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Effects of native and oxidized apolipoprotein A-I on lipid bilayer microviscosity of erythrocyte plasma membrane

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Biochemistry (Moscow), Supplement Series A: Membrane and Cell Biology Aims and scope

Abstract

Using a pyrene as a fluorescent probe, we investigated the influence of native and oxidized apolipoprotein A-I (apo A-I) and their complexes with tetrahydrocortisol (THC) on the microviscosity of the erythrocyte plasma membrane. The addition of THC to isolated membranes led to a 17% increase in the membrane microviscosity. In contrast, native apo A-I reduced the microviscosity (i.e., increased the fluidity) of the membranes by 15%. A more pronounced increase (by 25%) in the membrane fluidity was found in the presence of the complex of apo A-I with THC. Unlike native apo A-I, oxidized apo A-I and its complex with THC did not change the membrane viscosity. In view of the fact that apo A-I plays an important role in the binding of membrane cholesterol we suggest that the observed increase in the membrane fluidity under the influence of the native apo A-I is associated with the cholesterol efflux from plasma membrane. Oxidative modification of apo A-I likely disturbs the mechanisms of the cholesterol efflux and prevents the decrease in the membrane microviscosity.

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References

  1. Koike T., Ishida G., Taniguchi M., Higaki K., Ayaki Y., Saito M., Sakakihara Y., Iwamori M., Ohno K. 1998. Decreased membrane fluidity and unsaturated fatty acids in Niemann–Pick disease type C fibroblasts. Biochim. Biophys. Acta. 1406, 327–335.

    Article  CAS  PubMed  Google Scholar 

  2. Osterode W., Holler C., Ulberth F. 1996. Nutritional antioxidants, red cell membrane fluidity and blood viscosity in type 1 (insulin dependent) diabetes mellitus. Diabet. Med. 13, 104–105.

    Article  Google Scholar 

  3. Gleason M.M., Medow M.S., TulenkoT.N. 1991. Excess membrane cholesterol alters calcium movements, cytosolic calcium levels, and membrane fluidity in arterial smooth muscle cells. Circ. Res. 69, 216–227.

    Article  CAS  Google Scholar 

  4. Zubenko G.S., Kopp U., Seto T., Firestone L.L. 1999. Platelet membrane fluidity individuals at risk for Alzheimer’s disease: A comparison of results from fluorescence spectroscopy and electron spin resonance spectroscopy. Psychopharmacology (Berl.). 145, 175–180.

    Article  CAS  PubMed  Google Scholar 

  5. Chabanel A., Flamm M., Sung K.L., Lee M.M., Schachter D., Chien S. 1983. Influence of cholesterol content on red cell membrane viscoelasticity and fluidity. Biophys. J. 44 (2), 171–176.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  6. Borovskaya M.K., Kuznetsova E.E., Gorokhova V.G., Koryakina L.B., Kurilskaya T.E., Pivovarov Y.I. 2010. Structural and functional characteristics of the erythrocyte membrane and its changes in pathologies of different genesis. Bull. VSNTs SBRAMS (Rus.). 3 (73), 334–354.

    Google Scholar 

  7. Panin L.E., Mokrushnikov P.V. 2014. The action of androgens on Na+,K+- ATPase activity of erythrocyte membranes. Biophysics. 59 (1), 127–133.

    Article  Google Scholar 

  8. Mokrushnikov P.V., Panin L.E., Zaitsev B.N. 2015. The action of stress hormones on the structure and function of erythrocyte membrane. Gen. Physiol. Biophys. 34 (3), 311–321.

    CAS  PubMed  Google Scholar 

  9. Takahashi Y., Smith J.D. 1999. Cholesterol efflux to apolipoprotein AI involves endocytosis and resecretion in a calcium-dependent pathway. Proc. Natl. Acad. Sci. USA. 96, 11358–11363.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  10. Shao B. 2012. Site-specific oxidation of apolipoprotein A-I impairs cholesterol export by ABCA1, a key cardioprotective function of HDL. Biochim. Biophys. Acta. 1821, 490–501.

    Article  CAS  PubMed  Google Scholar 

  11. Pankhurst G., Wang X.L., Wilcken D.E., Baernthaler G., Panzenbö ck U., Raftery M., Stocker R. 2003. Characterization of specifically oxidized apolipoproteins in mildly oxidized high density lipoprotein. J. Lipid Res. 44 (2), 349–355.

    Article  CAS  PubMed  Google Scholar 

  12. Panin L.E., Usynin I.F., Khar’kovskii A.V. 2000. Effect of tetrahydrocortisol on the protein biosynthesis in hepatocytes. Bull. Exp. Biol. Med. 129 (2), 146–148.

    Article  CAS  Google Scholar 

  13. Panin L.E., Maksimov V.F., Usynin I.F., Korostyshevskaya I.M. 2002. Activation of nucleolar DNA expression in hepatocytes by glucocorticoids and high density lipoproteins. J. Steroid Biochem. Mol. Biol. 81 (1), 69–76.

    Article  CAS  PubMed  Google Scholar 

  14. Panin L.E., Mokrushnikov P.V., Kunitsyn V.G., Zaitsev B.N. 2010. The interaction mechanism of cortisol and catecholamines with structural components of erythrocyte membranes. J. Phys. Chem. B. 114, 9462–9473.

