Abstract
Mitochondria play critical roles in meeting cellular energy demand, in cell death, and in reactive oxygen species (ROS) and stress signaling. Most Caenorhabditis elegans loss-of-function (lf) mutants in nuclear-encoded components of the respiratory chain are non-viable, emphasizing the importance of respiratory function. Chromophore-Assisted Light Inactivation (CALI) using genetically-encoded photosensitizers provides an opportunity to determine how individual respiratory chain components contribute to physiology following acute lf. As proof-of-concept, we expressed the ‘singlet oxygen generator’ miniSOG as a fusion with the SDHC subunit of respiratory complex II, encoded by mev-1 in C. elegans, using Mos1-mediated Single Copy Insertion. The resulting mev-1::miniSOG transgene complemented mev-1 mutant phenotypes in kn1 missense and tm1081(lf) deletion mutants. Complex II activity was inactivated by blue light in mitochondria from strains expressing active miniSOG fusions, but not those from inactive fusions. Moreover, light-inducible phenotypes in vivo demonstrated that complex II activity is important under conditions of high energy demand, and that specific cell types are uniquely susceptible to loss of complex II. In conclusion, miniSOG-mediated CALI is a novel genetic platform for acute inactivation of respiratory chain components. Spatio-temporally controlled ROS generation will expand our understanding of how the respiratory chain and mitochondrial ROS influence whole organism physiology.
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Introduction
Optogenetics uses light-sensitive proteins to induce a cellular response. Proteins such as channelrhodopsin and halorhodopsin conduct ions in response to light and have been used to control neuronal activity1. Another class of optogenetic tools is a group of proteins that generate reactive oxygen species (ROS) when illuminated2. These ROS-generating proteins, such as KillerRed3 and miniSOG4, are widely employed for their phototoxic effect. MiniSOG is a small flavoprotein derived from the LOV domain of Arabidopsis phototropin 24. There are two variants of miniSOG, an active version that is able to produce ROS in response to light and an inactive variant that has a cysteine (Cys426) that prevents ROS generation4. MiniSOG has been used as a genetically-encoded photosensitizer for mutagenesis5, correlated light and electron microscopy4,6, cell ablation7,8 and inhibition of the synaptic proteins9. In general, expression levels and protein targeting greatly impact the function of genetic photosensitizers. For example, miniSOG’s ability to kill cells in response to light is highly dependent upon where the protein is targeted7.
Chromophore-Assisted Light Inactivation (CALI) is the selective inactivation of a target protein using a photosensitizer. Upon illumination the photosensitizer generates ROS, oxidizing susceptible residues and impairing protein function. The proximity of the photosensitizer to the target protein, its expression level, and illumination protocol contribute to the selectivity of CALI. Genetically-encoded photosensitizers such as miniSOG have the potential to allow for spatial and temporal control over protein inactivation by direct translational fusion with their coding regions. As with many conditional deletion approaches, CALI enables proteins whose expression is required for development or whose loss is lethal to be studied in the context of a live animal. An additional benefit is that CALI-mediated loss-of-function (lf) is acute; whereas conditional gene ablation or RNA interference’s effects depend upon turnover of existing gene products. The disadvantages are that lf can be incomplete, only mature proteins and not the gene or RNA are targeted, and light itself may have off-target effects. With these caveats in mind, we have developed a CALI approach to investigate the role of a mitochondrial respiratory chain component whose deletion is lethal.
