Abstract
Modulation of the A type γ-aminobutyric acid receptors (GABAAR) is one of the major drug targets for neurological and psychological diseases. The natural flavonoid compound luteolin (2-(3,4-Dihydroxyphenyl)- 5,7-dihydroxy-4-chromenone) has been reported to have antidepressant, antinociceptive and anxiolytic-like effects, which possibly involve the mechanisms of modulating GABA signaling. However, as yet detailed studies of the pharmacological effects of luteolin are still lacking, we investigated the effects of luteolin on recombinant and endogenous GABAAR-mediated current responses by electrophysiological approaches. Our results showed that luteolin inhibited GABA-mediated currents and slowed the activation kinetics of recombinant α1β2, α1β2γ2, α5β2 and α5β2γ2 receptors with different degrees of potency and efficacy. The modulatory effect of luteolin was likely dependent on the subunit composition of the receptor complex: the αβ receptors were more sensitive than the αβγ receptors. In hippocampal pyramidal neurons, luteolin significantly reduced the amplitude and slowed the rise time of miniature inhibitory postsynaptic currents (mIPSCs). However, GABAAR-mediated tonic currents were not significantly influenced by luteolin. These data suggested that luteolin has negative modulatory effects on both recombinant and endogenous GABAARs and inhibits phasic rather than tonic inhibition in hippocampus.
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Introduction
Luteolin (PubChem CID: 5280445) is a naturally occurring flavone with four additional hydroxyl groups at C3′, C4′, C5 and C7 on the flavone backbone of 2-phenylchromen-4-one (2-phenyl-1-benzopyran-4-one)1. Luteolin is found in many vegetables and medical herbs, such as Perilla frutescens2,3. The pharmacological effects of luteolin have been widely reported, such as antioxidant, anticarcinogenic and anti-inflammatory activities3,4. Interestingly, recent studies have indicated that luteolin might have psychopharmacological effects in the central nervous system (CNS) at least partially through activation of GABAARs5,6,7,8,9,10. However, detailed studies that examine the pharmacological effects of luteolin on GABAAR functions are still lacking.
GABAARs are the major inhibitory receptors in the CNS. At least 19 subunits of GABAARs exist in the human nervous system, including α1-6, β1-3, γ1-3, θ, π, ε and ρ1-311. The expression of GABAAR subunits is not limited to the nervous system but is also widely found in peripheral non-neuronal organs, including the lung, pancreas, gut etc. In the peripheral systems such as the lung tissue, endogenous GABAARs could either exist as the αβ isoform or incorporate a γ-like subunit12,13. These peripheral GABAARs are involved in various physiological and pathological conditions, including modulation of glucagon release, mucus overproduction and prevention of cell death in pancreas and liver13,14,15,16,17. In the adult CNS, the major isoforms of GABAARs are composed of α, β and γ subunits12,18. Varied compositions of GABAARs are differently distributed in synaptic and extrasynaptic regions of the inhibitory synapses to control balance between neuronal excitation and inhibition for normal brain functions. The high diversity of subunit compositions indicates that the GABAAR subtypes located at different brain regions and different subsynaptic loci are engaged in distinct functions19. For example, in the hippocampus, the α1β2γ2 GABAARs mediate the phasic inhibitory transmission at synapse, whereas the α5β2γ2 GABAARs are located at extrasynaptic sites to mediate tonic inhibition in response to ambient release of GABA12. Previous studies showed that knockout α5 subunit of GABAARs or selective inhibition of α5β2γ2 function improved learning and memory abilities20,21, while enhancement of synaptic GABAAR function has always been an important target of treating epilepsy, anxiety and other psychiatric/neurological diseases12.
Despite accumulating studies suggesting a modulatory effect of luteolin on GABAARs in the CNS7,9, the pharmacological characterization of luteolin still remains unclear. For instance, studies have shown that luteolin has antidepressant and analgesic effects that are considered to be mediated by enhancing GABAAR functions6,8; however, luteolin did not exhibit any pro- or anti-convulsant effects in various animal models of epilepsy22. Furthermore, luteolin has been reported to enhance learning and memory in a neurodegenerative model10. Children with autism spectrum disorders showed improvement in adaptive functioning after receiving dietary supplement of flavonoids including luteolin (100 mg/10 kg weight daily)23, which produced an estimated concentration of ~8.7 μmol/L in plasma according to a study on the relationship between oral intake and plasma concentration of luteolin24. Hence, these previous studies have led us to hypothesize that luteolin might have varied effects on different subtypes of GABAARs to achieve the complex neuropharmacological influences. To elucidate the pharmacological mechanisms underlying these study results, we investigated the sensitivities of α1- and α5-containing GABAARs to different doses of luteolin in HEK cells. Furthermore, the effect of luteolin on α1- and α5- containing GABAAR- mediated phasic and tonic inhibition in hippocampal slices was also studied.