    Article  CAS  PubMed  Google Scholar 

  15. Panin L.E., Mokrushnikov P.V., Kunitsyn V.G., Panin V.E., Zaitsev B.N. 2011. Fundamentals of multilevel mesomechanics of nanostructural transitions in erythrocyte membranes and their destructions in interaction with stress hormones. Phys. Mesomechanics. 14 (3–4), 167–177.

    Article  Google Scholar 

  16. Panin L.E., Mokrushnikov P.V., Kunitsyn V.G., Zaitsev B.N. 2011. Interaction mechanism of anabolic steroid hormones with structural components of erythrocyte membranes. J. Phys. Chem. B. 115, 14969–14979.

    Article  CAS  PubMed  Google Scholar 

  17. Vladimirov Yu.A., Dobretsov G.E. 1980. Fluorestsentnye zondy v issledovanii biologicheskikh membran (Fluorescent probes in the study of biological membranes). M.: Nauka.

    Google Scholar 

  18. Dobretsov G.E. 1989. Fluorestsentnye zondy v issledovanii kletok, membran i lipoproteinov (Fluorescent probes in the study of cells, membranes, and lipoproteins). M.: Nauka.

  19. Hatch F.T., Less R.S. 1968. Practical methods for plasma lipoprotein analysis. Adv. Lipid Res. 6, 2–68.

    Google Scholar 

  20. Cham B.E., Knowles B.R. 1976. A solvent system for delipidation of plasma or serum without protein precipitation. J. Lipid. Res. 17 (2), 176–181.

    CAS  PubMed  Google Scholar 

  21. Pykhtina M.B., Ivanov I.D., Beklemishev A.B. 2012. Development of effective ways of apolipoprotein A-I isolation from human blood plasma. Biofarmakol. Zhurnal (Rus.). 4 (6), 37–45.

    CAS  Google Scholar 

  22. Lowry O.H., Rosebrough N.J., Farr A.L., Randall R.J. 1952. Protein measurement with the folin phenol reagent. J. Biol. Chem. 193, 265–275.

    Google Scholar 

  23. Nagano Y., Arai H., Kita T. 1991. High density lipoprotein loses its effect to stimulate efflux of cholesterol from foam cells after oxidative modification. Proc. Natl. Acad. Sci. USA. 88, 6457–6461.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  24. Chajés V., Lhuillery C., Sattler W., Kostner G.M., Bougnoux P. 1996. Alpha-tocopherol and hydroperoxide content in breast adipose tissue from patients with breast tumors. J. Cancer. 67(2), 170–175.

    Google Scholar 

  25. Stal’naya I.D., Garishvili T.G. 1978. Sovremennye metody v biokhimii (Modern methods in biochemistry). M.: Nauka.

    Google Scholar 

  26. Sata M., Waksh K. 1998. TNF alpha regulation of Fas ligand expression on the vascular endothelium modulates leukocyte extravasation. Nat. Med. 4 (4), 415–420.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  27. Attallah N.A., Lata G.F. 1968. Steroid-protein interactions studied by fluorescence quenching. Biochim. Biophys. Acta. 168, 321–333.

    Article  CAS  PubMed  Google Scholar 

  28. Panin L.E., Butusova V.N., Ryazantseva N.V., Novitskii N.V. 2009. Effects of blood lipoproteins and apolipoproteins A-I, C, and E on microviscosity of erythrocyte membranes. Bull. Exp. Biol. Med. (Rus.). 148 (9), 273–276 [Translated version in: Bull. Exp. Biol. Med. (2009). 148 (3), 385–388. doi 10.1007/s10517-010-0719-4]

    Google Scholar 

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Authors and Affiliations

  1. Research Institute of Biochemistry, ul. Timakova 2, Novosibirsk, 630117, Russia

    P. V. Mokrushnikov, A. N. Dudarev, T. A. Tkachenko, A. Y. Gorodetskaya & I. F. Usynin

  2. Novosibirsk State University of Architecture and Civil Engineering, ul. Leningradskaya 113, Novosibirsk, 630008, Russia

    P. V. Mokrushnikov

Authors
  1. P. V. Mokrushnikov
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  2. A. N. Dudarev
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  3. T. A. Tkachenko
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  4. A. Y. Gorodetskaya
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  5. I. F. Usynin
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Correspondence to P. V. Mokrushnikov.

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Original Russian Text © P.V. Mokrushnikov, A.N. Dudarev, T.A. Tkachenko, A.Y. Gorodetskaya, I.F. Usynin, 2016, published in Biologicheskie Membrany, 2016, Vol. 33, No. 6, pp. 406–411.

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Mokrushnikov, P.V., Dudarev, A.N., Tkachenko, T.A. et al. Effects of native and oxidized apolipoprotein A-I on lipid bilayer microviscosity of erythrocyte plasma membrane. Biochem. Moscow Suppl. Ser. A 11, 48–53 (2017). https://doi.org/10.1134/S1990747816040164

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  • DOI: https://doi.org/10.1134/S1990747816040164

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