Abstract
Auditory and vestibular hair cell bundles exhibit active mechanical oscillations at natural frequencies that are typically lower than the detection range of the corresponding end organs. We explore how these noisy nonlinear oscillators mode-lock to frequencies higher than their internal clocks. A nanomagnetic technique is used to stimulate the bundles without an imposed mechanical load. The evoked response shows regimes of high-order mode-locking. Exploring a broad range of stimulus frequencies and intensities, we observe regions of high-order synchronization, analogous to Arnold Tongues in dynamical systems literature. Significant areas of overlap occur between synchronization regimes, with the bundle intermittently flickering between different winding numbers. We demonstrate how an ensemble of these noisy spontaneous oscillators could be entrained to efficiently detect signals significantly above the characteristic frequencies of the individual cells.
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Introduction
Synchronization and entrainment phenomena have been shown to be ubiquitous in nonlinear oscillators. Already in the 17th century, Huygens demonstrated that two clocks, weakly coupled by a wall, synchronize the swings of their pendula1. Synchronization was investigated in many nonlinear mechanical and electrical devices, from musical instruments to lasers. Mathematical studies have extensively explored the entrainment of a nonlinear oscillator by an imposed oscillatory drive2,3,4. In recent years, a number of biological systems were shown to exhibit nonlinearities in their response to external signals and to display synchronization between constituent oscillators2,5,6,7,8,9,10,11. For example, synchronization was encountered in collective animal behaviors7,8, in cooperation between organs9,12, or even at the level of individual cells10,11. Investigating synchronization and entrainment is of particular interest in explaining the complex nonlinear dynamics of the auditory system. For the sense of hearing, a phase-locked mechanical response to an external sound constitutes a crucial step in auditory detection. Entrainment occurs at many levels in the process of hearing, starting with the response of mechanosensitive hair cells13.
Hair cells of the inner ear are biological sensors that detect displacements induced by air- or ground-borne vibrations and transduce them into electrical signals14,15. They are composed of a cell body and a bundle of 30–50 stereocilia that protrude from the apical surface15,16. Near the thresholds of detection, these cells can be responsive to bundle motions as small as a few Å17. Furthermore, the auditory system is sensitive to a broad range of frequencies, with some species able to detect signals up to 100 kHz and higher18. The biophysical mechanisms behind the extreme sensitivity of detection and the ability to respond to mechanical signals at such high frequencies are still not fully understood.
A compressive nonlinearity arises in the evoked response, measured both in the motility of individual hair bundles and in the response of the whole system - vibrations of the membrane in which the cells are embedded15,17,19. The nonlinear response was demonstrated in a number of different species15,20,21,22, and was shown to be crucial to the sensitivity of detection by the hair cells. Further, it was shown to be an essential nonlinearity23, which is maintained even as incoming signals approach zero amplitude.
Spontaneous oscillations of hair bundles have been observed in several species24,25,26. They result from an internal amplifier that enables the hair cells to sustain their sensitivity under over-damped in vivo conditions15,21,27,28. Two cellular processes are involved in the oscillation process. Gating of the mechanically sensitive ion channels in the stereocilia leads to bi-stability in the position of the bundle. An array of myosin motors has been proposed to be physically connected to the transduction complex, and to climb and slip along the actin filaments that form the core of the stereocilia. This adaptation process continuously adjusts the position of the bundle and, in conjunction with mechanical gating of the transduction channels, leads to spontaneous oscillations26. The characteristic frequency of these spontaneous oscillations defines an internal time scale for the mechanical response of the bundle. Surprisingly, this natural frequency is lower than the frequency range of detection of the corresponding organs. For example, the frog sacculus can detect frequencies up to 120 Hz29, while spontaneous oscillations of individual bundles in vitro typically display characteristic frequencies around 30 Hz26. In the current study, we assess whether an ensemble of slow nonlinear oscillators could efficiently encode frequencies higher than those of their internal clocks. We describe experiments that measure the mechanical response of the sensory epithelium to frequencies covering the full detection range.
One of the common features of nonlinear oscillators is that they can support multi-mode phase-locking4. Systems that are exposed to varying frequencies and amplitudes of stimulus can phase-lock to the imposed signal in a variety of synchronization modes, forming regions of entrainment referred to as Arnold Tongues. We explore whether hair cells of the inner ear exhibit multi-mode synchronization and how this mode-locking could affect higher frequency detection.
