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Genetic and molecular principles for the selection of Pseudomonas and Staphylococcus therapeutic bacteriophages

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Abstract

The content of empirically selected bacteriophage mixtures, produced by Microgen for the prevention and treatment of staphylococcal and pseudomonade infections, was investigated by negative stain electron microscopy. The main population of phages was shown to belong to the groups suitable for therapeutic purposes based on bioinformatics analysis of known genomes of Pseudomonas and Staphylococcus phages. However, the phage morphology studies did not always reveal the exact correspondence of the phage to the exact group. Therefore, we suggest group genotyping of the therapeutic bacteriophages on the basis of genetic conservative locus.

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Authors and Affiliations

  1. Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, ul. Miklukho-Maklaya 16/10, Moscow, 117997, Russia

    K. A. Miroshnikov

  2. Winogradsky Institute of Microbiology, Russian Academy of Sciences, pr. 60-letiya Oktyabrya 7, k. 2, Moscow, 117312, Russia

    E. E. Kulikov

  3. Scientific Center for Expert Evaluation of Medicinal Products, Ministry of Healthcare of the Russian Federation, Petrovskii bulv. 8, 127051, Moscow, Russia

    O. S. Darbeeva

  4. Federal State Unitary Research and Production Association “Microgen”, Ministry of Healthcare of the Russian Federation, Vtoroi Volkonskii per. 10, Moscow, 127473, Russia

    K. A. Lysko & G. M. Ignatyev

Authors
  1. K. A. Miroshnikov
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  2. E. E. Kulikov
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  3. O. S. Darbeeva
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  4. K. A. Lysko
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  5. G. M. Ignatyev
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Correspondence to K. A. Miroshnikov.

Additional information

Original Russian Text © K.A. Miroshnikov, E.E. Kulikov, O.S. Darbeeva, K.A. Lysko, G.M. Ignatyev, 2014, published in Prikladnaya Biokhimiya i Mikrobiologiya, 2014, Vol. 50, No. 3, pp. 338–344.

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Miroshnikov, K.A., Kulikov, E.E., Darbeeva, O.S. et al. Genetic and molecular principles for the selection of Pseudomonas and Staphylococcus therapeutic bacteriophages. Appl Biochem Microbiol 50, 306–312 (2014). https://doi.org/10.1134/S0003683814030090

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  • DOI: https://doi.org/10.1134/S0003683814030090

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