp. 33–41.
Krasov, A.I., Popova, I.A., Filip’echeva, Yu.A., Burygin, G.L., and Matora, L.Yu., Microbiology (Moscow), 2009, vol. 78, no. 5, pp. 598–602.
Schmelcher, M. and Loessner, M., in Principles of Bacterial Detection: Biosensors, Recognition Receptors and Microsystems, Zourob, M., Elwary, S., and Turner, A., Eds., New York: Springer Science, Business Media, LLC, 2008, pp. 731–754.
Ripp, S., Adv. Biochem. Engin. Biotechnol., 2010, vol. 118, pp. 65–84.
Singh, A., Arutyunov, D., Szymanski, C.M., and Evoy, S., Analyst, 2012, vol. 137, no. 15, pp. 3405–3421.
Vaughan, R.D., O’Sullivan, C.K., and Cuilbault, G.G., Enzyme Microb. Technol., 2001, vol. 29, no. 10, pp. 635–638.
Zaitsev, B.D., Kuznetsova, I.E., Shikhabudinov, A.M., Ignatov, O.V., and Guliy, O.I., IEEE Trans. Ultrason Ferroelectr. Freq. Control, 2012, vol. 59, no. 5, pp. 963–969.
Smartt, A.E. and Ripp, S., Anal. Bioanal. Chem., 2011, vol. 400, no. 4, pp. 991–1007.
Ignatov, O.V., Guliy, O.I., and Bunin, V.D., in Commercial and Pre-commercial Cell Detection Technologies for Defense against Bioterror-Technology, Market and Society, Lechuga, L.M., Milanovich, F.P., Skladal, P., Ignatov, O.V., and Austin, T., Eds., Amsterdam: IOS Press, 2008, pp. 45–53.
Gulii, O.I., Matora, L.Yu., Burygin, G.L., Dykman, L.A., Ignatov, V.V., and Ignatov, O.V., Appl. Biochem. Microbiol., 2010, vol. 46, no. 1, pp. 61–64.
Shchyogolev, S.Y., Khlebtsov, N.G., Bunin, V.D., Sirota, A.I., and Bogatyrev, V.A., in Inverse Problems of Spectroturbidimetry of Biological Disperse Systems with Random and Ordered Particle Orientation, Chance, B., Delpy, D.T., Ferrari, M., van Gemert, M.J. C., Mueller, G.J., and Tuchin, V.V., Eds., Bellingham, Washington: SPIE, 1994, pp. 167–176.
Guliy, O.I., Matora, L.Yu., Burygin, G.L., Dykman, L.A., Ostudin, N.A., Bunin, V.D., Ignatov, V.V., and Ignatov, O.V., Anal. Biochem., 2007, vol. 370, no. 2, pp. 201–205.
Gulii, O.I., Bunin, V.D., Neil, D., Ivnitskii, D., Matora, L.Yu., Ignatov, V.V., and Ignatov, O.V., Zh. Mikrobiol. Epidemiol. Immunol., 2007, no. 5, pp. 80–82.
Bunin, V.D., Ignatov, O.V., Gulii, O.I., Zaitseva, I.S., Neil, D., and Ivnitskii, D., Microbiology (Moscow), 2005, vol. 74, no. 2, pp. 164–168.
Sambrook, J., in Molecular Cloning: A Laboratory Manual, Sambrook, J., Fritsch, E.F., Maniatis, T., Eds., New York: Cold Spring Harbor Laboratory Press, 1982.
Ignatov, O.V., Guliy, O.I., Shchyogolev, S.Yu., Bunin, V.D., and Ignatov, V.V., FEMS Microbiol. Letts., 2002, vol. 214, no. 1, pp. 81–86.
Elmerich, C., Quiviger, B., Rosenberg, C., Franche, C., Laurent, P., and Döbereiner, J., Virology, 1982, vol. 122, no. 1, pp. 29–37.
MC, GreenP., Genome Res., 1998, vol. 8, no. 3, pp. 175–185.
Germida, J.J., Can. J. Microbiol., 1984, vol. 30, no. 6, pp. 805–808.
Boyer, M., Haurat, J., Samain, S., Segurens, B., Gavory, F., González, V., Mavingui, P., Rohr, R., Bally, R., and Wisniewski-Dyé, F., Appl. Environ. Microbiol., 2008, vol. 74, no. 3, pp. 861–874.
Adams, M., Bakteriofagi (Bacteriophages), Adams, M., Ed., Moscow: Medgiz, 1961.
Labinskaya, A.S., Mikrobiologiya s tekhnikoi mikrobiologicheskikh issledovanii (Microbiology with the Technique of Microbiological Tests), Efremova, S.A, Ed., Moscow: Meditsina, 1978.
Author information
Authors and Affiliations
Corresponding author
Additional information
Original Russian Text © O.I. Guliy, O.A. Karavaeva, S.A. Pavliy, O.I. Sokolov, V.D. Bunin, O.V. Ignatov, 2015, published in Prikladnaya Biokhimiya i Mikrobiologiya, 2015, Vol. 51, No. 3, pp. 313–318.
Rights and permissions
About this article
Cite this article
Guliy, O.I., Karavaeva, O.A., Pavliy, S.A. et al. Determination of Azospirillum brasilense cells with bacteriophages by electrooptical analysis of microbial suspensions. Appl Biochem Microbiol 51, 316–320 (2015). https://doi.org/10.1134/S0003683815030084
Received:
Published:
Issue Date:
DOI: https://doi.org/10.1134/S0003683815030084