Mitochondria generate the bulk of cellular energy through oxidative phosphorylation, whereby complexes of the electron transport chain generate a gradient that is used to produce ATP. Complex II (succinate:ubiquinone oxidoreductase) occupies a unique position within the mitochondrial metabolism machinery, being both a component of the electron transport chain and the Krebs cycle10. Complex II is comprised of four nuclear encoded subunits: SDHA-1, SDHB-1, SDHC-1 and SDHD-110. SDHC-1, also known as MEV-1, is an integral membrane protein that anchors complex II to the inner membrane and helps coordinate the transfer of electrons onto coenzyme Q10. A point mutation in the C. elegans gene coding of SDHC-1, termed mev-1, increases ROS production and decreases lifespan and brood size11, while mev-1(lf) is lethal. These phenotypes have been recapitulated in an orthologous mouse model, suggesting evolutionary conservation12,13. Complex II dysfunction and ROS overproduction are implicated in Huntington’s disease14, cancer15, Charcot-Marie-Tooth disease type 2A16 and ischemic sensitivity17,18.
Here, we describe a C. elegans model where miniSOG has been fused to the complex II/MEV-1 subunit and integrated into the genome using single-copy insertion. The fusion transgene complements mev-1 mutants, and CALI reduces complex II activity and succinate-driven respiration. Our results suggest that complex II is important for the organism’s responses to metabolic stress and for normal reproductive brood size, which may reflect metabolic potential. Our results also suggest that spermatozoa are selectively susceptible to reductions in complex II activity.
Results
Physiologic expression of miniSOG-tagged complex II subunit complements a mutant phenotype
SDHC-1, coded for by mev-1 on chromosome III, is one of four nuclear-encoded complex II subunits. Mos1-mediated Single Copy Insertion (mosSCI) was used to express a mev-1::miniSOG fusion (including the mev-1 promoter, genomic coding region and 3′ UTR) from the ttTi5605 Mos site in chromosome II, in trans to the native locus (Fig. 1). An inactive miniSOG variant4,8 was also expressed as a control. The integrated transgenes rnySi29, [pTFA12(Pmev-1::mev-1::miniSOG(inactive), unc-119 (C. briggsae)) into ttTi5605 ChrII Mos site], and rnySi19, [pTFA11(Pmev-1::mev-1::miniSOG(active), unc-119 (C. briggsae)) into ttTi5605 ChrII Mos site] are hereafter referred to this as “inactive” and “active”, respectively. The virtue of using MosSCI is that the transgene fusion, being single copy, is expressed close to endogenous levels. We hypothesized that the MosSCI approach would allow us to avoid overexpression artifacts, including stoichiometric disequilibrium between subunits, and would limit the effects of miniSOG-generated ROS to disrupting complex II function. In fact, both the active and inactive fusions were well-tolerated, and neither fusion impaired development or reproduction in a wild-type mev-1 background (Fig. 2A), suggesting the absence of a dominant negative effect.
To test whether the mev-1::miniSOG fusions were active, we assessed their ability to complement a mev-1 mutant. The mev-1(kn1) allele contains a point mutation that results in an increased complex II ROS production, decreased brood size and increased sensitivity to paraquat19. We crossed the miniSOG fusions into the mutant background and found that both mev-1::miniSOG(active) and mev-1::miniSOG(inactive) variants rescued the kn1 paraquat sensitivity (Fig. 2A). Moreover, kn1 brood size was also restored by both fusions, albeit below wild-type levels (Fig. 2B and Supplementary Figure S1). Hence, the active and inactive miniSOG variants were always assessed in parallel to control for light-independent effects.
C. elegans depend on mitochondrial function for development. We hypothesized that CALI would selectively influence development in kn1; active worms, but not in kn1; inactive worms. Surprisingly, blue light (470 ± 20 nm, 10.2 mW/mm2) caused modest but significant dose-dependent reduction in L1 survival in all strains, including the wild-type control (Fig. 3A, adjusted P < 0.05, 2-way ANOVA, Tukey), but there were no differences between the wild-type (+) and miniSOG expressing strains. The kn1 mutant on its own was particularly sensitive to blue light and 5′ application resulted in ~60% reduction in survival, which was significantly different from all other strains. Both the inactive and active miniSOG variants complemented this kn1 phenotype (Fig. 3A). However, as observed in the wild-type background, there was no difference between the variants with light exposure. This result highlights the potential toxicity of blue light, which has been observed particularly with respect to mitochondrial function previously20, and suggests that the kn1 mutant sensitizes worms to its toxic effects.
