Abstract
Hepatitis B was still a worldwide health problem. This study aimed to conducted a systematic review and meta-analysis to assess a more precise estimation of factors that influence the response to hepatitis B vaccine in adults. Our included studies examined seroprotection rates close to the end of vaccination schedules in healthy adult populations. This meta-analysis including 21053 adults in 37 articles showed that a significantly decreased response to hepatitis B vaccine appeared in adults (age ≥ 40) (RR:1.86, 95% CI:1.55–2.23), male adults (RR:1.40, 95% CI:1.22–1.61), BMI ≥ 25 adults (RR:1.56, 95% CI:1.12–2.17), smoker (RR:1.53, 95% CI:1.21–1.93), and adults with concomitant disease (RR:1.39, 95% CI:1.04–1.86). Meanwhile, we further found a decreased response to hepatitis B vaccine appeared in adults (age ≥ 30) (RR:1.77, 95% CI:1.48–2.10), and adults (age ≥ 60) (RR:1.30, 95% CI:1.01–1.68). However, there were no difference in response to hepatitis B vaccine both in alcoholic (RR:0.90, 95% CI:0.64–1.26) and 0-1-12 vs. 0-1-6 vaccination schedule (RR:1.39, 95% CI:0.41–4.67). Pooling of these studies recommended the sooner the better for adult hepatitis B vaccine strategy. More vaccine doses, supplemental/additional strengthening immunity should be emphasized on the susceptible population of increasing aged, male, BMI ≥ 25, smoking and concomitant disease. The conventional 0-1-6 vaccination schedule could be still worth to be recommended.
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Introduction
Hepatitis B as an acute and chronic communicable disease, has been a worldwide health problem estimated to lead to between 500,000 to 1.2 million deaths every year through causing chronic hepatitis, cirrhosis and hepatocellular carcinoma1. The prevalence of HBV infection varies significantly in different areas: prevalence of chronic infection with HBV estimates range between 0.1–0.7% in Western, Northern, and Central Europe, while those considerably higher in Eastern and Southern European countries, such as Italy (0.2–4.3%), Turkey (2.5–9%), and Romania (5.6%)2,3. In Alaska, 41% had anti-HBs levels of >10 mIU/ml 7 to 9 years after booster vaccination at birth4, even 51% had this protective levels 30 years after receiving the primary series without subsequent doses in Alaska native persons5. In China, the HBsAg carrier rate was 8.75% in 1979, 9.75% in 1992, and 7.18% in 20066; in Taiwan, the values are as high as 15–20% in adults7; and in the Middle East and North Africa region, the HBV infection estimates are various such as 9.8% in Egypt, 7.4% in Iran, 2.4% in Lebanon and 6.9% Libya from the prisoners; 50.7% in Iran, 8.6% in Israel, 2.8% in Lebanon, 4.5% in Libya, 2.6% in Palestine, 6.1% in Saudi Arabia from the injecting drug users8. In Gambia, 13.2% were found to carry HbsAg9 and national infant HBV vaccination controlling chronic infection had 94% vaccine efficacy10. HBV can be transmitted in many ways, with sexual intercourse and mother-to-child transmission being the most common. Between 15% and 40% of those infected develop acute or chronic liver disease and liver failure, cirrhosis or hepatocellular carcinoma may result.
Many countries have gradually adopted the HBV vaccine in national immunization programs since the World Health Organization (WHO) recommended vaccination for children in 1990s. Most individuals with chronic hepatitis B are asymptomatic and therefore ignorant of their infection status but HBV vaccination, if used for primary prevention, can significantly lower the risk of infection. HBV vaccination triggers antibody response and antibody to hepatitis B surface antigen (anti-HBs) levels ≥10 IU/L are usually regarded as seroprotection for most vaccinees.
Vaccination efficacy among children has been widely studied, but there remains a large proportion of adult populations who are as yet unvaccinated. A previous meta-analysis in 2002 observed many factors influencing response to hepatitis B vaccine, especially a decrease response to recombinant HBV vaccine at higher ages11, which suggested that earlier vaccination should be prioritized for prevention at the population level. However, in the last decade, numerous emerging reports, which focused on the seroprotection rate of hepatitis B vaccine in adults12,13,14,15,16,17,18,19,20,21,22,23,24,25,26,27,28,29,30,31,32,33,34,35,36,37,38,39,40,41,42,43,44,45,46,47,48, are still inconclusive to immunize what adults are the most appropriate in order to increase the seroprotection rate. Factors influencing immunologic response to hepatitis B vaccine in adults have been inconsistently examined in existing studies. In this study, we conducted a systematic review and meta-analysis to update and assess a more precise estimation of factors that influence the response to HBV vaccine.