A recently developed technique, which takes advantage of nanomagnetism to stimulate hair cells, is used to probe the bundle response at higher frequencies. We observe regimes of synchronization corresponding to different mode-locking ratios. Unlike traditional Arnold Tongues encountered in dynamical systems literature, the measured phase-locking regions show overlapping patterns, with hair bundles intermittently switching between different high-order modes. Numerical simulations suggest potential implications of such behavior for the detection of frequencies higher than the natural frequencies of individual bundles.
Results
Magnetic stimulation
To study the mechanical response of an individual hair bundle, one must apply deflections that mimic those induced by incoming sounds under in vivo conditions. Traditionally, these deflections were imposed either with elastic glass rods, or with fluid jets. To access higher frequencies of stimulation without imposing a mechanical load on the bundles or introducing hydrodynamic artifacts, we used an electromagnetic probe to attract superparamagnetic beads that are conjugated to hair bundles. The beads were composed of iron oxide (Fe3O4) nanoparticles embedded in a 1 μm polymer structure. Their superparamagnetic behavior leads to an instantaneous cooperative action of all the nanoparticles, and hence allows one to impose a substantial force to deflect the bundles. This novel technique was recently demonstrated to enable bundle stimulation at kHz frequencies30, well above the requirements of the current study.
Experiments were performed at room temperature on saccular hair cells of the North American bullfrog (Rana catesbeiana). The sacculi were excised from the inner ear and mounted over a 1 mm hole in a two-compartment experimental chamber, with hair cells exposed to artificial perilymph on the basal side and endolymph on the apical side, as described in previous publications30,31 (see Materials and Methods).
The magnetic beads were conjugated to the hair bundles32 (see Materials and Methods), and an electromagnetic probe, positioned in close proximity (~10–20 μm) to a target hair bundle, was used to impose a square wave stimulus, at various frequencies and force intensities. The nanoparticles being superparamagnetic, they could only be pulled by the electromagnet and not pushed. As a consequence, a rectangular stimulus was the most natural choice. The magnetic force generated by the probe tip on an average bead was calibrated, as shown in Fig. 1a and b. Spontaneous and evoked bundle motility was recorded with a CMOS camera.
Phase-locking of oscillating bundles
Figure 1c,d,e, and f show examples of the innate motility exhibited by a hair bundle and its entrainment by stimulation applied at frequencies that are lower, comparable, or higher than the characteristic frequency f0 of the bundle. For a stimulus frequency fs close to f0, the spontaneous oscillation exhibits progressively stronger 1:1 phase-locking with increasing intensity of the drive. However, a stimulus applied at a frequency that is significantly different from f0 evokes a more complex behavior, with different modes of phase synchronization dominating alternately. The natural frequency f0 was evaluated for each bundle from a ten second recording of spontaneous oscillations.
Synchronization pattern
A nonlinear oscillator that exhibits multi-mode phase locking can form Arnold Tongues. To analyze the patterns of mode-locking between an autonomously oscillating bundle and an imposed drive, we followed mathematical procedures developed for the study of entrainment in noisy and/or chaotic systems. The usual condition for phase-locking in deterministic dynamical systems, |φn,m(t)| < const with φn,m(t) = nφb(t) − mφs(t), does not hold in the presence of strong fluctuations because of the appearance of noise-induced phase slips4,6,12. The parameters n and m are integers, and ϕb,s are phases of the bundle and the stimulus, respectively, defined on the real line. If the phase-locking condition is fulfilled, n stimulus cycles should always fit in m bundle oscillations. In the presence of noise, however, entrainment can only be treated in a statistical fashion, as the appearance of preferred values in the distribution of the cyclic phase difference ψn,m = φn,m mod 2π (see SI. 2). To experimentally determine ψn,m, the phase of the bundle ϕb was computed by performing the Hilbert transform on the bundle response4,33, and the phase of the stimulus ϕs was assumed to be 2πfst.