The observation that CALI did not phenocopy the kn1 mutant suggested several possibilities. First, photo-inactivation of the fusion construct may be incomplete. As per heteroplasmic load tolerance with respect to mitochondrial DNA mutants21, it is possible that partial loss of the rescue fusion nevertheless provided enough “wild-type” protein to suppress the kn1 mutant phenotype.
Moreover, larval development may not be particularly susceptible to acute reductions in complex II activity. However, we noted that although there was not a significant difference between them, the kn1; active worms trended towards reduced survival in response to light compared to the kn1; inactive worms (Fig. 3A). Given these caveats, we reasoned that a CALI phenotype might be revealed by subjecting the worms to an additional stress whose effects are known to be exacerbated by the kn1 mutant. The mev-1(kn1) allele was originally isolated in a genetic screen for mutants that were susceptible to methyl viologen, or paraquat19. Consistent with this reasoning, in the presence of paraquat, CALI of kn1; active reduced survival to a point indistinguishable from the kn1 mutant alone (Fig. 3B). However, CALI also reduced survival in kn1; inactive, though not to a similar extent (Fig. 3B). With the background effect of both the fusion transgenes and blue light toxicity, as well as the possibly confounding gain-of-function effects of kn1 itself, we decided to simplify our model by expressing the mev-1::miniSOG fusions in a mev-1(lf) background.
As expected, both the mev-1::miniSOG active and inactive fusions complemented lethality in a mev-1(tm1081) deletion mutant (Fig. 4). To confirm this result, RNAi against miniSOG was used to suppress complementation. The first generation on RNAi plates exhibited increased dauer formation (Fig. 4E; tm1081; inactive, 76 ± 31% increase over scrambled; tm1081; active, 51 ± 12% increase over scrambled), a C. elegans developmental phenotype that occurs in response to harsh environmental conditions such as population density, food supply and temperature22. By the second generation, miniSOG RNAi phenocopied the tm1081 mutant and caused overt death. No effect was seen in the wild-type (+) strain. Interestingly, mitochondrial dysfunction can be interpreted as a xenobiotic or pathogen attack23, and we noted that the tm1081; inactive and tm1081; active strains both avoided the miniSOG expressing RNAi bacterial lawn.
All of the rescued mutants grew to reproductive adulthood in roughly the same period as wild-type worms. However, both the tm1081; inactive and tm1081; active worms had a delayed egg laying period and decreased brood size (Fig. 4A,B). Of the eggs that were laid, 81 ± 2% (tm1081; inactive) and 81 ± 3% (tm1081; active) hatched. Moreover, we observed an increased propensity to undergo dauer arrest in both the tm1081; active and tm1081; inactive worms despite being uncrowded and well-fed (Fig. 4C). Larva can remain in dauer for up to four months24; however, the dauer arrest was transient (Supplementary Figure S2) with all the worms exiting dauer by 11 days. Hence, consistent with our previous observations using the mev-1(kn1) allele (Fig. 2), the complemented worms exhibited mild abnormalities consistent with incomplete rescue.
Chromophore-assisted light inactivation (CALI) of complex II activity
Directly assessing CALI of complex II required that we account for the light-independent effects of the mev-1::miniSOG transgenes as well as the transgene-independent basal effects of blue light itself. Hence, the remaining data has been normalized first to the illuminated wild-type background to account for blue light and second to the unilluminated tm1081; inactive strain to account for the transgene. A titratable light-dependent inhibition of complex II activity was observed in mitochondria isolated from tm1081; active but not tm1081; inactive or wild-type worms (Fig. 5). This inhibition occurred in both an isolated complex II activity (Fig. 5A) and in succinate-driven respiration (Fig. 5B). Despite inhibition failing to reach 100%, exposure to blue light beyond 5 min resulted in non-specific effects that overshadowed our ability to assess acute complex II inactivation by CALI.