Results
Effects of luteolin on dose-response relationships of GABAARs in HEK cells
Inclusion of α and β subunits is required to create functional GABAAR pentamers and the αβ receptors are already sufficient for insertion into the plasma membrane. Despite low levels of expression, the αβ receptors naturally exist in the CNS and in peripheral systems13,25. The αβ receptors are also useful for molecular pharmacology studies using recombinant expression systems like HEK cells. The αβγ receptors are the most abundant form of GABAARs in the CNS and are sensitive to benzodiazepine potentiation. Among the αβγ compositions, the α1β2γ2 receptors are the most predominant form and are ubiquitously distributed in inhibitory synapses to mediate phasic inhibition, whereas α5β2γ2 receptors are predominantly located at the extrasynaptic region to mediate the tonic inhibition in hippocampus. In the present study, we selected recombinant α1β2, α1β2γ2, α5β2 and α5β2γ2 GABAARs to characterize the pharmacological effects of luteolin.
Whole-cell recordings were performed in HEK293T cells expressed with α1β2, α1β2γ2, α5β2, or α5β2γ2 subunits of GABAARs. Fast-perfusion of GABA (0.1–500 μM) produced inward current responses at a holding membrane potential of −60 mV. Recombinant expression of GABAARs resulted in dose-response curves with EC50 values within a range of 2 to 4 μM (Fig. 1, Table 1). Extracellular application of luteolin (50 μM) inhibited current responses induced by the agonist from medium to saturating doses (1–500 μM GABA) in all tested forms of GABAARs. Although the maximum currents (Imax) of GABAARs were substantially reduced by luteolin, the EC50 was relatively less affected (with 0.8-, 1.7-, 1.6- and 2.1-fold changes by 50 μM luteolin in α1β2, α1β2γ2, α5β2 and α5β2γ2 receptors, respectively, Table 1). These results indicate that luteolin is likely a non-competitive antagonist of GABAARs.
Potency and efficacy of luteolin on different forms of GABAARs
We next examined the potency and efficacy of luteolin on the four forms of GABAARs by testing the dose-response relationship of luteolin-mediated inhibition. 0.1–100 μM of luteolin was included in the perfusion solution and was applied onto the same cell in sequence. By using medium doses of GABA to induce current responses, luteolin strongly inhibited GABA currents at concentrations>=10 μM (Fig. 2), which exhibited a similar pattern to the effects of other flavonoids like apigenin and quercetin on GABAARs26,27. By comparing the IC50 of luteolin on the four types of GABAARs, we found that luteolin inhibited GABA currents with similar potency, with the lowest IC50 in α1β2 (10.8 ± 4.46 μM). In the other forms, the IC50 values of luteolin are 51.4 ± 242.8 μM in α1β2γ2, 25.4 ± 9.0 μM in α5β2 and 27.5 ± 13.8 μM in α5β2γ2. Moreover, the inhibitory efficacy of luteolin was assessed by the extent of reduction in Imax. Our results showed that luteolin had better efficacy on α1β2 and α5β2 receptors (Table 1). Inclusion of the γ2 subunit decreased the efficacy of luteolin, suggesting that the γ2 subunit is not required for forming luteolin binding site for its inhibition.
We also compared the inhibitory efficacy of luteolin on current responses mediated by medium or high doses of GABA. In the α1β2 and α5β2 receptors, both medium- and high-dose GABA-mediated currents were significantly inhibited by 50 μM of luteolin (Fig. 3A,C). In the α1β2γ2 receptors, however, luteolin had an inhibitory effect on 500 μM but not 3 μM GABA-induced currents (Fig. 3B). In the α5β2γ2 receptors, luteolin showed a better efficacy on 2 μM compared with 500 μM GABA-mediated currents (Fig. 3D). Taken together, these data showed that luteolin exhibited varied degrees of potency and efficacy to reduce current responses of different forms of GABAARs.