The n:m vector strengths, defined as Rn,m = 1/N|∑jexp(iψn,m(tj))|, provide quantitative measures of the contribution of the n:m modes of entrainment to the full hair bundle response. Here, N is the number of elements in the sum, and index j corresponds to time. A bundle response that is fully locked in a specific n:m mode would correspond to a constant value of ψn,m in time, and would therefore exhibit a vector strength Rn,m of 1. A random response of the bundle would exhibit a homogeneous distribution of ψn,m between 0 and 2π, resulting in a vector strength Rn,m of 0. We computed these vector strengths for signals applied at various amplitudes and frequencies. Since each stimulus protocol is applied for a limited amount of time (see Materials and Methods), one could obtain a spurious Rn,m, even for an infinitely week stimulus applied at a frequency close to that of the spontaneous oscillation. Over the finite duration of the recording, the stochastic bundle phase diffusion is not sufficient to destroy the phase coherence between the spontaneous oscillation and a fictive stimulus. We evaluated this spurious component by applying the same analysis to bundle motility measured without an applied stimulus, and subtracted the result from Rn,m: . Consequently, ΔRn,m reflects only the effect of the stimulus on the vector strength of the hair cell’s response.
The magnetic stimulus was applied so as to either pull the bundle toward (Fig. 2a) or away (Fig. 2f) from the tallest row of stereocilia. Figure 2c and h present the obtained ΔRn,m. A Rayleigh test was applied to ΔRn,m, and the plots display only the values that were found to be statistically significant. As can be seen from the figures, entrainment was observed for various orders of mode-locking, including fractional locking. High-order regimes of synchronization were consistently seen in the 12 cells studied.
Overlaid synchronization regions in Fig. 2b and g show significant overlaps, where the bundle oscillation could mode-lock to the imposed signal with different ratios of frequencies. Apart from the 1:1 mode, each ΔRn,m remained lower than ~0.3, as multiple modes contributed jointly to the bundle response. Different synchronization modes contributed alternately to the bundle response. The respective n:m vector strength values indicate the contribution of each mode to the global bundle response, integrated over time during the full stimulus train. When the amplitude of stimulation was increased, 1:1 mode-locking was favored (Fig. 2d and e). Records obtained by pulling bundles toward (Fig. 2a–c) versus away (Fig. 2f–h) from the tallest row of stereocilia exhibited strikingly similar characteristics.
Intermittent mode-locking
As shown in Fig. 2, there exist regimes of stimulation where the bundle can phase lock in different ratios to the applied frequency. We focused next on the dynamics of entrainment in these regimes of overlap. Each bundle oscillation, defined as the excursion between the opening (positive excursion) and subsequent closing (negative excursion) of the channels, was detected with software developed in Matlab34 (Fig. 3a). The local winding number w is defined to be the ratio of the period of an oscillation to the period of the stimulus. A sequence of 15 to 70 winding numbers was obtained for every stimulus condition.
Figure 3b plots the dependence of the winding number on the applied frequency, for selected stimulus amplitudes. No phase locking was detected at weak signals. The dispersion observed in the measured w originated from the innate width of the spectral density of spontaneous oscillations. At intermediate signals, bands appeared in the plots, indicating different modes of phase-locking. The overlap between the bands indicates that the system can phase lock to the applied signal at different ratios to the stimulus frequency. At higher amplitudes of stimulation, the bundle favored low winding numbers, and the bands sharpened.
To further characterize the overlap between bands of synchronization, we plotted the local winding number as a function of time, for different frequencies of stimulation (Fig. 3d). An intermittent flicker between different winding numbers was observed.
Ensemble response to a broad range of frequencies
Due to the flicker between different n:m modes, prominent over a wide range of amplitudes, any individual hair bundle cannot unambiguously encode stimulus frequencies higher than f0. We next assessed how an ensemble of such bundles would respond to the same stimulus. Assuming the hair cell to be an ergodic system, we separated each record of the bundle response into sequential oscillations. The individual oscillations were superposed, preserving their phase relative to the stimulus (Fig. 3c). Each bundle oscillation occurred at a number of different modes of phase-locking to the signal. However, the oscillation of the full ensemble reflected the periodicity of the stimulus. Even at higher frequencies, where 1:1 mode-locking was completely lost, the ensemble successfully encoded the stimulus frequency.