The specificity of complex II inactivation was investigated by measuring the activity of neighboring proteins, including complex I (NADH:ubiquinone oxidoreductase), complex IV (cytochrome c oxidase) and citrate synthase (Fig. 5B). Following 5 min blue light exposure, there were no differences between the tm1081; inactive and tm1081; active strains. We conclude that the extent of miniSOG-mediated CALI was restricted to complex II, as would be expected given its high reactivity and limited radius of action.
Since the loss of mev-1 is lethal we hypothesized that CALI in live worms would, like miniSOG RNAi, phenocopy the tm1081 mutant and cause them to die. However, we observed no lethality in L1 or adult C. elegans subject to acute light exposure (Supplementary Figure S3). De novo synthesis of mev-1::miniSOG or mitogenesis may overcome lost complex II activity, hence suppressing a more overt phenotype. We tested this hypothesis by illuminating L1 larva (a non-lethal insult) and growing them on miniSOG RNAi plates (non-lethal in the first generation) to prevent reconstitution of lost MEV-1 through de novo synthesis. We found no significant difference between the groups (Supplemental Figure S4). However, it is possible that the relevant mev-1 target is in neurons, which are refractory to RNAi. Therefore, we also investigated the cumulative effect of consecutive illumination in adult C. elegans. Three pulses of light provided at 2 hour intervals elicited similarly unremarkable effects as a single pulse (Supplemental Figure S5). However, three pulse provided at 24 hour intervals resulted in a subtle developmental phenotype, with fewer L1 progressing to adulthood (Supplemental Figure S6). This latter result supports the idea that mitogenesis may over time help to suppress the effect of mev-1 CALI.
In addition to mitogenesis, if we postulate that the reduction in complex II activity observed in mitochondria (Fig. 5) reflects activity in the intact worm, the complex II activity remaining after CALI may suffice to support viability. It is also possible that other metabolic pathways can compensate for the loss of complex II activity. Interestingly, mimicking a metabolic stress with either paraquat or the proton ionophore FCCP to partially uncouple mitochondria unveiled a cryptic light-dependent effect on survival in the tm1081; active strain (Fig. 6 and Supplementary Figure S7). These data suggest that in the short term complex II is necessary to meet increased metabolic demands.
CALI of complex II results in a decreased brood size
The mev-1(kn1) mutant has a reduced brood size (Fig. 2B), which is complemented only partially by the mev-1::miniSOG transgenes (Fig. 4B), suggesting a role for complex II in the production of offspring. A single worm produces ~300 embryos, and there is intense metabolic demand during the egg-laying period; approximately the entire body mass of the worm is converted to oocytes in a 24 hour period. Embryo production is supported by mitogenesis in both somatic and germ cells late during larval maturation25. We illuminated staged late L4 larva with blue light (Fig. 7A) and scored the number of viable progeny over a ten day period (Supplementary Figure S8). Light caused a dose-dependent decrease in the number of tm1081; active progeny relative to the tm1081; inactive and wild-type controls (Fig. 7B).
Since this effect could be attributed to either the loss of somatic or germ cell complex II activity, we tested if mating could suppress the light-induced loss of brood size. The hermaphrodite worm is sperm-limited. Mating can increase the number of progeny, but more importantly in this case, if the reduction in brood size was caused by defective sperm mitochondria, we hypothesized that cross fertilization would suppress this phenotype.
Males carrying an integrated GFP reporter (Pdat-1::GFP) were used to distinguish cross-fertilized progeny from self-fertilization (Fig. 8A). Mating resulted in a significant increase in the brood size of wild-type worms (mated wild-type, 493 ± 78 total progeny vs unmated wild-type unmated 282 ± 11 total progeny as shown in Fig. 2A, adjusted P < 0.01, 2-way ANOVA with Tukey multiple comparisons test). However, there was no significant increase in either the tm1081; inactive or tm1081; active total brood sizes with mating (Fig. 8B, unmated data in Fig. 4B, adjusted P = 0.70 and 0.76, respectively 2-way ANOVA with Tukey multiple comparisons test), suggesting that the number of oocytes that can be successfully fertilized is limited in these strains. As oogenesis itself is an energy demanding process, we hypothesize that this is due to an underlying light-independent metabolic defect in the transgenic strains, possibly reflecting incomplete complementation of the null mutant.