Material and Methods
Search strategy
To find all relevant publications that investigated the association between adult and hepatitis B vaccine and seroprotection, a systematic literature search was independently conducted by two individual investigators with the same method in PubMed, Embase and Cochrane Library using the keywords “hepatitis B vaccine”, “HBV”, “adult”, “anti-HBs” were used. Data were collected from the full-published paper and no language or race restriction was used. Bibliographies of relevant review articles were also screened to supplement the electronic searches.
Inclusion criteria
Included studies met the following criteria (1) original research papers; (2) prospective or retrospective studies, including cohorts and trials; (3) sample size ≥10; (4) healthy subjects, pregnant women, participants with diabetes, chronic renal failure or other diseases but without congestive hepatopathy or infectious diseases; (5) mean sample population age ≥18 years; (6) populations are largely vaccine naive; (7) seroprotection (generally defined as antibody-HBs at a titer of >10 mlU/mL) is assessed at least one month after last recombinant vaccine dose in the majority of participants.
Article screening
Citations were electronically downloaded into reference management software and duplicate citations were electronically/manually excluded. Where studies had multiple reports, the most recent or most complete article was retained. The remaining citations were screened independently by two reviewers using pre-defined criteria. Full-text versions of potentially relevant citations were obtained and again screened independently by two reviewers according to pre-defined criteria. Disagreement was resolved by the opinion of a third reviewer.
Data extraction and quality assessment
The data was independently extracted and then cross-checked by two investigators according to a standard format as follows: author, publication year, age, country, male/female participants, body mass index (BMI), vaccination schedule, time of immunological assessment after last vaccine dose, vaccine characteristics and injection pathwayIf necessary data were unavailable in articles, a request was sent to the author for relevant data. The definition of age strata varied among studies. We considered age ≥16 years as adult and age ≥40 years as a default definition of older age for study participants. We considered individuals with anti-HBs titers ≥10 IU/L to be seroprotective after completion of vaccination against HBV. In a few articles, those data are also available if the seroconversion was defined as anti-HBs titers ≥10 IU/L. The articles were divided into four quality levels such as high, moderate, low, and very low by GRADE evidence profile, which allocates original ranks of low score to observational studies and high score to RCTs49.
Statistical analysis
In this meta-analysis, we calculated the relative risks (RRs) and 95% confidence intervals (CIs) by comparing the valid and invalid participators in the experimental group and control group of recruited articles. Statistical heterogeneity in the studies was examined by the Q statistic. We evaluated the heterogeneity in these studies by this method, I2 = 100 %*(Q-df)/Q. A fixed-effect model was used to analyze the data if there was no statistical difference of heterogeneity (p ≥ 0.05). Otherwise, a random-effect model would be selected.
Subgroups analyses were defined in advance/defined according to the reported data, and studies or results were grouped according to age (older or younger than 40), sex, smoking status, alcoholism, vaccine administration (0-1-12/0-1-6 vaccination schedule), geographical location (Asians/Non-Asian). Sensitivity analysis was performed to estimate the stability of the model by removing each study in turn. Additionally, publication bias was assessed through the funnel plot and Egger’s linear regression test50. All statistical analyses were conducted by Stata 12.0 software.
Results
Characteristics of eligible studies
We finally identified 21053 adults from 37 articles up to June 30, 2015 through electronic and manual searches (Fig. 1). Nine hundred and forty-six studies were excluded according to the mentioned criteria. The characteristics of included studies for this meta-analysis are presented in Table 1 and the majority of the studies were assessed as being of good quality (Table 2).
The studies were largely prospective cohort (n = 5), retrospective cohorts (n = 23), or randomized trials (n = 10). Studies varied considerably in size and were conducted among many countries. The three vaccine doses tended to be administered either at months 0, 1, 6 or 0, 1, 12 and recombinant vaccine doses ranged from 10 μg to 40 μg.