Numerical simulations of multi-mode locking
To gain insight into the dynamical response of a single bundle as well as the behavior of an ensemble of identical bundles, we performed numerical simulations based on the models of active bundle motility developed in prior studies15,26,35. Following the description of stochastic bundle dynamics by Nadrowski et al.35, we modeled a single bundle with two variables that describe the deflection X(t) of the bundle and an intrinsic molecular motor displacement Xa(t). In dimensionless form, the model reduces to:
with x(τ) = X(t)/δ, xa(τ) = Xa(t)/δ, τ = t/τ0, δ a characteristic length of the system, τ0 a characteristic time of the system, f(τ) the dimensionless stimulus, ξ(τ) and ξa(τ) Gaussian white noise terms with standard deviation σ. The parameter values for ai were adopted from Nadrowski et al.35, and defined in Materials and Methods; values were chosen that poise the system in the regime of limit cycle oscillation35,36,37. The natural frequency of a noiseless bundle was 6.5 ± 0.5 Hz. The bundle response was simulated under different stimulus conditions and was analyzed with the same procedures applied to the experimental data. The numerical model exhibited the same characteristic behavior for the local winding number (Fig. 4b), showing stochastic flicker between different modes. Further, simulations yielded high-order synchronization regimes with features similar to the experimental results (Fig. 4c). Using the model, we verified that a sinusoidal stimulus evokes the same characteristic response as a square wave stimulus (data not shown), indicating that our observations were not affected by the specific waveform of the periodic stimulus.
Numerical simulations allowed us to examine the role played by noise in the complex response of both a single bundle and an ensemble of bundles. In the case of a single bundle, as the noise intensity increased, we observed an evolution from sharp, non-overlapping Arnold Tongues with high vector strength values to broad, overlapping regions of synchronization, showing lower vector strength values (see SI. 3b–d). The model thus indicates that the coexistence of multiple synchronization modes is dependent upon the presence of a stochastic process.
Focusing on the response of an ensemble of bundles, we defined a Group Vector Strength VS that quantifies the ability of the ensemble to collectively encode the stimulus frequency (see SI. 4). The initial conditions for hair bundles within the group were randomized in the simulation. Increasing the noise intensity had a striking effect on VS. At low stimulus frequencies, VS decreased monotonically with noise. However, at high stimulus frequencies, VS showed a maximum at a non-zero noise intensity (Fig. 4d). The presence of noise therefore helps the ensemble to encode high frequencies, analogous to stochastic resonance predicted for individual hair bundles38. Extracting the intensity of physiological noise from our data, and comparing it to results of the simulations, we found that the innate biological noise is in the range that offers a compromise between the enhancement of detection of high frequencies and maintaining the detection of low frequencies (see SI. 5). Our estimates of the biological noise intensity (0.6 < σ < 2) are consistent with the values used in prior literature, converted into dimensionless form35,39.
Discussion
Nonlinearity of the auditory system has been extensively demonstrated experimentally and modeled using dynamic systems theory23,34,35,36,40,41,42. Variation of internal control parameters poises hair cells near different bifurcations, leading to different phase-locking characteristics13,36,38. Hair bundle exhibits a regime of spontaneous limit cycle oscillation15, the frequency of which reveals an internal time scale defined by the active processes within the bundle. This time scale was seen to be slower than the response needed to explain the full range of frequency detection. We hence explored how a relatively slow nonlinear oscillator responds to frequencies that are high with respect to its characteristic frequency.
A common feature of nonlinear oscillators is that they can exhibit high-order synchronization4. Our results indicate that higher-order mode-locking can be robustly observed in hair cells of the inner ear. This behavior was found to form broad regions of entrainment, with significant overlaps, leading to regimes of multiple possible winding numbers. As a result, rather than displaying the traditional devil’s staircase structure4, the dependence of the winding number on the stimulus frequency showed overlapping plateaus, with intermittent flicker between them. Simulations indicated that this flicker is induced by noise in the response of individual hair bundles. How might such a stochastic system reliably detect higher frequencies of stimulation?
We propose that high frequencies are encoded in the collective response of an ensemble of bundles. Detection requires both the nonlinearity and the stochasticity of the system: the nonlinear response of an individual hair bundle enables it to phase-lock to a signal in different n:m modes, and the stochasticity enhances detection of high frequencies. At the level of a single hair bundle, the presence of noise increases the probability of transitions between channel-open and channel-closed states38. For strong stimuli, this effect favors high frequency synchronization (see SI. 5a). If the stimulus is weak, however, the noise destroys the phase information, and synchronization is lost. The major impact of noise arises from its effect on signal detection by an ensemble of hair cells. We showed that the response of an ensemble of noisy bundles, each flickering randomly between different n:m modes, enables a more efficient mapping of the stimulus periodicity than the response of an ensemble of deterministic bundles, having random initial conditions. This effect is preserved even under weak stimulus (see SI. 5b and c).