While both male and hermaphrodites have sperm, male-derived spermatozoa are larger and outcompete hermaphrodite-derived spermatozoa26. In this regard, the wild-type hermaphrodites had an almost full suppression of self-fertilization resulting in 91 ± 4% cross progeny. For unknown reasons, this value was reduced to 71 ± 9% and 73 ± 3% in the tm1081; inactive and tm1081; active hermaphrodites, respectively. Due to this disparity, the data were not normalized to wild-type, and hence the miniSOG-independent effects of blue light are not accounted for. However, even so, it is clear that light selectively decreased the number of self-fertilized progeny in the tm1081; active strain but not the tm1081; inactive strain (Fig. 8C,D). More importantly, this effect was specific to self-fertilized progeny, as cross-progeny brood size exhibited no light-dependence in the tm1081; active strain (Fig. 8B). These results suggest that CALI of spermatozoa complex II and loss of mitochondrial function in these specific cells contributed to the miniSOG-mediated effects of blue light on brood size.
Discussion
miniSOG is a versatile optogenetic tool that has been used for electron microscopy4,6, cell ablation7,8, mutagenesis5 and CALI of synaptic proteins9. The precise mechanism of miniSOG photosenstization remains unclear, as reports on the type of ROS and quantum yield vary2,4,7,27,28. Moreover, the prevailing photosensitization mechanism in vitro may differ under in vivo conditions and the mechanism could be the result of a combination of photosensitization processes. MiniSOG was first described as a singlet oxygen generator4. This type of ROS is highly reactive and does not directly convert with endogenous ROS species such as superoxide or hydrogen peroxide. To develop a system for the selective inactivation of complex II of the mitochondrial respiratory chain, we used miniSOG for CALI of the SDHC subunit, coded for in worms by mev-1. A simple transgene array containing multiple copies of the mev-1::miniSOG fusions was deemed likely to generate overexpression artifacts. Hence, we incorporated our transgene into the ttTi5605 loci on chromosome II using MosSCI for single copy expression.
The miniSOG-tagged mev-1 fusions were functional and could complement two independent mev-1 mutant alleles, both the kn1 missense mutant which generates high levels of superoxide, as well as the tm1081 deletion mutant. Although miniSOG is comparatively smaller than other photosensitizers4, the tag may nevertheless impede the organization or function of complex II. This is consistent with the incomplete phenotypic suppression observed in brood size with both the active and inactive miniSOG transgenes, independent of light (Figs 2 and 4). In addition, the C. elegans life cycle has checkpoints that are influenced by environmental conditions such as food availability29. Nutritional scarcity during early development can result in growth arrest or entry into the dauer stage. During the L1 to L2 transition worms shift reliance from the glyoxylate cycle (complex II independent) to aerobic metabolism (complex II dependent)30. Dauer worms do not exhibit this metabolic shift. The appearance of a transient dauer phenotype in the tm1081; inactive and tm1081; active strains may be the result of decreased expression/activity of complex II resulting in a perceived food scarcity. Hence, our ability to normalize for light-independent effects of transgene rescue using the inactive mev-1::miniSOG fusion strain was a critical control.