Meta-analysis results
Heterogeneity test result and subgroup analysis
The Q-tests of heterogeneity were marked in partial groups and then the pooled RRs were calculated by the random-effect models and fixed-effect models. Meta-analysis revealed that vaccine non-response rates were significantly greater in older participants (age ≥40 vs. <40 years, RR:1.86, 95% CI:1.55 to 2.23, I2 = 56%, P = 0.001; age ≥30 vs. <30 years, RR:1.77, 95% CI:1.48 to 2.10, I2 = 37%, P = 0.074; age ≥60 vs. age<60 years RR:1.30, 95% CI:1.01 to 1.68 I2 = 33.4%, P = 0.199). Non-response was also more likely among males (male adults vs. female adults, RR:1.40, 95% CI:1.22 to 1.61, I2 = 44.3%, P = 0.005); overweight participants (BMI ≥ 25 adults vs. <25, RR:1.56, 95% CI:1.12 to 2.17, I2 = 77.3%, P < 0.001); smokers (smoker vs. nonsmoker, RR:1. 53, 95% CI:1.21 to 1.93, I2 = 52.1%, P = 0.01) and those with concomitant disease compared to healthy participants (RR:1.39, 95% CI:1.04 to 1.86, I2 = 63.4%, P = 0.002) (Figs 2a, 3a, 4a,b,d and 5a,d). However, there were no differences in response to HBV by alcoholic status (alcoholic vs. nonalcoholic, RR:0.90, 95% CI:0.64 to 1.26, I2 = 0, P = 0.941) or vaccination schedule (vaccine delivered at months 0-1-12 vs. 0-1-6, RR:1.39, 95% CI:0.41 to 4.67, I2 = 77.4%, P = 0.004).
(a) The relative risks of response to HBV vaccine between adults age ≥40 and adults age <40. (b) The relative risks of response to HBV vaccine between adults age ≥40 and adults age <40. Comparing with adults age <40, the RRs show decreased response to HBV vaccine among adults age ≥40 in cohort and overall studies.
(a) The relative risks of response to HBV vaccine between adults age ≥30 and adults age <30. (b) The relative risks of response to HBV vaccine between adults age ≥30 and adults age <30 grouped by study design. Comparing with adults age <30, the RRs indicate reduced response to HBV vaccine among adults age ≥30 both in cohort and RCT studies.
(a) The relative risks of response to HBV vaccine between adults age ≥60 and adults age <60. (b) The relative risks of response to HBV vaccine between male adults and female adults. (c) The relative risks of response to HBV vaccine between male adults and female adults grouped by study design. Comparing with female adults, The RRs suggest declined response to HBV vaccine among male adults both in cohort and RCT studies. (d) The relative risks of response to HBV vaccine between BMI ≥25 adults and BMI <25 adults.
(a) The relative risks of response to HBV vaccine between smoker and nonsmoker. (b) The relative risks of response to HBV vaccine between alcoholic and nonalcoholic. (c) The relative risk of response to HBV vaccine between adults with concomitant disease and healthy adults. (d) The relative risk of response to HBV vaccine between 0-1-12 and 0-1-6 vaccination schedule.
Subgroup analysis by study location and age indicates that older adults (≥40 years) from non-Asian countries revealed that in contrast with Asians, specially non-Asians in older adults (age ≥ 40) may be slightly less response to hepatitis B vaccine than younger adults (age < 40) (RR:2.02, 95% CI:1.59–2.58; RR:1.60, 95% CI:1.24–2.08), consistent with the region result of older adults (age ≥ 30) (RR:2.16, 95% CI:1.66–2.80; RR:1.46, 95% CI:1.15–1.86). Particularly comparing with Asians, the male in non-Asians has a similar nonresponse to females (RR:1.42, 95% CI:1.18–1.71; RR:1.40, 95% CI:1.11–1.77).
When studies were subdivided by study design results were consistent and lower response was seen among studies with older participants and male participants and again, no difference was observed by alcoholic status (Figs 2b, 3b, 4c,d and 5a,b).
Sensitivity analysis and publication bias
Sensitivity analysis was conducted to estimate the stability of the results and indicated no significant change if any one study was excluded. Funnel plot asymmetry was assessed by means of Egger’s linear regression test and showed that there was significant publication bias in the following groups: age of 40 years, gender and BMI (age40: t = 2.54, P = 0.019; sex: t = 2.99, P = 0.006; BMI: t = 2.70, P = 0.025).
Discussion
When stratified by demographic features, our study showed a lower response in older adults (especially age ≥ 40), male adults and overweight adults (BMI ≥ 25), smoker and adults with concomitant disease after completion of vaccination against hepatitis B.