The model proposed here produces very weak frequency selectivity, as the oscillators phase-lock to a wide range of signals, at various winding numbers, thus producing a very sensitive broadband detector. The resulting behavior is consistent with the properties of the amphibian sacculus29, and other low-frequency vestibular and auditory systems, which display high sensitivity but broad tuning. Further, the notion that detection proceeds via an ensemble of hair bundles is consistent with the highly convergent patterns of innervation in the sacculus29. Signal detection beyond the hair bundle is outside the scope of the current work; however, experimental evidence supports phase-locking of stochastic spikes in the auditory neurons43,44. An analogous model has also been proposed for the response of an ensemble of neurons, as a means of encoding frequencies than exceed the firing rate of any of the individual neurons45,46. We demonstrate here the crucial role of the noisy flicker between different modes in achieving this effect.
A hair cell of the inner ear constitutes an example of a non-equilibrium system, whose nonlinear response is crucial for its biological purpose: extremely sensitive detection of sound. We observe high-order mode-locking between actively oscillating hair bundles and an imposed signal, with intermittent switching between different synchronization modes, originating from the combination of intrinsic noise and nonlinearity. We hypothesize that a collection of hair cells could take advantage of this effect to efficiently detect high frequencies of stimulation.
Materials and Methods
Biological preparation
The sacculi were excised from the inner ear of the North American bullfrog (Rana catesbeiana) and mounted over a 1 mm hole in a two-compartment experimental chamber, with hair cells exposed to artificial perilymph (110 mM Na+, 2 mM K+, 1.5 mM Ca2+, 118 mM Cl−, 3 mM D-glucose, 1 mM sodium pyruvate, 1 mM creatine, and 5 mM HEPES) on the basal side and endolymph (117.5 mM K+, 2 mM Na+, 0.25 mM Ca2+, 118 mM Cl−, 3 mM D-glucose, and 5 mM HEPES) on the apical side. The solutions were made to mimic the ionic conditions in the sacculus of an intact animal. The solutions were oxygenated for ~15 min prior to use. The overlying otolithic membrane was removed from the epithelium with an eyelash tool, following an 8 min enzymatic dissociation with 15 μg.mL−1 collagenase IV (Sigma-Aldrich), dissolved in artificial endolymph with 4 mM Ca2+. Spontaneous oscillation of hair bundles was typically observed for several hours after dissection. All animal-handling protocols were approved by the UCLA Chancellor’s Animal Research Committee (Protocol Number ARC 2006–043–13C), in accordance with federal and state regulations.
Conjugation of magnetic particles to the hair bundle
Carboxylate-modified Sera-Mag Speedbeads (Fisher Scientific) were conjugated to the hair bundles via letin (Concanavalin A, conA) - glycoprotein interaction. After diluting 20 μL of the bead solution (50 mg/mL−1) in 1 mL of 10 mM phosphate buffer (pH 7.4), the beads were isolated by 2 min centrifugation at 1500 rcf and dispersed in 1 mL of phosphate buffer. EDC (383 μg) and sulfo-NHS (800 μg) were introduced into the solution to activate the surface of the magnetic beads, and dissolved for 15 min with gentle shaking. The beads were then isolated by centrifugation at 1500 rcf for 2 min and dispersed in 1 mL of phosphate buffer. 1.8 mg of conA type VI was dissolved in the solution and allowed to react with the beads for an hour under gentle shaking. When the reaction was finished, the beads were isolated by centrifugation at 1500 rcf for 2 min and dispersed in 1 mL of phosphate buffer; the rinse was repeated three times. After centrifugation of 100 μL of the resulting bead solution the magnetic beads were washed in 200 μL of endolymph and then dispersed in 100 μL of endolymph. One drop (~50 μL) of this bead solution was placed on top of the hair bundle preparation for 30 min. During the 30 min incubation, the two-compartment chamber was flipped upside down to avoid gravitational sedimentation of the beads on the surface of the sacculus.
Detection of hair bundle motility
Experiments were conducted under an upright optical microscope (Olympus B51X) with a 60x water-immersion objective (LUMPLFLN60X, 1.0 NA) and illuminated with an EXFO X-cite 120 W light source. Images were further magnified by a 2x relay lens. They were recorded with a Hamamatsu Orca-Flash4.0 Scientific CMOS camera at 1000 frames per second. The camera was triggered by an analog output module from National Instruments (NI 9263), such that the beginning of the record coincided with the onset of the magnetic stimulus train. The observed bundle was oriented to oscillate along the horizontal axis. Its motion was tracked with software written in Matlab (The MathWorks), which calculates the center of mass of the hair bundle. Time-dependent traces of the movement were then obtained by plotting the center of mass for each frame of the recording. The noise levels in these recordings were estimated to be ~3–5 nm.