We also found that blue light exposure itself results in adverse consequences on development and fertility. miniSOG requires a flavin mononucleotide cofactor, and flavins are critical to mitochondrial biology4. We hypothesize that blue light can negatively impact the flavin-rich mitochondria, though the specific consequences on individual components of mitochondrial function are as-of-yet unknown. We note that there is a ~35% reduction in complex I activity in response to illumination in both the tm1081; inactive and tm1081; active strains (Fig. 5B). C. elegans also have neurons that mediate behavioral responses to blue light31. However, it is less likely that these neurons are the physiologic basis of the blue light sensitivity, as the effect is manifest in purified mitochondria as well as the whole organism. Longer periods of illumination in our hands elicited confounding effects, and short bursts of intense blue light may be optimal for CALI approaches. Recently, mutations to miniSOG have rendered a version that in vitro has a larger quantum efficiency of singlet oxygen generation32. This advance may decrease the blue light exposure time as well as the potential for nonspecific effects. In addition, genetically-encoded photosensitizers that respond to longer wavelength light may elicit fewer potential off-target effects3,33.
While genetic ablation of mev-1 is lethal, the acute loss of complex II by CALI is survivable. We acknowledge that CALI-mediated complex II loss-of-function is likely not complete (Fig. 5), while metabolic alternatives may also compensate in the short-term and mitogenesis in the long term. C. elegans have been shown to exhibit remarkable metabolic plasticity and adapt astoundingly to their environment. For example, alternative energy pathways such as the glyoxylate cycle30 may shift metabolism away from the electron transport chain and ultimately decrease complex II dependence30. A strain containing gas-1(fc21) has a reduction-of-function mutation in a complex I subunit that is accompanied by a compensatory upregulation of complex II34,35, suggesting that some level of crosstalk occurs between these two conduits to the electron transport chain. It is also notable that attempts to obtain a gas-1(fc21) double mutant with either tm1081; inactive and tm108; active were unsuccessful. In the long term, respiratory chain damage could induce compensatory responses. Many of the canonical mit mutants in C. elegans exhibit constitutive activation of the mitochondrial unfolded protein response and require this adaptive response to remain viable36. While we do not observe induction of a mitochondrial chaperone hsp-60::GFP reporter following complex II CALI, we cannot rule out an age-dependent compensatory effect through this or other pathways.
Our data in general showed that CALI of complex II rendered worms sensitive to mild metabolic stresses. It is likely that the loss of complex II resulted in worms that are unable to respond to increased metabolic demand. It is unclear, however, whether this applies selectively to complex II or whether the same holds true for other bioenergetic measures. CALI of complex II also selectively impairs spermatozoa, resulting in reduced brood size. Spermatogenesis occurs both in hermaphrodites and males and begins at early L4 larval stage37. During development, hermaphrodites switch from spermatogenesis to oogenesis and are therefore sperm limited. Hence, the introduction of male sperm can increase the number of progeny. During self-fertilization, many spermatozoa are pushed from the spermatheca to the uterus, requiring the spermatozoa to crawl back37. Spermatozoa contain about 50 to 70 mitochondria38, and may be considered translationally silent39. We hypothesize that complex II is important in meeting the energy demand necessary for motility and spermatozoa are unable to cope with the loss of complex II. In fact, many nuclear-encoded respiratory genes exhibit a strong maternal effect loss of function phenotype, and RNAi of these gene products exerts a more potent effect than is observed in null animals born to heterozygous parents40,41. We hypothesize further that because of the limited period over which fertilization and embryonic development occurs, acute inactivation of respiratory function is more likely to have pronounced effects in germ cells, where there is less time to adapt.
Overall, we have demonstrated an approach to selectively inactivate a mitochondrial respiratory complex with light. This model facilitates studying the contribution of individual respiratory chain components whose lf phenotypes have heretofore been limited to RNAi42,43,44. The development of more efficient photosensitizers with different spectral responses may increase the utility of this technique, and perhaps allow us to distinguish lf effects from the effects of ROS itself.