Our study indicated that young adults have a higher seroprotection rate to hepatitis B vaccine than other age groups (age30: RR = 1.77; age40: RR = 1.86; age60: RR = 1.30). It means that the earlier adult vaccination was inoculated at an age, the better efficiency is. The lower responsiveness to hepatitis B vaccines in older adults might result from the waning immunity with age. In previous studies, it did not find a significant association between age and the immune response43,44. The reason may be that most adults in the study were under the age of 40 years. However, in an observational prospective study of 666 participants, the percentage of nonresponders elevated gradually with age51. Another study aligned with our findings also found that younger age and female gender were predictive of better response52. It indicated in our study that population in non-Asians were both better in age of 40 or 30 years (age30: RR = 2.16; age40: RR = 2.06). Surprisingly our result also showed that the response rate in the younger adults (age < 60) was better than those older (age ≥ 60), different from the previous study44. Some studies reported seroprotection rates of hepatitis B vaccine in older adults (aged ≥ 60 years) range from 30% to 80% and rely on these factors such as study population, vaccination plan, vaccination history and type of vaccine40.
Besides age in our study, male gender both in Asians and non-Asians may be associated with nonresponse to hepatitis B vaccine. It may be owing to the opposite effects of sex hormone androgen and estrogen. This difference is experimentally repeated in animal models, which indicated to be activated by sex hormones in genetic regulation. Moreover, there are numerous immunological genes appearing on the X chromosome while few ones are mapped on the Y chromosome. Estrogen activates monocytes to secrete IL-10, which induces IgG and IgM secretion through B-cells in turn53, while testosterone damages the production of IgG and IgM from B-lymphocytes, as well as restrains producing IL-6 from monocytes54. The hormones’ joint effects on the epigenetic adjustment of genetic expression, and gene structure on the X chromosome differing between XX females and XY males, will partly account for vaccine response heterogeneity in gender55. Based on our results, future programme should be emphasized on males both Asians and non-Asians, who tend to have less response to hepatitis B vaccine.
BMI might influence the level of vaccine response25. The low response to vaccination of overweight on vaccine could be due to the main distribution of the vaccine in fat not in muscle. This could hinder absorption and enable denaturation of the vaccine antigen by enzymatic action25. Another possible interpretation is damaged proliferation and function of the antibody-secreting plasma cells.
The lower immunogenicity of hepatitis B vaccine was linked with smoking and male gender. In smokers, smoking can affect cells and humoral mediated immune responses in humans and animals. Nicotine restrains the antibody-forming cell response by damaging antigen-mediated pathway in T cells and intracellular calcium response. In addition, a high prevalence of HBV markers has been reported in alcoholics. Persistent alcohol intake could restrain immune responses especially in female56. But some studies also reported that difference was undetected between alcohol consumption and seroprotection of hepatitis B vaccination31,38. In this study, the inapparent association within alcoholic subgroup may result from the small sample size and drinking is not common in females.
Aside from those factors, patients with concomitant disease usually have a complicated and inconstant status due to diverse pathogenesis. Although some researches found no association between comorbidity and seroprotection36,37, comorbidity may be a significant element decreasing the efficacy of hepatitis B vaccine from this analysis and others23,27, which could bring immunity disturbance. However, the detailed mechanisms between the poor response to hepatitis B vaccine and adults suffering from concomitant disease are still incompletely understood.
What’s more, the four reports regarding different vaccination schedules in adults such as 0-1-6 and 0-1-12 schedules are still controversial13,39,47,48. Three studies among them13,39,47 observed no difference in seroconversion rates between these two schedules (as in these cases they were all nearly 100%), while another study48 showed a higher seroconversion rate in individuals with the 0-1-12 schedule. Our meta analysis found no difference for seroconversion rate one month after the third injection both in 0-1-6 and 0-1-12 vaccination schedules. Thus in consideration of timing and vaccination compliance, the conventional 0-1-6 vaccination schedule could be still worth to be recommended.
Recently, emerging studies tended to suggest the genetic determinants of heterogeneity in response to the vaccines against hepatitis B. In twins study, 60% of the phenotypic variance was interpreted for the anti-HBs immune response by additive genetic while 40% by non-shared environmental effects57. Asians and non-Asians as study location may also play an important role in seroprotection efficiency of hepatitis B vaccine in adults. The percentage of nonresponders after hepatitis B vaccine remarkably varied in ethnic groups, which may result from the difference of environmental surroundings, the mutation rate and genetic variability, especially at the human leucocyte antigen (HLA) genetic region. However, it is also hard to accurately locate the variation affecting the HBV response in the HLA locus as a result of the long-range linkage disequilibrium in this area58. It needs further studies to explore.