Luteolin affected activation kinetics of GABAARs
Activation kinetics of GABA currents can influence the time course of the neurotransmitter-evoked responses at the synapses28. In the present experiments, currents were elicited by fast perfusion of GABA at concentrations close to EC50 and the activation kinetics were characterized by the 10–90% activation time of the current after fast perfusion of GABA. Luteolin at high concentrations (100 μM) slowed the activation time in all tested forms of GABAARs (Fig. 4). In the presence of 100 μM luteolin, the activation time of α1β2, α1β2γ2, α5β2 and α5β2γ2 GABAARs after luteolin treatment was increased by 3.5-, 2.4-, 5.8- and 4.1-fold, respectively. Luteolin concentration lower than 10 μM had no significant effects on GABAAR activation time. Here we selected 0.1 and 100 μM as the representative doses of luteolin to show their effects on the activation kinetics of GABA currents. Our data suggested that higher doses of luteolin prolonged activation time of GABAARs (Fig. 4).
Effects of luteolin on phasic currents mediated by GABAARs in hippocampal slices
The hippocampus is the critical locus for learning and memory. The α1β2γ2 GABAARs are located at the postsynaptic site of inhibitory synapses and predominantly mediate the fast inhibitory synaptic currents in hippocampus. To further investigate the pharmacological modulation of luteolin on endogenous α1β2γ2 GABAARs under physiological conditions, we tested the effect of luteolin on phasic inhibition in hippocampal slices. The GABAAR-mediated mIPSCs were pharmacologically isolated by inclusion of the sodium channel blocker TTX (0.5 μM) and the AMPA/kainate receptor blocker CNQX (20 μM). At the end of each experiment, the GABAAR antagonist bicuculline (10 μM) was applied to confirm that the mIPSCs were abolished (Fig. 5A). Here we selected two representative doses (0.1 and 100 μM) to test the effect of luteolin. We showed that 100 μM luteolin significantly decreased the amplitude (87.7 ± 3.4% normalized to baseline) but not the frequency (103.6 ± 6.2%) of mIPSCs (Fig. 5A–D). The rise time of mIPSCs was also significantly slower after high dose of luteolin treatments (108.6 ± 2.0%) (Fig. 5E). In contrast, 0.1 μM luteolin did not show any significance in changing the frequency (93.7 ± 3.1%), amplitude (98.9 ± 0.9%), or the rise time (102.7 ± 2.7%) of mIPSCs (Fig. 5C–E). Together, these data indicated that luteolin exerts negative modulation on phasic inhibitory responses by reducing the amplitude and slowing down the activation time of synaptic currents.
Effects of luteolin on tonic currents mediated by GABAARs in hippocampal slices
The α5β2γ2 GABAARs are located at the extrasynaptic site and mediate tonic inhibition in hippocampus. Our data have shown that, in the recombinant α5β2γ2 GABAARs, luteolin inhibited medium to high doses GABA-mediated responses. Considering that tonic inhibitory currents in the CNS are ascribed to the low concentration of ambient GABA in the extracellular space (from 0.2 to 2.5 μM)29, it still remained to be determined whether luteolin has any modulatory effect on the low-dose GABA-mediated responses in slices. In our experiments, tonic currents were defined as the shift of holding currents after application bicuculline (10 μM). In some experiments, we also included a low dose of GABA in the bath solution (0.5 μM) to unify the ambient GABA concentration. Treatments with 0.1 or 100 μM luteolin did not significantly affect the amplitudes of tonic currents (P > 0.05 for both 0.1 and 100 μM groups) (Fig. 6). These results suggested that luteolin did not influence extrasynaptic GABAAR-mediated tonic inhibitory currents.