Methods
C. elegans Strains, Alleles and Culturing Techniques
C. elegans were cultivated using standard laboratory practices45. Unless indicated otherwise, all strains were grown at 20 °C on Nematode Growth Media (NGM)-agar plates seeded with the bacterial strain OP50 as a food source. In addition, strains were grown in the dark (though routine propagation utilized transmitted light stereomicroscopes to move worms). N2 (Bristol) was used as the wild-type strain. A complete list of mutants and strains used in this work is available in Supplementary Table S1. Strain abbreviations used throughout the text are summarized in Fig. 1 and the Supplementary Table S1.
The mev-1::miniSOG transgenesis was accomplished through Mos-mediated single-copy insertion46. Briefly, the coding region of miniSOG (courtesy of Drs. Roger Tsien and Yishi Jin, University of California, San Diego) was inserted between the mev-1 promoter/genomic coding region and the translational stop site/3′ UTR to code for a fusion protein where SDHC is tagged with active miniSOG (pTFA9 vector) or inactive miniSOG (pTFA10 vector) at its C-terminus. An SpeI fragment from these vectors was inserted into the MosSCI base vector pCFJ15146 to create pTFA11 and pTFA12, which were injected into the gonads of unc-119 mutant worms and integrated at the ttTi5605 Mos locus on chromosome II using positive and negative selection as described previously46. Following extensive outcrossing, the genomic loci at the insertion sites were PCR amplified and sequenced fully to verify the absence of indels or mutations.
The vector for miniSOG RNAi contains from ~35–280 of the miniSOG coding region, cloned as a PCR-tagged BamHI-XbaI fragment into the base vector pPD129.36 (courtesy of A. Fire).
Conventional mating protocols were used to cross the MosSCI transgenes (chromosome II) with mev-1 mutants (chromosome III). Genotyping was through single worm PCR. The kn1 allele is a point mutation in which a G becomes an A and introduces a genomic BspEI site. mev-1 geno F1 (TTT TTG TGT ACG CAT GAA GGA GAA) and mev-1 geno R1 (AGG GCT GGG GCG GAG TAA GAA) primers were used to PCR amplify a 632 nt fragment. Digestion with BspEI results in a 194nt and a 438nt fragment in the kn1 mutant. For the deletion allele tm1081 a combination of three primers was used, mev-1 geno F1, mev-1 geno F3 (TGG CTG TCC ATT TGG AAG CAC CC) and mev-1 geno R1. The expected product size is 1172 nt and 632 nt from wildtype and 300 nt for the tm1081 deletion. The MosSCI transgene was amplified using OG967 and OG970 primers flanking the ttTi5605 recombination interval, as described46. Repeat genotyping of multiple offspring in later generations was used to confirm homozygosity.
RNA interference (RNAi)
RNAi was performed as previously described47. Briefly, HT115 bacteria were transformed with either an empty vector or vector targeting miniSOG, as above. The bacteria were grown at 37 °C to midlog phase, induced with 1 mM isopropyl-β-D-thiogalactosidase (IPTG) for 1 h, and seeded on NGM plates that also contained 1 mM IPTG. Three days later, L3 larval worms (F0) were placed on the RNAi plates, moved to new plates at 24 h, and their progeny (F1) were screened for phenotypes. The F1 generation was then moved to a fresh plate, and their progeny (F2) were also screened.
Illumination
We employed an Olympus MVX stereomicroscope to expose worms to blue light using X-Cite® (Excelitas Technologies Corp., Waltham, MA) illumination filtered through a GFP filter set (470-nm excitation ± 20-nm; Chroma, Bellows Falls, VT). Worms were restricted by suspending them in a 10 μL drop of M9 buffer. Isolated mitochondria were also illuminated in 10 uL drop at a concentration of 25 mg/mL. Identical acquisition conditions, including optics (2x objective), magnification (4x) and illumination settings on the X-Cite were used to standardize light intensity for all of the strains and conditions tested in this work. This intensity was measured empirically at 10.2 mW/mm2 using a calibrated silicon photodetector connected to an optical power meter (model number 818 P-010-12, Newport Corporation, Irvine, CA).