In a word, the factors mentioned above suggested these factors consisting of elder adults, male, BMI ≥ 25, smoking and concomitant disease would be the significant variables reducing the immune response to hepatitis B vaccination. Those who are more likely to have non-response should be checked for seroprotection level and offered additional booster vaccinations. Thereby finding those without immunization and improving overall immunization rates across the population should be emphasized.
Our results should be interpreted in view of the following limitations. First, publication bias was identified among studies reporting rates by age groups, gender and BMI groups. Second, for the various subgroup analyses, sample size is diminished and therefore CIs are wide leading to less accurate estimates of response. Third, due to differences in lifestyle characteristics in different studies’ population, significant heterogeneity was present in study, even among subgroup estimates. In addition, there were poor reporting in some included studies and limited inclusion in subgroup analyses such as BMI, smoking status, alcohol status and concomitant disease. What’s more, several different vaccines were used in the different studies, which had different immunogenicity. Engerix B (with 20 μg HBsAg per dose) is more immunogenic than Recombivax (with 10 μg per dose), the difference being seen especially in older individuals. Twinrix is similar immunogenicity to Engerix-B. A multi-center study found that a S-PreS1/PreS2-vaccine (Hepacare) is also more immunogenic than Engerix B2. Despite limitations, in this work, we systematically sought out all published literature relevant to our research question and then carefully screened studies and extracted data in duplicate using protocols to ensure high quality and consistency in the extracted data. Missing data were sought from authors and studies results were statistically combined to provide robust estimates of the factors associated with poorer immunological response. To our knowledge, this is the first study to examine multiple factors associated with vaccine response and important differences have been found.
Conclusions
Taken together, this meta-analysis indicated that there were lower seroprotection rates to hepatitis B vaccine in the subgroups of increasing aged adults, male, BMI ≥ 25, smoking and concomitant disease, and more vaccine doses, supplemental/additional strengthening immunity should be focused on this specific population. No difference in seroconversion rates between 0-1-6 and 0-1-12 vaccination schedule was observed, but in consideration of timing and vaccination compliance, the vaccination 0-1-6 schedule could be still worth to be recommended. In order to obtain accurate effectiveness of hepatitis B vaccine in adults, more large-scale studies should be conducted in the future.
Additional Information
How to cite this article: Yang, S. et al. Factors influencing immunologic response to hepatitis B vaccine in adults. Sci. Rep. 6, 27251; doi: 10.1038/srep27251 (2016).
References
Lavanchy, D. Hepatitis B virus epidemiology, disease burden, treatment, and current and emerging prevention and control measures. Journal of viral hepatitis 11, 97–107 (2004).
European Centre for Disease Prevention and Control. Hepatitis B and C in the EU neighbourhood: prevalence, burden of disease and screening policies. European Centre for Disease Prevention and Control. 56 p. doi: 10.2900/30933 (Stockholm, 2010).
Hahne, S. J. et al. Infection with hepatitis B and C virus in Europe: a systematic review of prevalence and cost-effectiveness of screening. BMC infectious diseases 13, 181, doi: 10.1186/1471-2334-13-181 (2013).
Keck, J. W. et al. Hepatitis B virus antibody levels 7 to 9 years after booster vaccination in Alaska native persons. Clinical and vaccine immunology: CVI 21, 1339–1342, doi: 10.1128/CVI.00263-14 (2014).
Bruce, M. G. et al. Antibody Levels and Protection After Hepatitis B Vaccine: Results of a 30-Year Follow-up Study and Response to a Booster Dose. The Journal of infectious diseases, doi: 10.1093/infdis/jiv748 (2016).
Yang, S. G. et al. Effectiveness of HBV vaccination in infants and prediction of HBV prevalence trend under new vaccination plan: findings of a large-scale investigation. PloS one 7, e47808, doi: 10.1371/journal.pone.0047808 (2012).
Su, F. H. et al. Hepatitis B seroprevalence and anamnestic response amongst Taiwanese young adults with full vaccination in infancy, 20 years subsequent to national hepatitis B vaccination. Vaccine 25, 8085–8090, doi: 10.1016/j.vaccine.2007.09.013 (2007).
Melhem, N. M., Rahhal, N., Charide, R., Kreidieh, K. & El-Khatib, R. Human immunodeficiency virus and viral hepatitis among high-risk groups: Understanding the knowledge gap in the Middle East and North Africa Region. World journal of hepatology 7, 2619–2630, doi: 10.4254/wjh.v7.i25.2619 (2015).