Discussion
The pharmacological effect of luteolin on recombinant and endogenous GABAARs
Luteolin has been widely studied for its pharmacological effects, including anti-inflammatory, anti-oxidant and anticarcinogenic activities. Recent studies have suggested that luteolin might enhance the function of GABAARs, thereby producing antihyperalgesic, anxiolytic and antidepressant-like effects in the CNS5,6,8. However, it still remains unclear whether luteolin takes these effects by directly targeting on GABAARs. In the brain, modulation of distinct subunit compositions of GABAARs is associated with different neurological and behavioral outcomes. Enhancements of α1β2γ2 GABAARs that mainly mediate fast synaptic inhibition generally produce sedative effects, while inhibition of α5β2γ2 GABAARs by either pharmacological or transgenic approaches can promote learning and memory. In the present study, we showed that low concentration of luteolin (0.1 μM) did not affect either GABAAR-mediated phasic or tonic currents. In contrast, high concentration of luteolin (100 μM) reduced the amplitude of mIPSCs and prolonged the activation time course, but had no effect on mIPSC frequency. This indicated that the effect of luteolin was likely due to postsynaptic rather than presynaptic modulation. The inhibition of mIPSCs by luteolin was consistent with the observation from HEK cells: high concentration of luteolin suppressed the amplitude and prolonged the activation kinetics in recombinant GABAARs including the α1β2γ2 form. Although we have shown that luteolin reduced current responses that were induced by high dose but not medium dose of GABA in recombinant α1β2γ2 receptors (Fig. 3D), it was still in line with the results from slices since vesicular release of GABA at synapses normally reaches a very high concentration30. Furthermore, our results are consistent with previous studies that luteolin did not affect the threshold of seizure induction by pilocarpine or electrical stimuli in animal models22, suggesting that luteolin cannot produce obvious sedative effect through enhancement of the function of α1β2γ2 GABAARs.
The tonic inhibition in hippocampus was unaffected by luteolin even at concentrations as high as 100 μM. However, the α5β2γ2 GABAAR-mediated responses in HEK cells were significantly reduced. This could be ascribed to the low concentration of ambient GABA in the extracellular space in hippocampal slices, since luteolin showed very weak efficacy to modulate low dose of GABA-mediated responses (at [GABA] < 1 μM) (Fig. 1D). Therefore, our findings indicated that luteolin is a negative modulator for GABAARs and did not have any potentiation effect on phasic or tonic inhibition in hippocampus.
Previous studies of luteolin mostly focused on the in vivo effects. One of the major differences between the in vivo and in vitro environments is the temperature. In our study, we performed in vitro electrophysiological experiments in room temperature (23–25 °C), which was lower than the body temperature (37 °C). Notably, some allosteric modulators, such as zolpidem, can modulate GABAARs in a temperature-dependent manner31. The affinity of zolpidem to GABAARs increased along with the increasing temperature from 16, 26 to 36 °C31. Previous studies showed that luteolin was stable at 37 °C in culture medium for 24 hours32. We thereby predict that luteolin might consistently take effects and show increased inhibition on GABAARs in vivo. Moreover, our data revealed that luteolin showed stronger inhibitory effects on recombinant GABAARs in HEK cells than in the endogenous GABAARs in brain slices. It was possibly due to the lack of synaptic scaffolding proteins in HEK cells and this might affect luteolin-mediated inhibition on GABAARs as suggested by previous studies33.
Luteolin likely targets at non-benzodiazepine binding sites of GABAARs
Benzodiazepines are one of the most potent positive modulators of γ-containing GABAARs and the binding site is located at the α(+)/γ(−) interface. Previous studies have indicated the structural similarity between flavones and benzodiazepine ligands34. Among different types of flavones, the presence of electronegative groups at the C6 and C3′-position are critical determinants for high affinity to the benzodiazepine-binding site35. For instance, hispidulin (4′,5,7-trihydroxy-6-methoxyflavone) bearing a methoxyl group at C6 is a potent benzodiazepine site ligand and potentiates GABAAR-mediated responses in α1β2γ2 form but not in α1β2 form receptors36. The structure of luteolin (5,7,3′,4′-Tetrahydroxyfavone) is similar to apigenin (5,7,4′-Trihydroxyflavone) and quercetin (3,5,7,3′,4′-Pentahydroxyfavone), all of which lack electronegative moieties at C6. Previous studies have demonstrated that luteolin and quercetin have weak affinities for the benzodiazepine site, with Ki values over 100 μM for the [3H]flunitrazepam binding competition35. Apigenin exhibited a higher affinity for central benzodiazepine receptors (with a Ki of 4 μM to compete [3H]flunitrazepam binding)37 and exerted anxiolytic and antidepressant effects in in vivo animal models. However, whether a direct involvement of GABAARs in the CNS is responsible of the effects of apigenin remains questionable. Electrophysiological studies showed that apigenin and quercetin similarly inhibited GABA-induced currents, while the inhibition of apigenin on α1β2γ2 GABAAR-mediated responses were not prevented by the benzodiazepine site antagonist flumazenil26. These studies indicated that, different from the traditional anxiolytic chemical benzodiazepine, the CNS effects of apigenin and quercetin are not likely due to their direct interaction and potentiation of GABAARs. In the present study, we found that luteolin negatively modulated GABAARs that lacked γ subunits. In agreement with previous studies using the [3H]flunitrazepam binding assay, our results indicated that luteolin inhibited GABAARs through non-benzodiazepine site of GABAARs. Such a modulatory effect of luteolin is in resemblance of that of apigenin and quercetin. We did not observe apparent potentiation effects of luteolin on either α1- or α5- containing GABAARs that were reported for hispidulin, likely due to the lack of a hydroxyl group at the C6 position of flavone9. Therefore, although we did not exclude the possible interaction between benzodiazepine sites and luteolin’s metabolites, the direct effect of luteolin on GABAARs did not involve binding to central benzodiazepine receptors. In other words, the CNS effects of luteolin are likely obtained through other pharmacological mechanisms, possibly similar to those of apigenin and quercetin due to their structural similarity.