Mitochondrial Isolation, enzymatic assays and respiration
Mitochondria were isolated from staged young adult C. elegans, as previously described using differential centrifugation in mannitol and sucrose-based media48,49.
Following isolation, mitochondrial respiration was measured using a Clark-type O2 electrode (Hansatech Instruments, UK) as previously described48. Briefly, mitochondria (1 mg/ml protein) were suspended in respiration buffer (120 mM KCl, 25 mM sucrose, 5 mM MgCl2, 5 mM KH2PO4, 1 mM EGTA, 10 mM HEPES, 1 mg/ml fat-free BSA, pH 7.3 at 25 °C). Complex-specific substrates (complex II, 5 mM succinate; complex IV, 2.5 mM/2.5 μM Ascorbate/TMPD) were used to initiate state 2 respiration. State 3 respiration was initiated with 200 μM ADP.
Isolated enzymatic activities of complex I, II and citrate synthase were determined spectrophotometrically using fresh mitochondria that were freeze-thawed as previously described. Complex I was calculated as the rotenone-sensitive rate of NADH-oxidation (ε = 6180 M−1 at 340 nm)50. Complex II activity was determined as the succinate-driven, malonate-sensitive rate of DCPIP-reduction (ε = 21000 M−1 at 600 nm)18. Citrate synthase activity was determined as the rate of DTNB-coenzyme A formation (ε = 13600 M−1 at 412 nm)51.
Viability assays
To assess paraquat sensitivity, synchronized L1 larval worms were illuminated for the indicated time and placed on NGM plates seeded with OP50 and containing paraquat at 0–300 μM in 50 μM increments. Viability was scored after 4 days, with survival defined as the number of animals progressing beyond L4 in that period. For FCCP studies, synchronized L4 were illuminated for the indicated time and cultured on a NGM plate seeded with OP50 containing 1 μM FCCP. Viability was assessed 1 day later. A worm was defined as dead if it failed to move forward or backward in response to a gentle touch to the head.
Brood size
Staged L4 worms were illuminated for the indicated amount of time and moved individually to fresh plate OP50-seeded NGM plates every 24 hours for 10 days. Progeny were counted on each plate 24–48 hours after removing the illuminated individual. For mating brood size assays, staged L4 worms were placed on a mating plate (containing a smaller amount of OP50, but food was not restrictive) with 10 transgenic males. The worms were moved to a new mating plate every 24-hours, and progeny were scored 24–48 hours after that. Cross-fertilized progeny were identified by the presence of GFP in the dopaminergic neurons.
Additional Information
How to cite this article: Wojtovich, A. P. et al. Chromophore-Assisted Light Inactivation of Mitochondrial Electron Transport Chain Complex II in Caenorhabditis elegans. Sci. Rep. 6, 29695; doi: 10.1038/srep29695 (2016).
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Acknowledgements
Several strains were provided by the C. elegans Gene Knockout Consortium, the Japanese National Bioresource Project, and the C. elegans Genetics Center, which is funded by NIH Office of Research Infrastructure Programs (P40 OD010440). We thank Dr. Doug Portman for the UR314 strain and members of the Western New York Worm Group and Dr. Paul Brookes for helpful discussions. This work was funded by grants from the National Institutes of Health to APW (R01 NS092558) and Paul Brookes & KN (R01 GM087483) and from the National Science Foundation to KN (IOS1352836).
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Conceived and designed the experiments: A.P.W. and K.N. Performed the experiments: A.P.W., A.Y.W., T.A.S. and K.N. Analyzed the data: A.P.W., A.Y.W. and K.N. Wrote the paper: A.P.W., K.N. and T.H.F. All authors reviewed the manuscript.
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Wojtovich, A., Wei, A., Sherman, T. et al. Chromophore-Assisted Light Inactivation of Mitochondrial Electron Transport Chain Complex II in Caenorhabditis elegans. Sci Rep 6, 29695 (2016). https://doi.org/10.1038/srep29695
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DOI: https://doi.org/10.1038/srep29695
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