McGregor, I. A. Health and Communicable Disease in a Rural African Environment. Oikos 27, 180–192, doi: 10.2307/3543897 (1976).
Peto, T. J. et al. Efficacy and effectiveness of infant vaccination against chronic hepatitis B in the Gambia Hepatitis Intervention Study (1986–90) and in the nationwide immunisation program. BMC infectious diseases 14, 7, doi: 10.1186/1471-2334-14-7 (2014).
Fisman, D. N., Agrawal, D. & Leder, K. The effect of age on immunologic response to recombinant hepatitis B vaccine: a meta-analysis. Clinical infectious diseases: an official publication of the Infectious Diseases Society of America 35, 1368–1375, doi: 10.1086/344271 (2002).
Zajac, B. A., West, D. J., McAleer, W. J. & Scolnick, E. M. Overview of clinical studies with hepatitis B vaccine made by recombinant DNA. The Journal of infection 13 Suppl A, 39–45 (1986).
Jilg, W., Schmidt, M. & Deinhardt, F. Vaccination against hepatitis B: comparison of three different vaccination schedules. The Journal of infectious diseases 160, 766–769 (1989).
Morris, C. A., Oliver, P. R., Reynolds, F. & Selkon, J. B. Intradermal hepatitis B immunization with yeast-derived vaccine: serological response by sex and age. Epidemiology and infection 103, 387–394 (1989).
Westmoreland, D., Player, V., Heap, D. C. & Hammond, A. Immunization against hepatitis B–what can we expect? Results of a survey of antibody response to immunization in persons 'at risk' of occupational exposure to hepatitis B. Epidemiology and infection 104, 499–509 (1990).
Guan, R., Tay, H. H., Choong, H. L., Yap, I. & Woo, K. T. Hepatitis B vaccination in chronic renal failure patients undergoing haemodialysis: the immunogenicity of an increased dose of a recombinant DNA hepatitis B vaccine. Annals of the Academy of Medicine, Singapore 19, 793–797 (1990).
Dahl-Hansen, E., Siebke, J. C., Froland, S. S. & Degre, M. Immunogenicity of yeast-derived hepatitis B vaccine from two different producers. Epidemiology and infection 104, 143–149 (1990).
Dentico, P. et al. Long-term immunogenicity safety and efficacy of a recombinant hepatitis B vaccine in healthy adults. European journal of epidemiology 8, 650–655 (1992).
Roome, A. J., Walsh, S. J., Cartter, M. L. & Hadler, J. L. Hepatitis B vaccine responsiveness in Connecticut public safety personnel. Jama 270, 2931–2934 (1993).
McMaster, K. R., 3rd, Roper, J. K. & Carter, J. B. Intradermal hepatitis B vaccination in a 300-bed primary care hospital: experience with a recombinant vaccine in a four-dose schedule. American journal of infection control 21, 283–288 (1993).
Jaiswal, S. B., Salgia, P. B., Sepaha, A. G. & Chitnis, D. S. Hepatitis B vaccine in patients on haemodialysis. Lancet 346, 317–318 (1995).
Bock, H. L. et al. Immunogenicity of a recombinant hepatitis B vaccine in adults. Archives of internal medicine 156, 2226–2231 (1996).
Averhoff, F. et al. Immunogenicity of hepatitis B Vaccines. Implications for persons at occupational risk of hepatitis B virus infection. American journal of preventive medicine 15, 1–8 (1998).
Cardell, K., Fryden, A. & Normann, B. Intradermal hepatitis B vaccination in health care workers. Response rate and experiences from vaccination in clinical practise. Scandinavian journal of infectious diseases 31, 197–200 (1999).
Ingardia, C. J., Kelley, L., Steinfeld, J. D. & Wax, J. R. Hepatitis B vaccination in pregnancy: factors influencing efficacy. Obstetrics and gynecology 93, 983–986 (1999).
Young, M. D. et al. Adult hepatitis B vaccination using a novel triple antigen recombinant vaccine. Hepatology 34, 372–376, doi: 10.1053/jhep.2001.26167 (2001).
Wolters, B., Junge, U., Dziuba, S. & Roggendorf, M. Immunogenicity of combined hepatitis A and B vaccine in elderly persons. Vaccine 21, 3623–3628 (2003).
