Abstract
A low-molecular derivative of the polysaccharide (5 kDa) was obtained and its cytokine-inducing and anti-inflammatory activity was studied by free radical depolymerization of chitosan (110 kDa). It was shown that high-molecular chitosan in vitro inhibited the synthesis of anti-inflammatory cytokine, the tumor necrosis factor alpha induced by endotoxin. In the case of peroral introduction to experimental animals, high- and low-molecular chitosans stimulated synthesis of the anti-inflammatory cytokine IL-10 in the blood serum of mice; in this case, the activity of the high-molecular derivative was two times higher as compared with the initial polysaccharide. With peroral introduction, the initial polysaccharide (50 mg/kg) and its derivative inhibited the development of chemically induced inflammation of experimental animals’ large intestines, which was manifested as a decrease in the affected area and the degree of damage to the large intestine wall, as well as a two-fold reduction of myeloperoxidase activity. According to morphological and biochemical characteristics, the effect of chitosans was similar to that of a hormone anti-inflammatory drug, prednisolone.
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Original Russian Text © V.N. Davydova, A.A. Kalitnik, P.A. Markov, A.V. Volod’ko, S.V. Popov, I.M. Ermak, 2016, published in Prikladnaya Biokhimiya i Mikrobiologiya, 2016, Vol. 52, No. 5, pp. 460–466.
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Davydova, V.N., Kalitnik, A.A., Markov, P.A. et al. Cytokine-inducing and anti-inflammatory activity of chitosan and its low-molecular derivative. Appl Biochem Microbiol 52, 476–482 (2016). https://doi.org/10.1134/S0003683816050070
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DOI: https://doi.org/10.1134/S0003683816050070