Different modulation on αβ and αβγ forms of GABAARs indicated possible luteolin-binding sites
During the development of benzodiazepine ligands, researchers have discovered new allosteric modulation sites on GABAARs. Ramerstorfer et al. have revealed a new ligand-binding site at the α(+)/β(−) interface that was independent from the benzodiazepine site at the α(+)/γ(−) interface38. This new binding site was discovered by screening of benzodiazepine site ligand and was determined by CGS 9895 that could potentiate GABAARs in a flumazenil insensitive manner regardless of the incorporation of γ subunits, indicating that CGS 9895 targets on non-BZ-binding sites of GABAARs38. Interestingly, CGS 9896, which is a structural analog of CGS 9895, exhibited a similar manner to 6-Methylflavone in a pharmacophore model of benzodiazepine site binding34. Given that the inhibition by luteolin, apigenin and quercetin is independent from γ incorporation and is insensitive to flumazenil, it is reasonable to speculate the possibility that these flavones might interact with the newly identified CGS 9895-binding site at the α(+)/β(−) interface. Our results showed that luteolin had more potent effects on the αβ compared with the αβγ receptors, agreeing with the fact that the αβ receptors embrace two α(+)/β(−) interfaces while the αβγ form only contains one α(+)/β(−) interface. However, the limited information about the exact molecular location of CGS 9895-binding site precludes further investigation of this hypothesis. In addition, we cannot rule out the possibility that luteolin targets on other modulation sites like the neurosteroid-binding site, which is located at the transmembrane domains of GABAARs.
Methods
cDNA constructs and transfection
Rat GABAAR α1, α5, β2 and γ2 subunits were subcloned into the pCDNA3.1 expression vector. HEK293T cells (6 × 105) were transfected with purified plasmids encoding GABAARs (total plasmid amount 2–2.5 μg) by electroporation (NEPA21, NEPA GENE). A small amount (0.2 μg) of pcDNA3-GFP was co-transfected along with GABAARs to act as a transfection marker and facilitate the visualization of transfected cells during electrophysiological experiments. After transfection, cells were re-plated on poly-(D-lysine)-coated glass coverslips and were grown in DMEM in 24-well plates for 16–24 h before patch-clamp recordings.
Slice preparation
Hippocampal slices were prepared from 12–21-day-old ICR mice. Treatments of animals were evaluated and approved by the Institutional Animal Care and Use Committee of China Medical University according to Care of the animals and surgical procedures of China Medical University Protocols. Mice were anaesthetized with Urethane and decapitated. Brains were removed into ice-cold slicing solution containing (in mM): 230 sucrose, 26 NaHCO3, 10 D-glucose, 2.5 KCl, 1.25 NaH2PO4, 0.5 CaCl2 and 10 MgSO4. 350 μm-thick transverse hemi-sections from hippocampus were sliced (Leica vibratome) in the slicing solution. Then the slices were transferred to a storage chamber or the recording chamber with fresh artificial cerebrospinal fluid (ACSF) containing the following (in mM): 128 NaCl, 2.5 KCl, 2.0 MgCl2, 2.0 CaCl2, 1.25 NaH2PO4, 26 NaHCO3 and 10 D-glucose and were incubated at room temperature for >1 h before recording. All solutions were saturated with 95% O2/5% CO2.