Martins, R. M. et al. Multicenter study on the immunogenicity and safety of two recombinant vaccines against hepatitis B. Memorias do Instituto Oswaldo Cruz 99, 865–871, doi: S0074-02762004000800014 (2004).
Yen, Y. H. et al. Study of hepatitis B (HB) vaccine non-responsiveness among health care workers from an endemic area (Taiwan). Liver international: official journal of the International Association for the Study of the Liver 25, 1162–1168, doi: 10.1111/j.1478-3231.2005.01157.x (2005).
Panhotra, B. R., Saxena, A. K., Al-Hamrani, H. A. & Al-Mulhim, A. Compliance to hepatitis B vaccination and subsequent development of seroprotection among health care workers of a tertiary care center of Saudi Arabia. American journal of infection control 33, 144–150, doi: 10.1016/j.ajic.2005.01.002 (2005).
Kulkarni, P. S. et al. Immunogenicity of a new, low-cost recombinant hepatitis B vaccine derived from Hansenula polymorpha in adults. Vaccine 24, 3457–3460, doi: 10.1016/j.vaccine.2006.02.008 (2006).
Estevez, Z. C. et al. Immunogenicity and safety assessment of the Cuban recombinant hepatitis B vaccine in healthy adults. Biologicals: journal of the International Association of Biological Standardization 35, 115–122, doi: 10.1016/j.biologicals.2006.06.001 (2007).
Locquet, C., Marande, J. L., Choudat, D. & Vidal-Trecan, G. Hepatitis B vaccination in women healthcare workers: a seroepidemiological survey. European journal of epidemiology 22, 113–119, doi: 10.1007/s10654-006-9094-x (2007).
Sabido, M., Gavalda, L., Olona, N. & Ramon, J. M. Timing of hepatitis B vaccination: its effect on vaccine response in health care workers. Vaccine 25, 7568–7572, doi: 10.1016/j.vaccine.2007.08.025 (2007).
Oliveira, L. C., Silva, T. E. & Alves, M. H. Response to hepatitis B vaccine in alcoholics without clinically evident liver cirrhosis. Arquivos de gastroenterologia 44, 195–200 (2007).
Wolters, B. et al. Comparative evaluation of the immunogenicity of combined hepatitis A and B vaccine by a prospective and retrospective trial. Human vaccines 5, 248–253 (2009).
Kevorkyan, A. K. et al. Immune response and immunologic memory in medical personnel vaccinated with hepatitis B vaccine. Folia medica 53, 32–38 (2011).
Sheffield, J. S. et al. Efficacy of an accelerated hepatitis B vaccination program during pregnancy. Obstetrics and gynecology 117, 1130–1135, doi: 10.1097/AOG.0b013e3182148efe (2011).
De Schryver, A. et al. Comparative immunogenicity of two vaccination schedules of a combined hepatitis A and B vaccine in healthy volunteers. Journal of viral hepatitis 18, e5–10, doi: 10.1111/j.1365-2893.2010.01365.x (2011).
Tohme, R. A. et al. Evaluation of hepatitis B vaccine immunogenicity among older adults during an outbreak response in assisted living facilities. Vaccine 29, 9316–9320, doi: 10.1016/j.vaccine.2011.10.011 (2011).
Ren, J. J. et al. Immunological effects of a 10-mug dose of domestic hepatitis B vaccine in adults. Journal of Zhejiang University . Science. B 13, 948–954, doi: 10.1631/jzus.B1200179 (2012).
Williams, R. E. et al. Hepatitis B vaccination of susceptible elderly residents of long term care facilities during a hepatitis B outbreak. Vaccine 30, 3147–3150, doi: 10.1016/j.vaccine.2012.02.078 (2012).
Chathuranga, L. S., Noordeen, F. & Abeykoon, A. M. Immune response to hepatitis B vaccine in a group of health care workers in Sri Lanka. International journal of infectious diseases: IJID: official publication of the International Society for Infectious Diseases 17, e1078–1079, doi: 10.1016/j.ijid.2013.04.009 (2013).
Bender, T. J. et al. Hepatitis B vaccine immunogenicity among adults vaccinated during an outbreak response in an assisted living facility–Virginia, 2010. Vaccine 32, 852–856, doi: 10.1016/j.vaccine.2013.12.018 (2014).
Thomas, R. J. et al. Prevalence of non-responsiveness to an indigenous recombinant hepatitis B vaccine: a study among South Indian health care workers in a tertiary hospital. Indian journal of medical microbiology 33 Suppl, 32–36, doi: 10.4103/0255-0857.150877 (2015).
