Abstract
A new series of heat-stable (st) mutants of bacteriophage T5, which contains deletions in the tRNA gene region, has been isolated. An accurate mapping of the deletion boundaries for more than 30 mutants of phage T5 has been carried out. As a result of the analysis of nucleotide sequences flanking the deleted regions in wild-type phage DNA, it has been shown that they all contain short, direct repeats of different lengths (2–35 nucleotide residues), and that only one repetition is retained in the mutant phage DNA. On the basis of the obtained results, it was suggested that deletion mutants of the phage T5 are formed as a result of illegal recombination occurring with the participation of short repeats in DNA (SHDIR). Based on the example of two mutants, it has been shown that the resistance to thermal inactivation depends on the size of the deleted region.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Shliapnikov M.G., Ksenzenko V.N. 1994. Features of the structure of transfer RNA coded by bacteriophage T5. Mol. Biol. (Moscow). 28, 818–823.
McCorquodale D.J. 1975. The T-odd bacteriophages. CRC Crit. Rev. Microbiol. 4, 101–159.
Rhoades M., Schwartz J., Wahl J.M. 1980. New deletion mutant of bacteriophage T5. J. Virol. 36, 622–626.
Rhoades M. 1982. New physical map of bacteriophage T5 DNA. J. Virol. 43, 566–573.
Chen M.J., Locker J., Weiss S.B. 1976. The physical mapping of bacteriophage T5 transfer tRNAs. J. Biol. Chem. 251, 536–547.
Chernov A.P., Kriukov V.M., Baev A.A. 1979. New series of thermostable phage T5 mutants carrying deletions in the region of the tRNA genes. Genetika. 15, 1724–1729.
Lanni Y.T. 1958. Lysis inhibition with a mutant of bacteriophage T5. Virology. 5, 481–501.
Hertel R., Marchi L., Muller K. 1962. Density mutants of phage T5. Virology. 18, 576–581.
Gergen J.P., Stern R.H., Wensink P.C. 1979. Filter replicas and permanent collections of recombinant DNA plasmids. Nucleic Acids Res. 7, 2115–2136.
Boulanger P. 2009. Purification of bacteriophages and SDS-PAGE analysis of phage structural proteins from ghost particles. Meth. Mol. Biol. 502, 227–238.
Shimizu H., Yamaguchi H., Ashizawa Y., et al. 1997. Short-homology-independent illegitimate recombination in Escherichia coli: Distinct mechanism from short-homology-dependent illegitimate recombination. J. Mol. Biol. 266, 297–305.
Adams M.H., Lark G. 1950. Mutation to heat resistance in coliphage T51. J. Immunol. 64, 335–347.
Vernhes E., Renouard M., Gilquin B., et al. 2017. High affinity anchoring of the decoration protein pb10 onto the bacteriophage T5 capsid. Sci. Rep. 7, 41662–41675.
McCorquodale D.J., Warner H.R. 1988. Bacteriophage T5 and related phages. In: The Bacteriophages. Ed. Calendar R. Boston, MA: Springer, vol. 1, pp. 439–475.
Lang D., Shaw A.R., McCorquodale D.J. 1975. Molecular weights of DNA from bacteriophages T5, T5st(O), BF23, and BF23st(4). J. Virol. 17, 296–297.
Heller K.J., Bryniok D. 1984. O antigen-dependent mutant of bacteriophage T5. J. Virol. 49, 20–25.
Rubenstein I. 1968. Heat-stable mutants of T5 phage: 1. The physical properties of the phage and their DNA molecules. Virology. 36, 356–376.
Mosig G., Gewin J., Luder A., et al. 2001. Two recombination-dependent DNA replication pathways of bacteriophage T4, and their roles in mutagenesis and horizontal gene transfer. Proc. Natl. Acad. Sci. U. S. A. 98, 8306–8311.
Akulenko N.V., Ivashina T.V., Shaloiko L.A., et al. 2004. Novel site-specific endonucleases F-TflI, F-TflII and F-TflIV encoded by the bacteriophage T5. Mol. Biol. (Moscow). 38, 632–641.
Rabsch W., Ma L., Wiley G., et al. 2007. FepA- and TonB-dependent bacteriophage H8: Receptor binding and genomic sequence. J. Bacteriol. 189, 5658–5674.
Golomidova A.K., Kulikov E.E., Prokhorov N.S., et al. 2015. Complete genome sequences of T5-related Escherichia coli bacteriophages DT57C and DT571/2 isolated from horse feces. Arch. Virol. 160, 3133–3137.
Author information
Authors and Affiliations
Corresponding author
Additional information
Original Russian Text © A.S. Glukhov, A.I. Krutilina, A.V. Kaliman, M.G. Shlyapnikov, V.N. Ksenzenko, 2018, published in Molekulyarnaya Biologiya, 2018, Vol. 52, No. 1, pp. 3–9.
Rights and permissions
About this article
Cite this article
Glukhov, A.S., Krutilina, A.I., Kaliman, A.V. et al. Bacteriophage T5 Mutants Carrying Deletions in tRNA Gene Region. Mol Biol 52, 1–6 (2018). https://doi.org/10.1134/S0026893318010065
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1134/S0026893318010065