Electromagnetic probe
The electromagnetic probe was constructed following previously described methods30,47. A copper wire coil, 70 Ω in resistance, was wound 1000 times around a permalloy magnetic probe tip (DSF System, Korea). A plastic capillary tube was used to create a water circulation jacket around the coil, to prevent overheating of the electromagnet. A permalloy 75 rod (Ni 75%, Fe 25%, 0.8 mm diameter, 25 mm length) was used as the electromagnet core material. The tip of the probe was 10 μm wide. Figure 1a illustrates the configuration of the experiment.
Magnetic stimulation of a hair bundle
The voltage driving the electromagnet coil was generated by an analog output module from National Instrument (NI 9263), connected to an amplifier (PZ-150M, Burleigh), and the current in the coil was monitored by an analog input module from National Instrument (NI 9215). The waveform for the applied voltage was generated using LabVIEW software (National Instrument). The electromagnetic probe was mounted on a motorized micromanipulator (Siskiyou Inc.) and positioned close to the target hair bundle, which had been conjugated with 1–3 magnetic beads. The probe was placed either on the side of the tallest or the smallest row of stereocilia. The electromagnetic probe stimulated the bundle with square waves, sent at 66 frequencies ranging from 5 to 200 Hz in 3 Hz increments, and 14 intensities. The intensity range was dependent on the relative position of the bundle and the probe tip. The maximum force intensity (i.e. with the probe at magnetic saturation) was determined as describe above. The other intensities were calibrated by applying the complete experimental stimulus protocol to an elastic glass rod with magnetic beads attached. The deflection of the rod yielded the ratios between the applied force intensities. For each stimulus intensity, the frequencies were presented in a random order. The different intensities were also randomized to avoid any artifacts due to degradation of the bundle. Twenty stimulus cycles were presented for frequencies below 20 Hz, and one second of stimulation was applied for higher frequencies. After conclusion of each experiment, the bundles still exhibited spontaneous oscillation.
Parameters used in the numerical model
We applied the model developed by Nadrowski et al.35 in dimensionless form
The parameters in the dimensionless form are related to those in ref. 35: , , , a3 = A ≈ 2.5 × 107, , , x(τ) = X(t)/δ, xa(τ) = Xa(t)/δ, τ = t/τ0, τ0 = λ/Kgs ≈ 3.7 × 10−3, f(τ) = Fext(t)/δKgs, ξ(τ) = η(t)/δKgs, ξa(τ) = ηa(t)/δKgs.
We applied the model in the limit where the calcium concentration relaxes instantaneously, and with C0 = 0. We adopted the parameter values chosen by Nadrowski et al.: γ = 0.14, λ = 2.8 μN.s.m−1, λa = 10 μN.s.m−1, Kgs = 750 μN.m−1, Ksp = 600 μN.m−1, d = 8.7 nm, Na = 3000, S = 0.65, fmax = 352 pN, N = 50, T = 25 °C, ΔG = 10 kBT.
All the simulations and data analysis were performed using Wolfram Mathematica 10.
Additional Information
How to cite this article: Levy, M. et al. High-order synchronization of hair cell bundles. Sci. Rep. 6, 39116; doi: 10.1038/srep39116 (2016).
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Acknowledgements
We thank Dr. Sebastiaan Meenderink and Dr. Yuttana Roongthumskul for helpful discussions and feedback. This research was supported by AFOSR grant FA9550-12-1-0447 to D.B. and J.C., NIH grant R21 DC015035 to D.B., Institute for Basic Science (IBS-R026-D1) to J.C., and the Korea Healthcare Technology R&D Project (HI08C2149) to J.C.
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D.B. designed the study. J.C. supervised the chemical and magnetic work. D.B., M.L. and J.K. designed the setup. M.L. and A.M. performed the experiments on hair cells. M.L. and J.-H.L. performed the SQUID experiments. J.-H.L. and J.K. developed the protocol to bind the magnetic beads to the hair bundles. M.L. and A.M. analyzed the data. M.L. performed the numerical simulations. D.B. and M.L. wrote the manuscript.
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Levy, M., Molzon, A., Lee, JH. et al. High-order synchronization of hair cell bundles. Sci Rep 6, 39116 (2016). https://doi.org/10.1038/srep39116
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DOI: https://doi.org/10.1038/srep39116
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