Nashibi, R. et al. Post-vaccination Immunity Against Hepatitis B Virus and Predictors for Non-responders Among Medical Staff. Jundishapur journal of microbiology 8, e19579, doi: 10.5812/jjm.19579 (2015).
Yao, J. et al. Optimal vaccination program for healthy adults in China. Human vaccines & immunotherapeutics, 0, doi: 10.1080/21645515.2015.1053674 (2015).
Yao, J. et al. The response of hepatitis B vaccination on seronegative adults with different vaccination schedules. Human vaccines & immunotherapeutics 11, 1102–1107, doi: 10.4161/21645515.2014.985500 (2015).
Guyatt, G. et al. GRADE guidelines: 1. Introduction-GRADE evidence profiles and summary of findings tables. Journal of clinical epidemiology 64, 383–394, doi: 10.1016/j.jclinepi.2010.04.026 (2011).
Egger, M., Davey, S. G., Schneider, M. & Minder, C. Bias in meta-analysis detected by a simple, graphical test. BMJ. Bmj 315, 477–477 (1997).
Zeeshan, M. et al. Evaluation of immune response to Hepatitis B vaccine in health care workers at a tertiary care hospital in Pakistan: an observational prospective study. BMC infectious diseases 7, 120, doi: 10.1186/1471-2334-7-120 (2007).
Sangfelt, P., Uhnoo, I., Reichard, O. & Weiland, O. A low-dose intradermal hepatitis B vaccine programme in health-care workers and students is highly effective and cost saving: a retrospective follow-up survey in the clinical setting. Scandinavian journal of gastroenterology 43, 465–472, doi: 10.1080/00365520701733806 (2008).
Kanda, N. & Tamaki, K. Estrogen enhances immunoglobulin production by human PBMCs. The Journal of allergy and clinical immunology 103, 282–288 (1999).
Kanda, N., Tsuchida, T. & Tamaki, K. Testosterone inhibits immunoglobulin production by human peripheral blood mononuclear cells. Clinical and experimental immunology 106, 410–415 (1996).
Klein, S. L., Marriott, I. & Fish, E. N. Sex-based differences in immune function and responses to vaccination. Transactions of the Royal Society of Tropical Medicine and Hygiene 109, 9–15, doi: 10.1093/trstmh/tru167 (2015).
Saunders, J. B., Davis, M. & Williams, R. Do women develop alcoholic liver disease more readily than men? British medical journal 282, 1140–1143 (1981).
Hohler, T. et al. Differential genetic determination of immune responsiveness to hepatitis B surface antigen and to hepatitis A virus: a vaccination study in twins. Lancet 360, 991–995, doi: 10.1016/S0140-6736(02)11083-X (2002).
Mentzer, A. J., O'Connor, D., Pollard, A. J. & Hill, A. V. Searching for the human genetic factors standing in the way of universally effective vaccines. Philosophical transactions of the Royal Society of London. Series B, Biological sciences 370, doi: 10.1098/rstb.2014.0341 (2015).
Acknowledgements
This study was supported by grants from the Mega-Project for National Science and Technology Development under the “11th and 12th Five-Year Plan of China” (2009ZX10004-901, 2011ZX10004-901, 2013ZX10004901, 2013ZX10004904 and 2014ZX10004008).
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Study concept and design; S.Y., G.T., B.R., T.J. and L.L. Acquisition of data; S.Y., G.T., Y.C., C.D., M.D., C.Y., K.X., J.R., J.Y., Y.L., Q.C., P.C., T.X., C.W., B.W., B.R., T.J. and L.L. Analysis and interpretation of data; S.Y., G.T., C.M., Y.C. and C.D. Drafting of the manuscript; S.Y., G.T. and C.M. Critical revision of the manuscript for important intellectual content; S.Y., G.T. and C.M. Statistical analysis; S.Y., G.T., C.M., Y.C. and C.D. Obtained funding; S.Y., G.T., B.R., T.J. and L.L. Technical, or material support; Y.C., C.D., M.D., C.Y., K.X., J.R., J.Y., Y.L., Q.C., P.C., T.X., C.W. and B.W. Study supervision: B.R., T.J. and L.L.
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Yang, S., Tian, G., Cui, Y. et al. Factors influencing immunologic response to hepatitis B vaccine in adults. Sci Rep 6, 27251 (2016). https://doi.org/10.1038/srep27251
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DOI: https://doi.org/10.1038/srep27251
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