Abstract
In this paper, we present the results of experimental studies on the influence of different magnesium isotopes, magnetic 25Mg and nonmagnetic 24Mg or 26Mg, on ATP-hydrolytic activity of the isolated myosin subfragment-1. The reaction rate in the presence of magnetic 25Mg isotope turned out to be 2.0–2.5 times higher than that using non-magnetic 24Mg or 26Mg isotopes. In absence of the enzyme, as at spontaneous ATP hydrolysis in aqueous solution, no magnetic isotope effect was observed. Thus, a significant catalytic effect of the magnetic 25Mg isotope (nuclear spin catalysis) was discovered in the enzymatic hydrolysis of ATP.
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Original Russian Text © V.K. Koltover, R.D. Labyntseva, V.K. Karandashev, S.O. Kosterin, 2016, published in Biofizika, 2016, Vol. 61, No. 2, pp. 239–246.
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Koltover, V.K., Labyntseva, R.D., Karandashev, V.K. et al. Magnetic isotope of magnesium accelerates ATP hydrolysis catalyzed by myosin. BIOPHYSICS 61, 200–206 (2016). https://doi.org/10.1134/S0006350916020068
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DOI: https://doi.org/10.1134/S0006350916020068