Electrophysiology
For recording HEK cells, whole-cell patch clamp recordings were performed under voltage-clamp mode using the AXOPATCH 200B amplifier (Molecular Devices, USA). Whole-cell currents were recorded with a holding potential of −60 mV and signals were acquired via a Digidata 1440A analog-to-digital interface and were low-pass filtered at 2 kHz and digitized at 10 kHz. Patch electrodes (3–7 MΩ) were pulled from 1.5 mm outer diameter thin-walled glass capillaries in three stages on a Flaming-Brown micropipette puller and were filled with intracellular solutions (ICS), which contained (in mM) 140 CsCl, 10 HEPES, 4 Mg-ATP and 0.5 BAPTA (pH 7.20, osmolarity, 290–295 mOsm). The coverslips were continuously superfused with the extracellular solution containing (in mM): 140 NaCl, 5.4 KCl, 10 HEPES, 1.0 MgCl2, 1.3 CaCl2 and 20 glucose (pH 7.4, 305-315 mOsm). To evoke GABA currents, we used fast perfusion of GABA with a computer-controlled multibarrel fast perfusion system (Warner Instruments, CT, USA). For recording hippocampal neurons, slices were perfused with ACSF. Hippocampal CA1 pyramidal neurons were recorded with whole-cell patch clamp with a holding potential of −60 mV under voltage-clamp model using the MultiClamp 700B amplifier (Molecular Devices, USA). Recording pipettes (3–7 MΩ) were filled with the ICS that was mentioned above. For testing the effects of luteolin, slices were pretreated with luteolin for 5–10 min. For recording miniature inhibitory postsynaptic currents (IPSCs), all bath solutions (ACSF) contained 0.5 μM TTX and 20 μM CNQX. The seal tests were performed through the application of a −5 mV step about every 5 min to monitor the changes in access resistance. Data were collected only if the whole-cell access resistance was consistent throughout the recording (changes < 15%). All experiments were performed at 23–25 °C.
Data analysis
Values are expressed as mean ± s.e.m. One-way ANOVA or a two-tailed Student’s t-test was used for statistical analysis and P values less than 0.05 were considered to be statistically significant. Peak current amplitude and 10–90% rises time of recombinant GABAAR-mediated response was measured by Clmapfit 10. Maximum currents (Imax) were determined as the amplitude of peak currents induced by saturated concentration or indicated concentration of agonists. Dose-response curves were created by fitting data to the Hill equation: I = Imax/[1 + (EC50/[A])nH], where I is the current, [A] is a given concentration of agonist and nH is the Hill coefficient (GraphPad Prism 6, CA, USA). The amplitude, frequency and rise time of mIPSCs were measured by MiniAnalysis. The amplitude of tonic currents was revealed by measuring the change in the holding current evoked by applying the GABAAR antagonist bicuculline. The baseline current of 10 s was selected for each treatment and was analyzed by generating an all-points histogram and fitting a Gaussian distribution to the positive side of the histogram by Clampfit 10. The means of the fitted Gaussian were used to determine the holding current before and after drug application39.
Additional Information
How to cite this article: Shen, M.-L. et al. Luteolin inhibits GABAA receptors in HEK cells and brain slices. Sci. Rep. 6, 27695; doi: 10.1038/srep27695 (2016).
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Acknowledgements
This study was supported by grants to NZ and DCW from the Taiwan National Science Council (NSC 102-2320-B-039-038-MY3), the Ministry of Science and Technology (MOST 104-2320-B-039-045-MY3 and MOST 104-2320-B-039-048-MY3), China Medical University (CMU104-S-14-05) and the Ministry of Health and Welfare: Clinical Trial and Research Center of Excellence (MOHW105-TDU-B-212-133019). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
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M.-L.S. and C.-H.W. performed the HEK cell experiments and analyzed the data. R.Y.-T.C. performed brain slice experiments, analyzed the data and edited the manuscript. M.-L.S., N.Z., S.-T.K. and D.C.W. conceived the study. N.Z., S.-T.K. and D.C.W. supervised the experiments and wrote the manuscript.
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Shen, ML., Wang, CH., Chen, RT. et al. Luteolin inhibits GABAA receptors in HEK cells and brain slices. Sci Rep 6, 27695 (2016). https://doi.org/10.1038/srep27695
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DOI: https://doi.org/10.1038/srep27695
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